Assessing an intervention to increase knowledge related to cervical cancer and the HPV vaccine

Ford, Marvella E.; Cannady, Kimberly; Nahhas, Georges J.; Knight, Kenneth R.; Chavis, Courtney; Crawford, Brittney; Malek, Angela M.; Martino, Erica; Frazier, Starr; Gathers, Antiqua; Lawton, Claudia; Cartmell, Kathleen B.; Luque, John S.

doi:10.1016/bs.acr.2020.01.007

Cited by 8 publications

(16 citation statements)

References 23 publications

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“…It induces epithelial cells' malignant transformation and suppresses the immune response via encoding oncoproteins [17]. For instance, E6 and E7 proteins encoded by HRHPV could promote cervical cancer [18,19], E6 protein encourages the growth of cervical cancer cells by targeting P53 protein [20], and E7 protein immortalizes human epithelial cells by targeting pRb protein [21]. Rapid proliferation is a driving force for the massive energy required by malignant cells to adapt to metabolic modi cations [22].…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Plasma-based Endogenous Metabolomics Profiling of High-Risk Human Papillomavirus and Their Emerging Roles in the Progression of Cervical Cancer

Wang

Chen

et al. 2021

Preprint

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Objective: High-risk human papillomavirus (HR-HPV) is the main etiological factor for cervical cancer. Accumulating evidence has suggested that the active role of metabolites in the initiation and progression of cancers. This study was to explore the metabolic profiles of HR-HPV infection and their potential functions in cervical cancer.Methods: Non-targeted metabolomics approach was used to detect metabolic alterations in the plasma obtained from HPV-16 positive (HPV16 (+)), HPV-18 positive (HPV18 (+)) and HPV negative (CTL) individuals, followed by CCK8 experiment to detect the effect of different metabolites on the proliferation of Hela and GH354. A cell migration test then verified significant metabolites on the migration of Hela and GH354. Q RT-qPCR and western blot were used to detect malignant progression related mRNA and protein expression levels of cervical cancer.Results: HR-HPV groups shared 24 dysregulated metabolites (such as amino acids, ceramides, glycerophosphocholines). Further experiments showed ceramide species, including C8 inhibits cervical cancer cells proliferation and migration in vitro. In contrast, C12 significantly enhanced cervical cancer cells proliferation and migration in vitro. Protein and mRNA expressions indicated C8 and C12 were related to the malignant behavior of cervical cancer in vitro. The underlying mechanism demonstrated that C8 intervention inhibited proliferation and migration in cervical cancer cells via the MAPK/JNK signaling pathway, while C12 intervention promoted proliferation and migration in cervical cancer cells via the MAPK/ERK signaling pathway. These findings may contribute to the treatment of HR-HPV-induced cervical cancer by intervening in its initiation and progression.Conclusion: Our study shed some light on how metabolites influenced the relationship between HR-HPV oncogenic capability and metabolic phenotype change and identify species C8 and C12 as critical lipid metabolites that modulate cervical cancer cell’s function.

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Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Plasma-based Endogenous Metabolomics Profiling of High-Risk Human Papillomavirus and Their Emerging Roles in the Progression of Cervical Cancer

Wang

Chen

et al. 2021

Preprint

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“…Eight of the 79 studies (10.1%) included a large proportion of parents from diverse racial and ethnic identities (de ned ≥ 50% other races than White), 59,75,77,78,80−83 6 (7.6%) included adolescents from diverse groups, 23,30,45,46,51,58 8 (10.1%) included both parents and children from diverse groups, 36,37,42,44,52,54,55,64 6 (7.6%) included young adults from diverse groups, 28,41,62,67,73,94 and 1 included both young adults and children from diverse groups (1.3%). 27 Socio-ecological Levels.…”

Section: Resultsmentioning

confidence: 99%

“…Twenty-seven interventions (33.8%) reported the use of theory in intervention development. 25,[28][29][30]34,35,38,39,42,44,49,51,52,[59][60][61]65,68,70,77,80,81,83,84,86,92,100 Theories or frameworks referenced included the Elaboration Likelihood Model, Culture-centric narrative theory, Health Belief Model, Theory of Reasoned Action/Planned Behavior, Moral Norm and Social Cognitive Theory.…”

Section: Resultsmentioning

confidence: 99%

“…Other components included patient reminders (13, 16.5%), 23,35,36,43,44,51,54,70,71,74,80,93,98 improving access to the HPV vaccine (6, 7.6%), 24,40,45,52,53,71 health systems change (6, 7.6%), 31,48,52,58,83,98 incentives (4, 5.1%), 33,43,47,56 and community-wide campaigns or outreach (3, 3.8%). 26,52,77 Some studies combined two components (29, 36.7%), [23][24][25][26][27][28]30,32,35,40,46,49,53,54,58,61,63,65,70,73,75,…”

Section: Intervention Components Varied From Education To Offering Va...mentioning

confidence: 99%

“…Facilitators and barriers to intervention implementation. Several studies reported facilitators (13 studies, 16.5%) 23,24,26,29,30,34,44,46,62,65,77,88,89 and barriers (22 studies, 27.58%) 23,[29][30][31][32]35,42,47,50,51,58,61,62,71,72,74,82,88,89,92,95,98 to intervention implementation. Facilitators included use of patient navigators and user-friendly resources, 23,29,62 interactive information sessions, 24,88,89 low cost interventions, 30,65,88 and quality improvement initiatives.…”

Section: Intervention Components Varied From Education To Offering Va...mentioning

confidence: 99%

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A Systematic Review of Interventions to promote HPV Vaccination Globally

Escoffery

Petagna

Agnone

et al. 2022

Preprint

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Background Despite the human papillomavirus (HPV) vaccine being a safe, effective cancer prevention method, its uptake is subomptimal in the United States (U.S.). Previous research have found a variety of intervention strategies (environmental and behavioral) in increasing its uptake. The purpose of the study is to systematically review the literature for interventions to promote HPV vaccination from 2015 to 2020. Methods We updated a systemative review of interventions to promote HPV vaccine uptake globally. We ran key word searches in six bibliographic databases. Audiences, design, level of intervention, components and outcomes were abstracted from the full-text articles in Excel databases. Results Of the 79 articles, most were conducted in the U.S. (72.2%) and in clinical (40.5%) or school settings (32.9%), and were directed a single level (76.3%). Related to the intervention type, most were informational (n = 25, 31.6%) or patient-targeted decision support (n = 23, 29.1%). About 24% were multi-level interventions, with 16 (88.9%) combining two levels. Twenty-seven (33.8%) reported the use of theory in intervention development. Of those reporting HPV vaccine outcomes, post-intervention vaccine initiation ranged from 5–99.2%, while series completion ranged from 6.8–93.0%. Facilitators to implementation were use of patient navigators and user-friendly resources, while barriers included costs, time to implement and difficulties of integrating interventions into organizational workflow. Conclusions There is a strong need to expand implementation of HPV-vaccine promotion interventions beyond education alone and at a single level of intervention. Development and evaluation of effective strategies and multi-level interventions may increase the uptake of the HPV vaccine among adolescents and young adults.

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Plasma-Based Metabolomics Profiling of High-Risk Human Papillomavirus and their Emerging Roles in the Progression of Cervical Cancer

Chen

et al. 2022

BioMed Research International

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High-risk human papillomavirus (HR-HPV) is the main etiological factor for cervical cancer. Accumulating evidence has suggested the active role of metabolites in the initiation and progression of cancers. This study explored the plasma metabolic profiles of HPV-16 positive (HPV16 (+)), HPV-18 positive (HPV18 (+)), and HPV negative (CTL) individuals using a nontargeted metabolomics approach. C8 ceramide-1-Phosphate (d18 : 1/8 : 0) was found to inhibit cervical cancer cell proliferation and migration in vitro, evidenced by CCK8 experiments, a cell migration test, RT-qPCR, and western blotting. The underlying mechanism demonstrated that C8 inhibited proliferation and migration in cervical cancer cells via the MAPK/JNK1 signaling pathway. These findings may contribute to the clinical treatment of HR-HPV-induced cervical cancer by intervening in its initiation and progression.

show abstract

Assessing an intervention to increase knowledge related to cervical cancer and the HPV vaccine

Cited by 8 publications

References 23 publications

WITHDRAWN: Plasma-based Endogenous Metabolomics Profiling of High-Risk Human Papillomavirus and Their Emerging Roles in the Progression of Cervical Cancer

WITHDRAWN: Plasma-based Endogenous Metabolomics Profiling of High-Risk Human Papillomavirus and Their Emerging Roles in the Progression of Cervical Cancer

A Systematic Review of Interventions to promote HPV Vaccination Globally

Plasma-Based Metabolomics Profiling of High-Risk Human Papillomavirus and their Emerging Roles in the Progression of Cervical Cancer

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