Assembly of osteoblastic cell micro-arrays on diamond guided by protein pre-adsorption

Rezek, Bohuslav; Ukraintsev, Egor; Kromka, Alexander; Ledinský, Martin; Brož, Antonín; Nosková, Lenka; Hartmannová, Hana; Kalbáčová, Marie Hubálek

doi:10.1016/j.diamond.2009.09.016

Cited by 19 publications

(23 citation statements)

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“…We used the SAOS‐2 osteoblast cell line because one of the possible utilization of the NCD surface is on dental and orthopedic implants. We elucidate the process of differential cell adhesion on NCD‐H and NCD‐O surfaces (topographically identical surfaces varying only in topmost surface atoms) that was observed in our previous studies but remained not fully understood. We discuss the role of the cell adhesion during plating and active cell migration after the adhesion.…”

Section: Introductionmentioning

confidence: 87%

See 1 more Smart Citation

Osteoblast adhesion, migration, and proliferation variations on chemically patterned nanocrystalline diamond films evaluated by live‐cell imaging

Brož

Ukraintsev

Kromka

et al. 2017

J Biomedical Materials Res

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Cell fate modulation by adapting the surface of a biocompatible material is nowadays a challenge in implantology, tissue engineering as well as in construction of biosensors. Nanocrystalline diamond (NCD) thin films are considered promising in these fields due to their extraordinary physical and chemical properties and diverse ways in which they can be modified structurally and chemically. The initial cell distribution, the rate of cell adhesion, distance of cell migration and also the cell proliferation are influenced by the NCD surface termination. Here, we use real-time live-cell imaging to investigate the above-mentioned processes on oxidized NCD (NCD-O) and hydrogenated NCD (NCD-H) to elucidate cell preference to the NCD-O especially on surfaces with microscopic surface termination patterns. Cells adhere more slowly and migrate farther on NCD-H than on NCD-O. Cells seeded with a fetal bovine serum (FBS) supplement in the medium move across the surface prior to adhesion. In the absence of FBS, the cells adhere immediately, but still exhibit different migration and proliferation on NCD-O/H regions. We discuss the impact of these effects on the formation of cell arrays on micropatterned NCD. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1469-1478, 2017.

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Section: Introductionmentioning

confidence: 87%

“…Our previous studies indicated that the protein layers formed early during cultivation in an FBS‐supplemented medium. The protein layers on NCD‐O and on NCD‐H were different in thickness and in adhesion force . This indicates a difference in the composition of the layer.…”

Section: Introductionmentioning

confidence: 99%

Osteoblast adhesion, migration, and proliferation variations on chemically patterned nanocrystalline diamond films evaluated by live‐cell imaging

Brož

Ukraintsev

Kromka

et al. 2017

J Biomedical Materials Res

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show abstract

“…It was proposed that the selectivity is due to the serum proteins, which are adsorbed in about the same monolayer thickness (2-4 nm) on both H and O-diamond surfaces, but in different composition and conformations of proteins [88]. When osteoblasts were placed on the diamond surface in McCoy's 5A medium without FBS, cell attachment on H/O-patterned diamond surfaces was not selective [85,89]. This excluded a direct effect of diamond C-H and C-O surface dipoles on the cell selectivity.…”

Section: Molecular Mechanisms Of Signaling Transduction From Uncd Filmentioning

confidence: 99%

Nanocrystalline Diamond Films: Applications and Advances in Nanomedicine

Chen¹,

Lee²,

Chiu³

2011

Biomaterials Applications for Nanomedicine

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“…As our substrate of choice for biological studies, we use the monocrystalline diamond (MCD) as it is a well-defined and chemically tailorable substrate Rezek et al, 2010).…”

Section: Methodsmentioning

confidence: 99%