Assembly and operation of an open-source, computer numerical controlled (CNC) robot for performing cranial microsurgical procedures

Rynes, Mathew L.; Ghanbari, Leila; Schulman, Daniel Sousa; Linn, Samantha; Laroque, Michael; Dominguez, Judith; Navabi, Zahra S.; Sherman, Peter G.; Kodandaramaiah, Suhasa B.

doi:10.1038/s41596-020-0318-4

Cited by 21 publications

(13 citation statements)

References 43 publications

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“…The scalp was shaved and sterilized, followed by application of sterile eye ointment (Puralube, Dechra Veterinary Products) to the eyes. Mice were then transferred and affixed to either a standard rodent stereotax (Model 900LS, Kopf Inc.) or an automated robotic surgery platform, the Craniobot 43 , 44 . The scalp above the dorsal cortex was excised using surgical scissors and fascia was removed using a 0.5 mm micro-curette (10080-05, Fine Science Tools).…”

Section: Methodsmentioning

confidence: 99%

Miniaturized head-mounted microscope for whole-cortex mesoscale imaging in freely behaving mice

et al. 2021

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The advent of genetically encoded calcium indicators, along with surgical preparations such as thinned skulls or refractive index matched skulls, have enabled mesoscale cortical activity imaging in head-fixed mice. However, neural activity during unrestrained behavior substantially differs from neural activity in head-fixed animals. For whole-cortex imaging in freely behaving mice, we here present the “mini-mScope,” a wide-field, miniaturized, and head-mounted fluorescence microscope compatible with transparent polymer skull preparations. With a field of view of 8 mm x 10 mm and weighing less than 4 g, the mini-mScope can image most of the mouse dorsal cortex with resolution ranging from 39 to 56 μm. We have used the mini-mScope to record mesoscale calcium activity across the dorsal cortex during sensory-evoked stimuli, open field behaviors, social interactions, and transitions from wakefulness to sleep.

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Section: Methodsmentioning

confidence: 99%

Miniaturized head-mounted microscope for whole-cortex mesoscale imaging in freely behaving mice

et al. 2021

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“…After completion of the electrode patterning, the functionalized PET film was bonded to the interface PCB using anisotropic conductive film (ACF). The functionalized PET film was then epoxy bonded to the polymethyl methacrylate (PMMA) frame of the polymer skull, as described previously 12,33 , to complete the eSee-Shell (Fig. 1c-d).…”

Section: Results Esee-shell Designmentioning

confidence: 99%

“…Such studies have typically used transparent windows for calcium (Ca 2+ ) imaging which allow high-resolution cellular mapping of activities from microcircuits 9 . The advent of large cranial windows [10][11][12] allows either mesoscale mapping of Ca 2+ activity or random access measurement of cellular activity from smaller fields of view (FOV). As large-scale ongoing activity modulates single cells within microcircuits 13 , it is advantageous to simultaneously monitor both cellular and mesoscale activity.…”

mentioning

confidence: 99%

Polymer skulls with integrated transparent electrode arrays for cortex-wide opto-electrophysiological recordings

Donaldson

Navabi

Carter

et al. 2021

Preprint

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Electrophysiological and optical imaging provide complementary neural sensing capabilities – electrophysiological recordings have the highest temporal resolution, while optical imaging allows recording the activities of genetically defined populations at high spatial resolution. Combining these complementary, yet orthogonal modalities to perform simultaneous large-scale, multimodal sensing of neural activity across multiple brain regions would be very powerful. Here we show that transparent, inkjet-printed electrocorticography (ECoG) electrode arrays can be seamlessly integrated with morphologically conformant transparent polymer skulls for multimodal recordings across the cortex. These ‘eSee-Shells’ were implanted on transgenic mice expressing the Ca2+ indicator GCaMP6f in cortical excitatory cells and provided a robust opto-electrophysiological interface for over 100 days. eSee-Shells enable simultaneous mesoscale Ca2+ imaging and ECoG acquisition under anesthesia as well as in awake animals presented with sensory stimuli. eSee-Shells further show sufficient clarity and transparency to observe single-cell Ca2+ signals directly below the electrodes and interconnects. Simultaneous multimodal measurement of cortical dynamics reveals changes in both ECoG and Ca2+ signals that depend on the behavioral state.

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“…The autonomous degree represents the steps that have been automated within the patch-clamping pipeline according to the following: [8] Sealing and break-through. However, it should be noted that these are not the only steps being automated, as the first strides towards the automation of craniotomies have been taken (Ghanbari et al, 2019;Rynes et al, 2020 9% in C57BL/6 mice…”

Section: Procedures For Robotic "Blind" and Two-photon Guided Whole-cell Recordings In Vivomentioning

confidence: 99%

Robotic automation of “blind” and two-photon targeted patch-clamp electrophysiology in vivo

Gonzalez¹,

Kgwarae²,

Annecchino³

et al. 2021

Preprint

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Assembly and operation of an open-source, computer numerical controlled (CNC) robot for performing cranial microsurgical procedures

Cited by 21 publications

References 43 publications

Miniaturized head-mounted microscope for whole-cortex mesoscale imaging in freely behaving mice

Miniaturized head-mounted microscope for whole-cortex mesoscale imaging in freely behaving mice

Polymer skulls with integrated transparent electrode arrays for cortex-wide opto-electrophysiological recordings

Robotic automation of “blind” and two-photon targeted patch-clamp electrophysiology in vivo

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