Assembly and disassembly intermediates of maize streak geminivirus

Bennett, Antonette; Rodríguez, David; Lister, Samantha; Boulton, Margaret I.; McKenna, Robert; Agbandje‐McKenna, Mavis

doi:10.1016/j.virol.2018.09.011

Cited by 9 publications

(4 citation statements)

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“…Mass spectrometry of tryptic digests of the protein in the bands confirmed their identity as the expected geminivirus coat proteins (Figure S2). As in previous studies, [25,[47][48][49], additional faster migrating forms of the coat protein can be seen. The presence of coat protein in fractions from the middle of the gradient suggests that, unlike the encapsidation of alphasatellite DNA, all the coat proteins were able to form particles with full-length cssDNA.…”

Section: Transencapsidation Of Ayvv Genome-length Dnasupporting

confidence: 83%

Requirements for the Packaging of Geminivirus Circular Single-Stranded DNA: Effect of DNA Length and Coat Protein Sequence

Saunders

Richardson

Lawson

et al. 2020

Viruses

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Geminivirus particles, consisting of a pair of twinned isometric structures, have one of the most distinctive capsids in the virological world. Until recently, there was little information as to how these structures are generated. To address this, we developed a system to produce capsid structures following the delivery of geminivirus coat protein and replicating circular single-stranded DNA (cssDNA) by the infiltration of gene constructs into plant leaves. The transencapsidation of cssDNA of the Begomovirus genus by coat protein of different geminivirus genera was shown to occur with full-length but not half-length molecules. Double capsid structures, distinct from geminate capsid structures, were also generated in this expression system. By increasing the length of the encapsidated cssDNA, triple geminate capsid structures, consisting of straight, bent and condensed forms were generated. The straight geminate triple structures generated were similar in morphology to those recorded for a potato-infecting virus from Peru. These finding demonstrate that the length of encapsidated DNA controls both the size and stability of geminivirus particles.

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Section: Transencapsidation Of Ayvv Genome-length Dnasupporting

confidence: 83%

Requirements for the Packaging of Geminivirus Circular Single-Stranded DNA: Effect of DNA Length and Coat Protein Sequence

Saunders

Richardson

Lawson

et al. 2020

Viruses

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“…Studies on a monopartite begomovirus, tomato yellow leaf curl virus (TYLCV), have investigated this aspect in closer detail (Gorovits et al, 2013a(Gorovits et al, , 2013b. Recently, a double band of the maize streak virus CP has been assigned to a pentameric assembly of the CP in contrast to a single band, the lower one of the double band, found in single, icosahedral capsids and geminate particles (Bennett et al, 2018). It has been speculated that the shift is due to a post-translational modification of the CP (Bennett et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Different forms of African cassava mosaic virus capsid protein within plants and virions

et al. 2019

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“…While the capsids of tombusviruses have been shown to adopt a T=1 icosahedral conformation, rather than the usual T=3, when the R-domain is partially or totally removed ( 61 ), we have not seen a correlation between the length of capsid protein domains and genome size in our data set that could be indicative of alternative capsid arrangements. Furthermore, no packaging dynamics relating genome size and virion T-number arrangement have been determined in CRESS-DNA viruses, although subgenomic elements of geminiviruses can be packaged in nongeminate capsids ( 63 , 64 ).…”

Section: Resultsmentioning

confidence: 99%

Unveiling Crucivirus Diversity by Mining Metagenomic Data

Higuera

Kasun

Torrance

et al. 2020

mBio

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The discovery of cruciviruses revealed the most explicit example of a common protein homologue between DNA and RNA viruses to date. Cruciviruses are a novel group of circular Rep-encoding single-stranded DNA (ssDNA) (CRESS-DNA) viruses that encode capsid proteins that are most closely related to those encoded by RNA viruses in the family Tombusviridae. The apparent chimeric nature of the two core proteins encoded by crucivirus genomes suggests horizontal gene transfer of capsid genes between DNA and RNA viruses. Here, we identified and characterized 451 new crucivirus genomes and 10 capsid-encoding circular genetic elements through de novo assembly and mining of metagenomic data. These genomes are highly diverse, as demonstrated by sequence comparisons and phylogenetic analysis of subsets of the protein sequences they encode. Most of the variation is reflected in the replication-associated protein (Rep) sequences, and much of the sequence diversity appears to be due to recombination. Our results suggest that recombination tends to occur more frequently among groups of cruciviruses with relatively similar capsid proteins and that the exchange of Rep protein domains between cruciviruses is rarer than intergenic recombination. Additionally, we suggest members of the stramenopiles/alveolates/Rhizaria supergroup as possible crucivirus hosts. Altogether, we provide a comprehensive and descriptive characterization of cruciviruses. IMPORTANCE Viruses are the most abundant biological entities on Earth. In addition to their impact on animal and plant health, viruses have important roles in ecosystem dynamics as well as in the evolution of the biosphere. Circular Rep-encoding single-stranded (CRESS) DNA viruses are ubiquitous in nature, many are agriculturally important, and they appear to have multiple origins from prokaryotic plasmids. A subset of CRESS-DNA viruses, the cruciviruses, have homologues of capsid proteins encoded by RNA viruses. The genetic structure of cruciviruses attests to the transfer of capsid genes between disparate groups of viruses. However, the evolutionary history of cruciviruses is still unclear. By collecting and analyzing cruciviral sequence data, we provide a deeper insight into the evolutionary intricacies of cruciviruses. Our results reveal an unexpected diversity of this virus group, with frequent recombination as an important determinant of variability.

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Assembly and disassembly intermediates of maize streak geminivirus

Cited by 9 publications

References 50 publications

Requirements for the Packaging of Geminivirus Circular Single-Stranded DNA: Effect of DNA Length and Coat Protein Sequence

Requirements for the Packaging of Geminivirus Circular Single-Stranded DNA: Effect of DNA Length and Coat Protein Sequence

Different forms of African cassava mosaic virus capsid protein within plants and virions

Unveiling Crucivirus Diversity by Mining Metagenomic Data

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