Assessing cell migration in hydrogels: An overview of relevant materials and methods

Solbu, Anita Akbarzadeh; Caballero, David; Damigos, Spyridon; Kundu, Subhas C.; Reis, Rui L.; Halaas, Øyvind; Chahal, Aman S; Strand, Berit Løkensgard

doi:10.1016/j.mtbio.2022.100537

Cited by 25 publications

(25 citation statements)

References 179 publications

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“…However, successful 3D cell migration depends not only on the cell type and its inherent ability to adapt to environmental changes but also on the intrinsic properties of the hydrogel, such as stiffness, pore size, and adhesiveness. [ 42 ] Limited migration of cells within a living scaffold hinders the formation of functional tissue structures and leads to graft failure or poor clinical outcomes. [ 10 ] Thus, understanding the factors that regulate cell migration in 3D hydrogels is critical for developing effective strategies to enhance the survival and function of stem cells for tissue engineering applications.…”

Section: Resultsmentioning

confidence: 99%

Fine‐tuning Dynamic Cross–linking for Enhanced 3D Bioprinting of Hyaluronic Acid Hydrogels

Tavakoli,

Krishnan,

Mokhtari

et al. 2023

Adv Funct Materials

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Abstract3D bioprinting of stem cells shows promise for medical applications, but the development of an efficient bioink remains a challenge. Recently, the emergence of dynamically cross–linked hydrogels has advanced this field to obtain self‐healing materials. However, more advanced bioinks are needed that display optimum gelling kinetics, viscoelasticity, shear‐thinning property, structural fidelity, and hold the printed structures sufficiently long enough that allow maturation of the new tissue. Here, a novel extracellular matrix‐based bioink for human mesenchymal stem cells (hMSCs) is presented. Hyaluronic acid (HA) is modified with cysteine and aldehyde functional groups, creating hydrogels with dual cross–linking of disulfide and thiazolidine products. The investigation demonstrates that this cross–linking significantly improves hydrogel stability and biological properties. The bioink exhibits fast gelation kinetics, shear‐thinning, shape‐maintaining properties, high cell survival after printing with >2‐fold increase in stemness marker (OCT3/4 and NANOG), and supports cell proliferation and migration. Disulfide cross–linking contributes to self‐healing and cell migration, while thiazolidine cross–linking reduces gelation time, enhances long‐term stability, and supports cell proliferation. Overall, the HA‐based bioink fulfills the requirements for successful 3D printing of stem cells, providing a promising solution for cell therapy and regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

Fine‐tuning Dynamic Cross–linking for Enhanced 3D Bioprinting of Hyaluronic Acid Hydrogels

Tavakoli,

Krishnan,

Mokhtari

et al. 2023

Adv Funct Materials

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“… Structure of hydrogels where ( A ) ideal network, ( B ) entanglements, and ( C ) hydrogel network micro-structures. Reprinted from Solbu et al [ 75 ]. …”

Section: Figurementioning

confidence: 99%

Injectable Hydrogels for Nervous Tissue Repair—A Brief Review

Politrón-Zepeda,

Fletes-Vargas,

Rodríguez-Rodríguez

2024

Gels

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The repair of nervous tissue is a critical research field in tissue engineering because of the degenerative process in the injured nervous system. In this review, we summarize the progress of injectable hydrogels using in vitro and in vivo studies for the regeneration and repair of nervous tissue. Traditional treatments have not been favorable for patients, as they are invasive and inefficient; therefore, injectable hydrogels are promising for the treatment of damaged tissue. This review will contribute to a better understanding of injectable hydrogels as potential scaffolds and drug delivery system for neural tissue engineering applications.

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“…The interaction between cells and materials is crucial in wound healing, which manifests in promoting cell migration, regulating inflammatory cell differentiation, and enhancing angiogenesis. 34 The effects of hydrogels and mild photothermal stimulation on cell migration were evaluated by a series of scratch tests. To ensure that cell migration is not caused by cell proliferation, L929 cells were respectively starved with low serum-containing culture medium for 24 h before construction of the scratch according to a previous protocol.…”

Section: In Vitro Cell Migration and Angiogenesismentioning

confidence: 99%

Wound microenvironment regulatory poly(l-glutamic acid) composite hydrogels containing metal ion-coordinated nanoparticles for effective hemostasis and wound healing

Chen,

Zhang,

Wang

et al. 2024

Biomater. Sci.

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Assessing cell migration in hydrogels: An overview of relevant materials and methods

Cited by 25 publications

References 179 publications

Fine‐tuning Dynamic Cross–linking for Enhanced 3D Bioprinting of Hyaluronic Acid Hydrogels

Fine‐tuning Dynamic Cross–linking for Enhanced 3D Bioprinting of Hyaluronic Acid Hydrogels

Injectable Hydrogels for Nervous Tissue Repair—A Brief Review

Wound microenvironment regulatory poly(l-glutamic acid) composite hydrogels containing metal ion-coordinated nanoparticles for effective hemostasis and wound healing

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