Assembly and Initial Characterization of a Panel of 85 Genomically Validated Cell Lines from Diverse Head and Neck Tumor Sites

Abstract

Purpose Human cell lines are useful for studying cancer biology and pre-clinically modeling cancer therapy, but can be misidentified and cross contamination is unfortunately common. The purpose of this study was to develop a panel of validated head and neck cell lines representing the spectrum of tissue sites and histologies that could be used for studying the molecular, genetic, and phenotypic diversity of head and neck cancer. Methods A panel of 122 clinically and phenotypically diverse head and neck cell lines from head and neck squamous cell carcinoma (HNSCC), thyroid cancer, cutaneous squamous cell carcinoma, adenoid cystic carcinoma, oral leukoplakia, immortalized primary keratinocytes, and normal epithelium, was assembled from the collections of several individuals and institutions. Authenticity was verified by performing short tandem repeat (STR) analysis. Human papillomavirus (HPV) status and cell morphology were also determined. Results Eighty-five of the 122 cell lines had unique genetic profiles. HPV-16 DNA was detected in 2 cell lines. These 85 cell lines included cell lines from the major head and neck primary tumor sites, and close examination demonstrates a wide range of in vitro phenotypes. Conclusion This panel of 85 genomically validated head and neck cell lines represents a valuable resource for the head and neck cancer research community that can help advance understanding of the disease by providing a standard reference for cell lines that can be utilized for biological as well as preclinical studies.

Neutrophils Increase Oral Squamous Cell Carcinoma Invasion through an Invadopodia-Dependent Pathway

“…The UMSCC47 cell line is derived from a 53-year-old man with a metastatic lateral tongue squamous cell carcinoma (T3N1M0). UMSCC47 has a wild-type TP53 gene and is positive for HPV16 (20). UMSCC1 is derived from a man with a nonmetastatic floor of mouth squamous cell carcinoma (21).…”

“…UMSCC1 is derived from a man with a nonmetastatic floor of mouth squamous cell carcinoma (21). Both UMSCC1 and UMSCC47 were recently shown to represent a unique genetic background and to be free of contamination (20). All cell cultures and experiments were performed in DMEM supplemented with 10% FBS, 100 nmol/L nonessential amino acids (Sigma), penicillin, and streptomycin (100 mg/mL).…”

Trastuzumab upregulates expression of HLA-ABC and T cell costimulatory molecules through engagement of natural killer cells and stimulation of IFNγ secretion

“…38,39 Western blot analysis Cells were lysed in a lysis buffer containing 50 mM TrisHCl (pH 7.4), 150 mM NaCl, 0.5% Igepal CA-630, 50 mM NaF, 1 mM Na 3 VO 4 , 1 mM EGTA, 1 mM EDTA, 1 mM phenylmethylsulfonyl fluoride and protease inhibitor cocktail (SigmaAldrich) and kept on ice for 15 min. The lysates were cleared by centrifugation, and the supernatants were collected.…”

Cancer stem-like cells and thyroid cancer

“…The KAT-4 ATC cell line also expressed CD133. Subsequent studies demonstrated that the ARO cell line and KAT-4 cell line were not authentic thyroid cell lines because they were contaminated by colon cancer cell line HT-29 (Schweppe et al 2008, Zhao et al 2011. In the medullary thyroid carcinoma cell line TT, CD133C tumor-initiating subpopulation derived from drug-exposed FTTiv cells is significantly more resistant to 5-FU and retains the chemoresistant properties upon FTTiv culture propagation (Kucerova et al 2013).…”