Assembly and comparative analysis of the complete mitochondrial genome of Bupleurum chinense DC

Abstract

Background Bupleurum chinense(B. chinense) is a plant that is widely distributed globally and has strong pharmacological effects. Though the chloroplast(cp) genome of B. chinense has been studied, no reports regarding the mitochondrial(mt) genome of B. chinense have been published yet. Results The mt genome of B.chinense was assembled and functionally annotated. The circular mt genome of B. chinense was 435,023 bp in length, and 78 genes, including 39 protein-coding genes, 35 tRNA genes, and 4 rRNA genes, were annotated. Repeat sequences were analyzed and sites at which RNA editing would occur were predicted. Gene migration was observed to occur between the mt and cp genomes of B. chinense via the detection of homologous gene fragments. In addition, the sizes of plant mt genomes and their GC content were analyzed and compared. The sizes of mt genomes of plants varied greatly, but their GC content was conserved to a greater extent during evolution. Ka/Ks analysis was based on code substitutions, and the results showed that most of the coding genes were negatively selected. This indicates that mt genes were conserved during evolution. Conclusion In this study, we assembled and annotated the mt genome of the medicinal plant B. chinense. Our findings provide extensive information regarding the mt genome of B. chinense, and help lay the foundation for future studies on the genetic variations, phylogeny, and breeding of B. chinense via an analysis of the mt genome.

Complete mitochondrial genome assembly and comparison of Camellia sinensis var. Assamica cv. Duntsa

“…This investigation uncovered 536 RNA editing sites within 41 genes.After RNA editing, the waterrepelling properties of 43.74 percent of the amino acids did not change, 7.29% became hydrophilic, and 48.22% became hydrophobic. There are consistent results in the mitochondrial genome of B. chinense (Qiao et al, 2022).…”

“…The circular C.duntsa mitochondrial genome that was published here is 1,081,966 bp long, and it has a GC content of 45.62%. The GC content was comparable to that of other sequenced plant mitochondrial genomes, for example, B. chinense, 45.68% (Qiao et al, 2022);B. juncea, 45.24% (Chang et al, 2011).…”

Complete mitochondrial genome assembly and comparative analysis of Colocasia esculenta

“…The GC contents of circular molecules 1-5 were 46.65%, 46.06%, 46.17%, 45.32%, and 46.26%, respectively. These mitogenomes are similar to those of many other plant mitogenomes, such as those of Bupleurum chinense [40] (45.68%), Brassica juncea [41](45.24%),…”

Assembly and comparative analysis of the complete mitochondrial genome of Trigonella foenum-graecum L.

“…Dispersed repetitive sequences are repetitive units that are present in a scattered form throughout the genome [17]. A total of 202 dispersed repeat sequences were detected in the T. foenum-graecum mt genome, including 108 forward repeats (F) and 94 palindrome repeats (P) of two repeat types, with repeat lengths mostly concentrated between 30-60 (83).…”