Assembly of Anionic Conjugated Polymer with 6‐<i>O</i>‐Modified PNP‐<i>β</i>‐Galactoside for Fluorescence Logic‐signal‐based Multiplex Detections of Enzymes

Wang, Yajuan; Xin, Bao-juan; Duan, Xinrui; Xing, Guo‐wen; Wang, Shu

doi:10.1002/marc.201000165

Cited by 10 publications

(8 citation statements)

References 49 publications

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“…Enzymes and coenzymes have been extensively used in sensing system owing to their high activity and specificity, acting as biosensors in the electrochemical and optical area [ 15 , 16 ]. They are capable of changing function and structure, thus frequently modified onto the sensor or added in the solution to accelerate or change the process [ 17 , 18 ]. Compared with the electrochemical methods, optical methods are more reliable in low concentration analyte detection using the UV and fluorescence spectra.…”

Section: Introductionmentioning

confidence: 99%

Graphene Quantum Dots and Enzyme-Coupled Biosensor for Highly Sensitive Determination of Hydrogen Peroxide and Glucose

Wang

Shen

Huang

et al. 2018

IJMS

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In this paper, a simple and specific graphene quantum dots (GQDs)-based fluorescent biosensor adopted for the determination of glucose based on the combination of the enzyme-coupled method and fluorescence quenching mechanism is demonstrated. Glucose was oxidized by the enzyme glucose oxidase (GOx), forming hydrogen peroxide (H2O2) via the catalysis by horseradish peroxidase (HRP). H2O2 was then employed to oxidize phenol to quinone, which led to effective quenching effect in the GQDs–GOx–HRP–phenol system. By optimizing the reaction conditions of the GQDs-enzyme system, a linear relationship between the concentration of glucose and the fluorescence intensity over a range of 0.2–10 μmol/L was obtained. The limit of detection for glucose is 0.08 μmol/L. The present biosensor for the determination of glucose showed satisfactory reproducibility and accuracy in human serum samples. Since the enzymes have high specificity and unique affinity to the certain substance, the enzyme-coupled system promises a sensitive way for further detection of those chemicals which could be oxidized by enzymes and generated H2O2 or glucose. GQDs and other fluorescent materials coupled with several enzymes can be applied to extensive sensing field.

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Section: Introductionmentioning

confidence: 99%

Graphene Quantum Dots and Enzyme-Coupled Biosensor for Highly Sensitive Determination of Hydrogen Peroxide and Glucose

Wang

Shen

Huang

et al. 2018

IJMS

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“…When the GGT is added into the mixture of GPNA and glycylglycine (Gly‐Gly) in buffer, glutaminyl group will be transferred to Gly‐Gly and p‐nitroaniline (PNA) will be released. Although the polymer PFPBOH contains hydrophilic cationic quaternary ammonium moieties and has good water solubility, the hydrophobic backbone of polyfluorene still can interact with PNA through hydrophobic interactions 22. Since PNA is able to effectively quench the fluorescence of PFPBOH, GGT can be detected through the decrease of fluorescence.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of a Bifunctional Fluorescent Polymer for Cell Imaging and Enzyme Detection

Wen

Zhu

Liu

et al. 2012

Macro Chemistry & Physics

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A new bifunctional cationic conjugated polyfluorene derivative (PFPBOH) containing phenylboronic acid group has been synthesized and characterized. The phenylfluorenyl backbone and positively charged groups in the side chain give the polymer PFPBOH excellent fluorescence property and good water solubility. Phenylboronic acid moieties on the side chain of the polymer form cyclic esters with adjacent diols on cell membrane, which can be employed in cell imaging. Besides that, the fluorescence of PFPBOH can be quenched by p‐nitroaniline released via enzyme reaction, thus, the polymer can be used for γ‐glutamyltranspeptidase detection through a fluorescence “turn off” mechanism. These findings exhibit great potential for developing multifunctional polymer materials for simultaneous imaging and detection.

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“…Up to now, it is unclear on how to use water‐soluble CPEs to realize the detection of sialidase. On the basis of our previous studies on enzyme assay16 and sialic acid chemistry,17 we report herein a new continuous fluorometric method to measure sialidase activity and its inhibitor by exploiting the interaction of PNP‐Neu5 Ac with CPEs.…”

Section: Methodsmentioning

confidence: 99%

Separation of Time‐Resolved Phenomena in Surface‐Enhanced Raman Scattering of the Photocatalytic Reduction of p‐Nitrothiophenol

Lantman

Peinder²,

Mank

et al. 2015

ChemPhysChem

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Im Rahmen des Projekts Clean Sky wurde ein Ecodesign Guideline Tool für die Luftfahrtindustrie entwickelt. Entsprechend den Zielen des europäischen Beratungsgremiums für die Luft‐ und Raumfahrt (ACARE) wurden zahlreiche luftfahrtspezifische Life Cycle Assessment Daten zu Materialien und Materialkombinationen gesammelt und in ein neu entwickeltes, einfach handhabbares Datenbanktool implementiert. Somit bietet das Tool eine robuste Grundlage für ein drittes Entscheidungskriterium neben Kosten und Gewicht. Das Tool ist auf die End of Life Phase fokussiert, insb. auf Wirtschaftlichkeit, Separation und Recyclingprozesse.

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Assembly of Anionic Conjugated Polymer with 6‐O‐Modified PNP‐β‐Galactoside for Fluorescence Logic‐signal‐based Multiplex Detections of Enzymes

Cited by 10 publications

References 49 publications

Graphene Quantum Dots and Enzyme-Coupled Biosensor for Highly Sensitive Determination of Hydrogen Peroxide and Glucose

Graphene Quantum Dots and Enzyme-Coupled Biosensor for Highly Sensitive Determination of Hydrogen Peroxide and Glucose

Synthesis of a Bifunctional Fluorescent Polymer for Cell Imaging and Enzyme Detection

Separation of Time‐Resolved Phenomena in Surface‐Enhanced Raman Scattering of the Photocatalytic Reduction of p‐Nitrothiophenol

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