Assembly and features of secondary metabolite biosynthetic gene clusters in Streptomyces ansochromogenes

Zhong, Xin; Tian, Yun; Niu, Guoqing; Tan, Huarong

doi:10.1007/s11427-013-4506-0

Cited by 10 publications

(9 citation statements)

References 28 publications

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“…These cluster numbers are surprisingly high, and although many cannot be assigned to production of a certain metabolite, their abundance suggests that the genomes of these A. pullulans varieties represent rich resources for secondary metabolite biosynthesis. In comparison, antiSMASH predicted 45 T1 polyketide synthase/non-ribosomal peptide synthase/ dmat gene clusters in Penicillium chrysogenum , 29 clusters in Aspergillus fumigatus [66], and a total of 35 putative gene clusters in a draft genome sequence of Streptomyces ansochromogenes (Streptomycetes are known for their complex secondary metabolism) [67]. For example, lantipeptide biosynthetic clusters from A. pullulans var.…”

Section: Resultsmentioning

confidence: 99%

Genome sequencing of four Aureobasidium pullulans varieties: biotechnological potential, stress tolerance, and description of new species

et al. 2014

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BackgroundAureobasidium pullulans is a black-yeast-like fungus used for production of the polysaccharide pullulan and the antimycotic aureobasidin A, and as a biocontrol agent in agriculture. It can cause opportunistic human infections, and it inhabits various extreme environments. To promote the understanding of these traits, we performed de-novo genome sequencing of the four varieties of A. pullulans.ResultsThe 25.43-29.62 Mb genomes of these four varieties of A. pullulans encode between 10266 and 11866 predicted proteins. Their genomes encode most of the enzyme families involved in degradation of plant material and many sugar transporters, and they have genes possibly associated with degradation of plastic and aromatic compounds. Proteins believed to be involved in the synthesis of pullulan and siderophores, but not of aureobasidin A, are predicted. Putative stress-tolerance genes include several aquaporins and aquaglyceroporins, large numbers of alkali-metal cation transporters, genes for the synthesis of compatible solutes and melanin, all of the components of the high-osmolarity glycerol pathway, and bacteriorhodopsin-like proteins. All of these genomes contain a homothallic mating-type locus.ConclusionsThe differences between these four varieties of A. pullulans are large enough to justify their redefinition as separate species: A. pullulans, A. melanogenum, A. subglaciale and A. namibiae. The redundancy observed in several gene families can be linked to the nutritional versatility of these species and their particular stress tolerance. The availability of the genome sequences of the four Aureobasidium species should improve their biotechnological exploitation and promote our understanding of their stress-tolerance mechanisms, diverse lifestyles, and pathogenic potential.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-549) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Genome sequencing of four Aureobasidium pullulans varieties: biotechnological potential, stress tolerance, and description of new species

et al. 2014

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“…To verify the corresponding gene cluster(s) responsible for the newly emerged bioactive compound(s) in ⌬adpA, we isolated the total RNA from both WT strain and ⌬adpA grown in MS, SP, and GP media at different time courses, respectively. Transcription of the core genes (27) in each PKS and NRPS secondary metabolic gene cluster in S. ansochromogenes genome was analyzed by reverse transcription-PCR (RT-PCR). The gene clusters silent in WT strain but activated in ⌬adpA are potentially responsible for the biosynthesis of the new bioactive compound.…”

Section: Activation Of a Cryptic Oviedomycin Biosynthetic Gene Clustementioning

confidence: 99%

“…Total RNA was isolated from S. ansochromogenes and its derivatives, and RT-PCR and qRT-PCR were performed as described previously (27,44).…”

Section: Rna Isolation Rt-pcr and Qrt-pcrmentioning

confidence: 99%

“…Our previous results indicated that AdpA not only influenced morphological differentiation but also significantly affected nikkomycin production by directly controlling sanG, which encodes a pathway-specific transcriptional activator (26). antiSMASH analysis of S. ansochromogenes draft genome sequence indicated that 35 secondary metabolite gene clusters exist in the genome (27), of which only two corresponding secondary metabolites (nikkomycin and tylosin analogues) were identified. Therefore, it is expected to obtain more bioactive metabolites from the cryptic gene clusters by adpA manipulation.…”

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confidence: 99%

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Activation and mechanism of a cryptic oviedomycin gene cluster via the disruption of a global regulatory gene, adpA, in Streptomyces ansochromogenes

Zhang

Zhuo

et al. 2017

Journal of Biological Chemistry

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Genome sequencing analysis has revealed at least 35 clusters of likely biosynthetic genes for secondary metabolites in Disruption of encoding a global regulator (AdpA) resulted in the failure of nikkomycin production, whereas other antibacterial activities against ,, and were observed with the fermentation broth of ΔadpA but not with that of the wild-type strain. Transcriptional analysis showed that a cryptic gene cluster (), which shows high identity with an oviedomycin biosynthetic gene cluster (), was activated in ΔadpA. The corresponding product of was characterized as oviedomycin by MS and NMR spectroscopy. To understand the molecular mechanism of activation, the roles of six regulatory genes situated in the cluster were investigated. Among them, proteins encoded by co-transcribed genes and are positive regulators of AdpA directly represses the transcription of and Co-overexpression of and can relieve the repression of AdpA on transcription and effectively activate oviedomycin biosynthesis. The promoter of is identified as the direct target of OvmZ and OvmW. This is the first report that AdpA can simultaneously activate nikkomycin biosynthesis but repress oviedomycin biosynthesis in one strain. Our findings provide an effective strategy that is able to activate cryptic secondary metabolite gene clusters by genetic manipulation of global regulatory genes.

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“…Xi et al (2012) estimated that 27.5% of the isolates tested that belonged to the genus Streptomyces (34 isolates) showed antimicrobial activity, and it has been shown that the strains where both pks and nrps genes were identified produced a significantly higher number of secondary metabolites. Zhong et al (2013) demonstrated the assembly and features of secondary metabolite biosynthetic gene clusters with pks and nrps in S. ansochromogenes. In the current study, we provided evidence for the presence of both genes (nrps and pks) in the strain S. ansochromogenes TUR-10, suggesting that the production of the secondary metabolites might be performed by two metabolic routes.…”

Section: Discussionmentioning

confidence: 97%

Streptomyces ansochromogenes Tur-10 produces a substance with antifungal bioactivity

Vasconcelos¹,

Fontes²,

Lins³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The increased incidence of fungal infections and the development of drug resistance have led to the search for microorganisms capable of producing bioactive metabolites with antifungal activity. Among these microorganisms, Streptomyces spp are distinguished mainly owing to their potential to secrete bioactive molecules. The aim of this study was to evaluate the production of secondary metabolites by Streptomyces sp TUR-10 against 12 fungal clinical isolates (yeast and filamentous fungi). In the preliminary screening, Streptomyces sp TUR-10 showed activity against 75% of the clinical isolates, and was selected for fermentation. In this assay, we tested three different media (MPE, M1, and ISP-4) for 96 h at pH 7.0 and 30°C for the production of bioactive metabolites. Increased production of bioactive compounds was observed when using the MPE medium for 48 h, with good activity against Candida pelliculosa. The minimum inhibitory concentration showed significant antifungal activity values ranging from 15.6 to 250 µg/mL. The actinobacterium was characterized by 16S rRNA analysis N.M. Vasconcelos et al. 5436©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 5435-5444 (2015) and the pattern suggested that the isolate studied belonged to the species Streptomyces ansochromogenes. The biotechnological potential of this strain was also demonstrated by the detection of the nrps and pks genes. These results indicate the production of secondary metabolites of biotechnological interest by actinobacteria from the rhizosphere, suggesting great potential for further research.

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Assembly and features of secondary metabolite biosynthetic gene clusters in Streptomyces ansochromogenes

Cited by 10 publications

References 28 publications

Genome sequencing of four Aureobasidium pullulans varieties: biotechnological potential, stress tolerance, and description of new species

Genome sequencing of four Aureobasidium pullulans varieties: biotechnological potential, stress tolerance, and description of new species

Activation and mechanism of a cryptic oviedomycin gene cluster via the disruption of a global regulatory gene, adpA, in Streptomyces ansochromogenes

Streptomyces ansochromogenes Tur-10 produces a substance with antifungal bioactivity

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