Assesment of metal inhibition of antioxidant enzyme glutathione reductase from rainbow trout liver

Simple methanesulfonates are hydrolyzed by the sulfatase carbonic anhydrase activity

“…However the binding site of 4-NPA itself is unknown, but it is presumed to be in the same region as that of CO 2 , the physiological substrate of this enzyme [9][10][11] . Similarly to salicylic acid derivatives and phenolic compounds investigated earlier by us 8,[26][27][28][29][30][31][32][33] , the investigated compounds act as competitive inhibitors with 4-NPA as substrate, that is, they bind in same regions of the active site cavity as compared to the substrate. 2.…”

“…It is known that CA has been purified many times from different organisms and the effects of various chemicals, pesticides, anions, metal ions and drugs have been investigated on its activity [7][8][9][10][11][12][13][14][15][16][17][29][30][31][32][33] . In this study, CA I, II, IV and VI were purified from human erythrocytes and serum by a simple procedure using Sephrarose-4B-aniline-sulfanilamide affinity column.…”

“…hCA IV was purified, 84.7-fold with a specific activity of 742 EUmg −1 and overall yield of 17.5%. Similarly, hCA VI was purified, 69.2-fold with a specific activity of 418 EUmg −1 and overall yield of 13.2% [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] . We report here a study on the inhibitory effects of methanesulfonate derivatives and some phenolic compounds on the CA esterase activity of isoforms hCA I, II, IV and VI.…”

Susceptibility of cord blood antioxidant enzymes glutathione reductase, glutathione peroxidase and glutathione S-transferase to different antibiotics:in vitroapproach

“…Glutathione S-transferases (GSTs) detoxify such endogenously produced electrophiles by conjugation with glutathione (GSH) as well as by acting as glutathione peroxidases to detoxify toxic base propenals like thymidine hydroperoxide 29 . Particularly, GR is essential for the maintenance of cellular glutathione in its reduced form, which is highly nucleophilic for many reactive electrophils 30,31 . Numerous studies have demonstrated that oxidative stress is a key pathogenic factor in the development of illness complications.…”

PADRONIZAÇÃO DA QUANTIFICAÇÃO DE GLUTATIONA REDUTASE EM Aegla singularis (ANOMURA, CRUSTACEA) UTILIZANDO PLANEJAMENTO EXPERIMENTAL DCCR

“…37,38 A maior contaminação por metais provenientes de atividades antrópicas poderia explicar a menor atividade da GR nas BH dos Rios Suzana e Ligeirinho-Leãozinho, considerando que já foi demonstrado que a GR pode ser inibida por diferentes tipos de metais. 39 Cossu et al 40 realizaram um estudo in situ com bivalves da espécie Unio tumidus e observaram redução de atividade da GR nos organismos coletados em trechos de riachos contaminados por misturas de diversos xenobióticos, incluindo alta concentração de metais como cromo, cobre, chumbo e mercúrio. Investigações adicionais são necessárias para confirmar se o padrão de redução da GR em Aegla está associado efetivamente ao nível de metais no ambiente de BH ou a outros fatores que afetam a qualidade da água nestes locais.…”