Assessing a novel room temperature DNA storage medium for forensic biological samples

Lee, Steven B.; Clabaugh, Kimberly; Silva, Brie; Odigie, Kingsley O.; Coble, Michael D.; Loreille, Odile; Scheible, Melissa; Fourney, Ron M.; Stevens, Jesse; Carmody, George R.; Parsons, Thomas J.; Pozder, Arijana; Eisenberg, Arthur J.; Budowle, Bruce; Ahmad, Taha; Miller, Russell; Crouse, Cecelia A.

doi:10.1016/j.fsigen.2011.01.008

Cited by 49 publications

(34 citation statements)

References 24 publications

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“…Commercial room-temperature storage solutions (e.g. those from Biomatrica Inc.) may provide attractive options for sample storage due to lower energy requirements, reduced likelihood of catastrophic failure, and reportedly better sample stability (Lee et al 2011). …”

Section: Extraction Of Dna From Plant Tissuementioning

confidence: 99%

Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples

Lear¹,

Dickie²,

Banks³

et al. 2018

108

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Advances in the sequencing of DNA extracted from media such as soil and water offer huge opportunities for biodiversity monitoring and assessment, particularly where the collection or identification of whole organisms is impractical. However, there are myriad methods for the extraction, storage, amplification and sequencing of DNA from environmental samples. To help overcome potential biases that may impede the effective comparison of biodiversity data collected by different researchers, we propose a standardised set of procedures for use on different taxa and sample media, largely based on recent trends in their use. Our recommendations describe important steps for sample pre-processing and include the use of (a) Qiagen DNeasy PowerSoil ® and PowerMax ® kits for extraction of DNA from soil, sediment, faeces and leaf litter; (b) DNeasy PowerSoil ® for extraction of DNA from plant tissue; (c) DNeasy Blood and Tissue kits for extraction of DNA from animal tissue; (d) DNeasy Blood and Tissue kits for extraction of DNA from macroorganisms in water and ice; and (e) DNeasy PowerWater ® kits for extraction of DNA from microorganisms in water and ice. Based on key parameters, including the specificity and inclusivity of the primers for the target sequence, we recommend the use of the following primer pairs to amplify DNA for analysis by Illumina MiSeq DNA sequencing: (a) 515f and 806RB to target bacterial 16S rRNA genes (including regions V3 and V4); (b) #3 and #5RC to target eukaryote 18S rRNA genes (including regions V7 and V8); (c) #3 and #5RC are also recommended for the routine analysis of protist community DNA; (d) ITS6F and ITS7R to target the chromistan ITS1 internal transcribed spacer region; (e) S2F and S3R to target the ITS2 internal transcribed spacer in terrestrial plants; (f) fITS7 or gITS7, and ITS4 to target the fungal ITS2 region; (g) NS31 and AML2 to target glomeromycota 18S rRNA genes; and (h) mICOIintF and jgHCO2198 to target cytochrome c oxidase subunit I (COI) genes in animals. More research is currently required to confirm primers suitable for the selective amplification of DNA from specific vertebrate taxa such as fish. Combined, these recommendations represent a framework for efficient, comprehensive and robust DNA-based investigations of biodiversity, applicable to most taxa and ecosystems. The adoption of standardised protocols for biodiversity assessment and monitoring using DNA extracted from environmental samples will enable more informative comparisons among datasets, generating significant benefits for ecological science and biosecurity applications.

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Section: Extraction Of Dna From Plant Tissuementioning

confidence: 99%

Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples

Lear¹,

Dickie²,

Banks³

et al. 2018

108

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“…STRs can be classified according on their structures as simple, compound, complex and complex highly variable. 11 ( See Fig. 4).…”

Section: Short Tandem Repeats (Strs)mentioning

confidence: 99%

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Al-Kandari¹,

Singh²,

Sangar³

2016

IJPSR

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Forensic science is growing rapidly in the world today. During the past ten years, medico-legal investigations highly expanded to include all areas of forensic science. The present aim of project investigated 29 samples of blood and 29 samples of saliva swabs that were collected from human volunteers. The saliva samples were collected by buccal swab but the blood samples were collected by Bode Secur Swab S.I.T. Collector. The experiments were done at four different temperatures (55°C, 37°C, 24°C and 4°C) for 28 days. The results showed that, DNA quantity that was investigated at a temperature of 4°C and 24°C in both blood and saliva samples was more or less remained the same during the whole period of the study, comparing values for day one with all other days including day 28. DNA quantification of human blood following extraction at 37°C was 46.14 ng/μl ± 0.22 at day one then starts to decrease until it reached 36.05 ng/μl ± 0.07 at day 28. In contrast, the result obtained from real-time PCR showed that, when the temperature was raised to 55°C, the DNA started to degrade with time until it reaches zero at day 12. The results clearly show that DNA is extremely sensitive to heat. In conclusion, the present project has shown that accidental deaths are the major cause for un-natural deaths in Kuwait. Moreover, the study concluded that an environmental temperature of 55°C, revealed no DNA survival after 12 days of exposure.

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“…The interest in preserving DNA as dried preparations has increased vastly in response to the growing demand from a variety of fields, including pharmaceutical sciences [56,82,91,113,121,122], forensics [123], molecular diagnostics [124], and biorepository management [125]) for storing DNA samples or tumor specimen banks [114,126] for prolonged periods of time, sometimes in large numbers, in regions where sophisticated storage equipment may not be available. Although the requirements are quite different in each of these fields, the need for strategies that provide highly stable storage conditions (e.g., room temperature storage) seems to be a common factor.…”

Section: Preservation Of Dnamentioning

confidence: 99%

Stabilization of Plasmid DNA and Lipid-Based Therapeutics as Dehydrated Formulations

Molina

Payton

Anchordoquy

2015

Lyophilized Biologics and Vaccines

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Assessing a novel room temperature DNA storage medium for forensic biological samples

Cited by 49 publications

References 24 publications

Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples

Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples

Untitled

Stabilization of Plasmid DNA and Lipid-Based Therapeutics as Dehydrated Formulations

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