Assessing amino acid utilization in young Nellore steers fed high-concentrate diets with different sources and levels of nitrogen

Souza, Vinícius Carneiro de; Messana, Juliana Duarte; Batista, Erick Darlisson; Alves, Kênia Larissa Gomes Carvalho; Dias, Ana Veronica Lino; Campos, L.M.; Lima, Lais de Oliveira; Granja-Salcedo, Yury Tatiana; Faria, Letícia Rodrigues; Carvalho, Geovany Macêdo; Pires, Alexandre Vaz; Berchielli, Telma Teresinha

doi:10.1016/j.anifeedsci.2020.114642

Cited by 3 publications

(5 citation statements)

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“…The results of this study differ from those observed by Souza et al [ 10 ], who found that feeding cattle with different protein sources resulted in a distinct AA profile in microbial protein. NPN, being readily available in the rumen, tends to increase microbial protein synthesis, leading to higher concentrations of AA from microbial origin that reach the intestine.…”

Section: Discussioncontrasting

confidence: 99%

“…The authors also recommended that ruminal N status could be improved by incorporating a component of ruminal protein degradability into prediction models. However, the results regarding AA flow and digestibility can be utilized to develop future feed formulation models that consider the variation between different diets and specific coefficient factors for AA digestibility and the efficiency of individual utilization under specific conditions [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…In turn, RUP supplementation is known to increase AA flow to the SI, and its use by animals is not associated with the energy available in the rumen. Studies have demonstrated increases in N retention, N recycling, and animal performance when the dietary rumen degradable protein (RDP) concentration is low [ 7 , 9 , 10 ]. AA supply to ruminants is dependent on microbial protein synthesis in the rumen, the protein that escapes ruminal degradation (RUP) and reaches the SI, and the profile and digestibility of AA [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…AA supply to ruminants is dependent on microbial protein synthesis in the rumen, the protein that escapes ruminal degradation (RUP) and reaches the SI, and the profile and digestibility of AA [ 11 , 12 ]. However, the efficiency of N utilization is dependent on the profile and digestibility of AA in the SI, which is known to be affected by diet [ 10 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, such information may contribute to improving the prediction of N use efficiency, which is scarce in the literature on beef cattle grazing low-quality forage. Furthermore, the models used in ruminant nutrition, such as NRC [19], BR-Corte [20], and NASEM [21], consider that the AA profiles of feed and RUP are similar, as well as the digestibility of individual AA, which is not true [10].…”

Section: Introductionmentioning

confidence: 99%

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Efficiency of Amino Acid Utilization in Nellore Cattle Grazing Low-Quality Forage Supplemented with Different Sources of Nitrogen

Dias,

Messana,

Granja-Salcedo

et al. 2023

Life

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This study aimed to evaluate the effects of supplementation with non-protein nitrogen (NPN) or ruminal undegradable protein (RUP) on intake, digestibility, and amino acid (AA) use efficiency of Nellore cattle grazing during the dry season. Eight Nellore steers (12 ± 2 months old) were used in quadruplicate Latin squares (2 × 2). The animals were placed on Urochloa brizantha cv. Xaraés under continuous grazing. The treatments included the following: (1) urea supplementation (NPN) and (2) supplementation of corn gluten meal 60 (CGM, RUP). Animals supplemented with CGM showed higher intakes of dry matter (DM) supplement, total AA, essential AA, and individual AA. The supplementation did not affect the total AA digestibility, total AA flux, and the AA fluxes of microbial origin and RUP from the diet (p > 0.05). The ruminal microorganism origin flux of total AA to the duodenum was 44.5% and 52.7% for animals supplemented with NPN and CGM, respectively. Animals supplemented with CGM showed an increase in blood concentrations of isoleucine (+19.09 μmol/L), cystine (+27.29 μmol/L), and albumin (+0.11 g/dL) (p < 0.05), but this increase was not accompanied by an improvement in N use efficiency of steers (p > 0.05). RUP supplementation via CGM can be an efficient nutritional strategy to enhance the intake and absorption of AA by Nellore cattle grazing low-quality forage during the dry season.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Efficiency of Amino Acid Utilization in Nellore Cattle Grazing Low-Quality Forage Supplemented with Different Sources of Nitrogen

Dias,

Messana,

Granja-Salcedo

et al. 2023

Life

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Ruminal tissue uptake of amino acids in Holstein cows when supply of nutrients within the rumen differs

Souza¹,

Aguilar²,

Storm³

et al. 2023

animal

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Effects of high concentrate rations on ruminal fermentation and microbiota of yaks

et al. 2022

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Ruminal microflora is closely correlated with the ruminant’s diet. However, information regarding the effect of high concentrate diets on rumen microflora in yaks is lacking. In the current study, 24 healthy male yaks were randomly assigned to two groups, each fed with different diets: less concentrate (LC; concentrate: coarse = 40: 60) and high concentrate (HC; concentrate: coarse = 80: 20) diets. Subsequently, a 21-day feeding trial was performed with the yaks, and rumen fluid samples were collected and compared using 16 s rRNA sequencing. The results showed that NH3-N, total VFA, acetate, butyrate, isobutyrate, and isovalerate were significantly higher in the HC group than that in the LC group (p < 0.05), while microbial diversity and richness were significantly lower in the HC group (p < 0.05). Principal coordinate analysis indicated that rumen microflora was significantly different in LC and HC groups (p < 0.05). In the rumen, phyla Firmicutes and Bacteroidota were the most abundant bacteria, with Firmicutes being more abundant, and Bacteroidota being less abundant in the HC group than those found in the LC group. Christensenellaceae_R-7_group and Prevotella are the highest abundant ones at the genus level. The relative abundance of Acetitomaculum, Ruminococcus, and Candidatus_Saccharimonas were significantly higher in the HC group than that in the LC group (p < 0.05), while the relative abundance of Olsenella was significantly lower in the HC group than in the LC group (p < 0.05). Compared to the LC group, the relative abundance of Prevotella, Ruminococcus, and Candidatus_Saccharimonas was significantly higher in the HC group. The relative abundances of Prevotella, Prevotellaceae_UCG-003, Olsenella, Ruminococcus, Acetitomaculum, Candidatus_Saccharimonas, and NK4A214_group were correlated with ruminal fermentation parameters (p < 0.05). Furthermore, PICRUSt 2 estimation indicated that microbial genes associated with valine, leucine, and isoleucine biosynthesis were overexpressed in the rumen microflora of yaks in the HC group (p < 0.05). Conclusively, our results suggest that high concentrate diets affect the microflora composition and fermentation function in yak rumen. The present findings would provide new insights into the health of yaks under high concentrate feeding conditions and serve as a potent reference for the short-term fattening processes of yaks.

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Assessing amino acid utilization in young Nellore steers fed high-concentrate diets with different sources and levels of nitrogen

Cited by 3 publications

References 61 publications

Efficiency of Amino Acid Utilization in Nellore Cattle Grazing Low-Quality Forage Supplemented with Different Sources of Nitrogen

Efficiency of Amino Acid Utilization in Nellore Cattle Grazing Low-Quality Forage Supplemented with Different Sources of Nitrogen

Ruminal tissue uptake of amino acids in Holstein cows when supply of nutrients within the rumen differs

Effects of high concentrate rations on ruminal fermentation and microbiota of yaks

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