Assesment of artificial caries lesions through scanning electron microscopy and cross-sectional microhardness test

Marsillac, Mirian de Waele Souchois de; Vieira, Ricardo Cardoso

doi:10.4103/0970-9290.116699

Cited by 12 publications

(6 citation statements)

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“…In initial carious lesions, the relatively intact surface layer generally exhibits a clearer perikymata pattern and in numerous studies so-called focal holes have been seen [32] . Using scanning electromicroscopy, de Marsillac et al found that micro-sized diffusion pathways through intercrystalline and interprismatic spaces could be detected on the surface of carious enamel [33] . On the other hand, our previous investigation has revealed that CS-AMEL hydrogel works through the nano-sized amelogenin-Ca-P clusters that eventually evolve into enamel-like co-aligned crystals [9] .…”

Section: Discussionmentioning

confidence: 99%

Efficacy of amelogenin-chitosan hydrogel in biomimetic repair of human enamel in pH-cycling systems

Ruan

Liberman

Bapat

et al. 2015

JBEI

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Amelogenin-chitosan (CS-AMEL) hydrogel has shown great potential for the prevention, restoration, and treatment of defective enamel. As a step prior to clinical trials, this study aimed to examine the efficacy of CS-AMEL hydrogel in biomimetic repair of human enamel with erosive or caries-like lesions in pH-cycling systems. Two models for enamel defects, erosion and early caries, were addressed in this study. Two pH-cycling systems were designed to simulate the daily cariogenic challenge as well as the nocturnal pH conditions in the oral cavity. After pH cycling and treatment with CS-AMEL hydrogel, a synthetic layer composed of oriented apatite crystals was formed on the eroded enamel surface. CS-AMEL repaired the artificial incipient caries by re-growing oriented crystals and reducing the depth of the lesions by up to 70% in the pH-cycling systems. The results clearly demonstrate that the CS-AMEL hydrogel is effective at the restoration of erosive and carious lesions under pH-cycling conditions.

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Section: Discussionmentioning

confidence: 99%

Efficacy of amelogenin-chitosan hydrogel in biomimetic repair of human enamel in pH-cycling systems

Ruan

Liberman

Bapat

et al. 2015

JBEI

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“…Despite the surface of the initial carious lesions remaining relatively intact, nano-sized AMEL-CaP clusters can permeate the deep layer through micro-sized diffusion pathways in the inter-crystalline and inter-prismatic space [ 42 , 43 ]. In situ biomineralisation induced by chitosan (CS)-AMEL was found to result in the formation of a dense interface between the newly grown layer and natural enamel, such that the mechanical properties of the enamel-like layer improved compared to those of etched enamel (Table 1 ) [ 44 ].…”

Section: Biomimetic Systems For Enamel Restorationmentioning

confidence: 99%

Biomimetic mineralisation systems for in situ enamel restoration inspired by amelogenesis

Wang

Liu

Ren

et al. 2021

J Mater Sci: Mater Med

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Caries and dental erosion are common oral diseases. Traditional treatments involve the mechanical removal of decay and filling but these methods are not suitable for cases involving large-scale enamel erosion, such as hypoplasia. To develop a noninvasive treatment, promoting remineralisation in the early stage of caries is of considerable clinical significance. Therefore, biomimetic mineralisation is an ideal approach for restoring enamel. Biomimetic mineralisation forms a new mineral layer that is tightly attached to the surface of the enamel. This review details the state-of-art achievements on the application of amelogenin and non-amelogenin, amorphous calcium phosphate, ions flow and other techniques in the biomimetic mineralisation of enamel. The ultimate goal of this review was to shed light on the requirements for enamel biomineralisation. Hence, herein, we summarise two strategies of biological minimisation systems for in situ enamel restoration inspired by amelogenesis that have been developed in recent years and compare their advantages and disadvantages.

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“…A detailed understanding and quantification of the involved processes will allow for deriving alternative tooth repair procedures. The caries lesions can be investigated ex vivo taking advantage of a wide variety of methods including microradiography [ 2 ], light microscopy [ 3 ], electron microscopy [ 3 , 4 ], confocal laser scanning microscopy [ 5 ], atomic force microscopy [ 6 ], and nano-indentation [ 7 ]. Some of these techniques require destructive tissue preparation or just yield a two-dimensional snapshot of the three-dimensional lesion at a particular stage during disease development.…”

Section: Introductionmentioning

confidence: 99%

Mineralization of Early Stage Carious Lesions In Vitro—A Quantitative Approach

et al. 2015

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Micro computed tomography has been combined with dedicated data analysis for the in vitro quantification of sub-surface enamel lesion mineralization. Two artificial white spot lesions, generated on a human molar crown in vitro, were examined. One lesion was treated with a self-assembling peptide intended to trigger nucleation of hydroxyapatite crystals. We non-destructively determined the local X-ray attenuation within the specimens before and after treatment. The three-dimensional data was rigidly registered. Three interpolation methods, i.e., nearest neighbor, tri-linear, and tri-cubic interpolation were evaluated. The mineralization of the affected regions was quantified via joint histogram analysis, i.e., a voxel-by-voxel comparison of the tomography data before and after mineralization. After ten days incubation, the mean mineralization coefficient reached 35.5% for the peptide-treated specimen compared to 11.5% for the control. This pilot study does not give any evidence for the efficacy of peptide treatment nor allows estimating the necessary number of specimens to achieve significance, but shows a sound methodological approach on the basis of the joint histogram analysis.

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Assesment of artificial caries lesions through scanning electron microscopy and cross-sectional microhardness test

Abstract: This methodology can be used to induce AAWSLs in human dental enamel but surface erosion has to be taken into account when performing CSMH test.

Cited by 12 publications

References 0 publications

Efficacy of amelogenin-chitosan hydrogel in biomimetic repair of human enamel in pH-cycling systems

Efficacy of amelogenin-chitosan hydrogel in biomimetic repair of human enamel in pH-cycling systems

Biomimetic mineralisation systems for in situ enamel restoration inspired by amelogenesis

Mineralization of Early Stage Carious Lesions In Vitro—A Quantitative Approach

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