Assembly of an improved hybrid cascade system for complete ethylene glycol oxidation: Enhanced catalytic performance for an enzymatic biofuel cell

Franco, Jefferson Honorio; Bonaldo, João Victor; Minteer, Shelley D.; Andrade, Adalgisa R. de

doi:10.1016/j.bios.2022.114649

Cited by 4 publications

(2 citation statements)

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“…Bioelectrodes that harness the combined capability of many oxidoreductase enzymes at the same time to catalyze multistep reactions are important for biosensing applications [ 1 , 2 ] and bioconversion of energy [ 3 , 4 ]. Systems that integrate multiple enzymes have been developed for biochemical analysis and for optimizing the profitability of substrate consumption [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, artificial multienzyme systems have been used to construct bioelectrodes that include an integrated one-pot catalytic system [ 1 , 2 , 9 , 10 ]. In addition to biosensing applications, enzyme cascades enable the full oxidation of fuels, which is essential for effective biofuel cells [ 3 , 11 ]. However, the optimization of this type of electro-enzymatic system is difficult and time-consuming to set up.…”

Section: Introductionmentioning

confidence: 99%

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A Conductive Microcavity Created by Assembly of Carbon Nanotube Buckypapers for Developing Electrochemically Wired Enzyme Cascades

Jeerapan,

Nedellec,

Cosnier

2024

Nanomaterials

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We describe the creation of a conductive microcavity based on the assembly of two pieces of carbon nanotube buckypaper for the entrapment of two enzymes, horseradish peroxidase (HRP) and glucose oxidase (GOx), as well as a redox mediator: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS). The hollow electrode, employing GOx, HRP, and the mediator, as an electrochemical enzyme cascade model, is utilized for glucose sensing at a potential of 50 mV vs. Ag/AgCl. This bienzyme electrode demonstrates the ability to oxidize glucose by GOx and subsequently convert H2O2 to water via the electrical wiring of HRP by ABTS. Different redox mediators (ABTS, potassium hexacyanoferrate (III), and hydroquinone) are tested for HRP wiring, with ABTS being the best candidate for the electroenzymatic reduction of H2O2. To demonstrate the possibility to optimize the enzyme cascade configuration, the enzyme ratio is studied with 1 mg HRP combined with variable amounts of GOx (1–4 mg) and 2 mg GOx combined with variable amounts of HRP (0.5–2 mg). The bienzyme electrode shows continuous operational stability for over a week and an excellent storage stability in phosphate buffer, with a decay of catalytic current by only 29% for 1 mM glucose after 100 days.

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