Assembly and Immunological Properties of Newcastle Disease Virus-Like Particles Containing the Respiratory Syncytial Virus F and G Proteins

Abstract

Human respiratory syncytial virus (RSV) is a serious respiratory pathogen in infants and

Respiratory syncytial virus-like nanoparticle vaccination induces long-term protection without pulmonary disease by modulating cytokines and T-cells partially through alveolar macrophages

“…38 Infection of unimmunized naïve mice with RSV usually does not induce severe lung histopathology since mice are not highly permissive to RSV. 6,8,23 In this study, we observed a substantial degree of lung histopathology in naïve mice after RSV infection probably due to the nature of 1-year-old aged mice and high lung viral loads. FI-RSV and FG VLP immune mice as well as live RSV previously infected mice (data not shown) were found to be highly effective in controlling lung viral loads by lowering 500-to 1,000-fold.…”

“…The effective control of lung viral loads in FI-RSV immune mice is consistent with that of previous studies. 6,7,9,19,23 Herein, we addressed long-term vaccine safety concerns of FG VLP vaccines and investigated innate and adaptive cellular immune components that are likely responsible for RSV protection or pulmonary inflammation upon RSV infection.…”

“…[5][6][7][8][9] Compared to FI-RSV, immunization with RSV VLP vaccines induced a higher IgG2a/IgG1 antibody ratio, which refers to Th1 immune responses. 5,6 Nanoparticles of FG VLPs were shown to be in a range of 60-120 nm-size particles and produced in insect cells using the recombinant baculovirus expression system. 5 Alveolar macrophages (AMs) are the first defense line of innate immune cells in the respiratory tract.…”

Virus-like particle vaccines containing F or F and G proteins confer protection against respiratory syncytial virus without pulmonary inflammation in cotton rats

“…Chimeric NDV-RSV VLPs in avian cells by transiently transfecting DNA plasmids expressing multiple NDV proteins and chimeric RSV G plus F proteins were not effective in producing F alone VLPs. 5 In the insect cell expression system, full length RSV F or G proteins were effectively incorporated into VLPs. 10 In cotton rats, this study demonstrated that F VLP was more immunogenic and effective in clearing lung viral titers than G VLP although both F VLP and G VLP did not cause weight loss and AHR lung disease after RSV challenge.…”

“…Utilizing the structural proteins (NP, M) of Newcastle disease virus (NDV), chimeric NDV-RSV VLP vaccines were produced in avian cells, which contain the ectodomains of the RSV F and G proteins in a chimeric fusion to the transmembrane and cytoplasmic tail of the NDV proteins. 5,6 NDV-RSV VLP vaccines were immunogenic, and capable of inducing neutralizing antibodies and long-lived protection without overt vaccine-enhanced disease in mice, 5,7,8 and cotton rats. 9 We have previously developed VLP vaccines containing RSV F (F VLP) or RSV G (G VLP) using influenza matrix M1 protein and the recombinant baculovirus / insect cell expression system.…”