Assembled denitrifying consortia for efficient nitrate removal under low-COD/N conditions

Jiang, Meng; Wu, Yougui; He, Penghui; Hu, Shiyin; Li, Qingxi; Chen, Shouwen

doi:10.1016/j.cej.2023.141655

Cited by 6 publications

(4 citation statements)

References 44 publications

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“…Thauera was the most common genus in denitrification systems as it could utilize both organic matter as a carbon source for heterotrophic denitrification and H 2 as an electron donor for hydrogen autotrophic denitrification [45]. Dokdonella and Acinetobacter were also the two more common genera with denitrification capability [46,47]. Some researchers isolated Acinetobacter from a hydrogenotrophic reactor and demonstrated that it played an important role in the denitrification process of hydrogenotrophic denitrification [48].…”

Section: Discussionmentioning

confidence: 99%

Enhanced Nitrate Nitrogen Removal from Constructed Wetland via Fe3O4/Granular Activated Carbon Anode Microbial Electrolysis Cell under Low C/N Ratio

Yang,

Tan,

Huang

et al. 2024

Water

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In this study, a constructed wetland–Fe3O4/granular activated carbon anode microbial electrolysis cell (CW-FMEC) was constructed to enhance denitrification in low COD/N ratio wastewater. The introduction of Fe3O4 boosted the expression of functional genes involved in the denitrification pathway, and the abundance of narG, nirS, and nosZ increased by 99.29%, 70.54%, and 132.18%, respectively, compared to CW. In addition, the content of c-type cytochromes (c-Cyts) and EPS were also enhanced in the CW-FMEC. The microbial communities study displayed that Thauera, Dechloromonas, and Arenimonas became the main genera for denitrification. The denitrification performance at different COD/N ratios was investigated in depth. Under optimal working circumstances, the CW-FMEC had an excellent nitrate removal rate (88.9% ± 1.12%) while accumulating nearly no NO2−-N or NH4+-N in the effluent. This study provides a new direction for the development of CW-MEC and accelerates its implementation.

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Section: Discussionmentioning

confidence: 99%

Enhanced Nitrate Nitrogen Removal from Constructed Wetland via Fe3O4/Granular Activated Carbon Anode Microbial Electrolysis Cell under Low C/N Ratio

Yang,

Tan,

Huang

et al. 2024

Water

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“…and Comamonas nitrativorans achieved a 98.4% total nitrogen removal rate at a COD/N ratio of 3.2. In addition, some studies result showed that Pseudomonas stutzeri was critically involved in NOx reduction [25]. Paracetamol is one of the most used pharmaceuticals in environments, such as surface and ground waters, sediments, soils or even plants, considering it is introduced mainly from the discharge of wastewater.…”

Section: Discussionmentioning

confidence: 99%

The Mass Spectrometry Identification, Antimicrobial Genes Detection, and Proteomics Analysis of Stutzerimonas stutzeri Strain Was Isolated from Industrial Wastewater

Wang,

Sun,

Chen

et al. 2023

Separations

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A large amount of organic matter, heavy metals, and even antibiotics are present in industrial wastewater, aquaculture waters, and various types of sewage, along with abundant microorganisms. To date, only a few studies involving the resistance and proteomics of Stutzerimonas stutzeri in high-salt wastewater are available. Herein, a comprehensive assessment of a newly isolated Stutzerimonas stutzeri strain, which is present in high-salt wastewater, was performed using mass spectrometry, genetic identification, and biochemical analysis to characterize the genetic and biochemical properties. Growth experiments revealed that the Stutzerimonas stutzeri strain had a moderate growth rate in nutrient broth, and the bacterial count was not high. Further analysis highlighted an apparent susceptibility of this strain to most antibiotics but some resistance to chloramphenicol and minocycline. A resistance gene assay results showed that the gene gyrB was associated with antibiotic resistance in this Stutzerimonas stutzeri strain. Proteomic analysis revealed for the first time the co-existence of two drug-resistance-related proteins (Multidrug/solvent RND membrane fusion protein and MexE) in Stutzerimonas stutzeri. Moreover, Stutzerimonas stutzeri isolated from high-salt wastewater was subjected to drug resistance gene detection, and the total protein of Stutzerimonas stutzeri was detected by protein mass spectrometry analysis. The subcellular classification shows that the 50 proteins with the highest abundance are divided into cell inner membrane, cell outer membrane, cytoplasm, cytoplasmic side, membrane, multi-pass membrane protein, and peripheral membrane protein, among which the proportion of cytoplasmic components is the highest. Overall, this study’s findings provide a new perspective for further research on the characteristics of Stutzerimonas stutzeri in high-salt wastewater.

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“…In recent biotechnological applications of bacteria for the remediation of NO 3 −contaminated water, the focus remains primarily on known and benchmarked bacterial isolates used as consortia. In studies conducted by Jiang et al (2023), an enriched consortium from a sewage treatment plant contained model denitrifiers: P. stutzeri and Paracoccus sp., which were reported to have participated in the removal of NO 3 − , NO 2 − , N 2 O, and total nitrogen (TN). Batch bioreactors are common in experimental bioremediation studies, and sewage as a source of wide microbial communities has become a source of various other denitrifying bacteria (Wang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Batch bioreactors are common in experimental bioremediation studies, and sewage as a source of wide microbial communities has become a source of various other denitrifying bacteria (Wang et al, 2022). In most denitrification studies in which enriched consortia are used, dynamics are monitored with the removal of dissolved nitrogen species: NO 3 − , NO 2 − , NH 4 + , and TN, without validation if N 2 O (potent greenhouse gas) has also been reduced to N 2 , which is an environmentally friendly gas and desired by-product to be emitted by a denitrification system (Adouani et al, 2015;Wang et al, 2022;Jiang et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Metagenomic assessment of nitrate-contaminated mine wastewaters and optimization of complete denitrification by indigenous enriched bacteria

Moloantoa

Khetsha

Kana

et al. 2023

Front. Environ. Sci.

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Nitrate contamination in water remains to be on the rise globally due to continuous anthropogenic activities, such as mining and farming, which utilize high amounts of ammonium nitrate explosives and chemical-NPK-fertilizers, respectively. This study presents insights into the development of a bioremediation strategy to remove nitrates (NO3−) using consortia enriched from wastewater collected from a diamond mine in Lesotho and a platinum mine in South Africa. A biogeochemical analysis was conducted on the water samples which aided in comparing and elucidating their unique physicochemical parameters. The chemical analysis uncovered that both wastewater samples contained over 120 mg/L of NO3− and over 250 mg/L of sulfates (SO42-), which were both beyond the acceptable limit of the environmental surface water standards of South Africa. The samples were atypical of mine wastewaters as they had low concentrations of dissolved heavy metals and a pH of over 5. A metagenomic analysis applied to study microbial diversities revealed that both samples were dominated by the phyla Proteobacteria and Bacteroidetes, which accounted for over 40% and 15%, respectively. Three consortia were enriched to target denitrifying bacteria using selective media and then subjected to complete denitrification experiments. Denitrification dynamics and denitrifying capacities of the consortia were determined by monitoring dissolved and gaseous nitrogen species over time. Denitrification optimization was carried out by changing environmental conditions, including supplementing the cultures with metal enzyme co-factors (iron and copper) that were observed to promote different stages of denitrification. Copper supplemented at 50 mg/L was observed to be promoting complete denitrification of over 500 mg/L of NO3−, evidenced by the emission of nitrogen gas (N2) that was more than nitrous oxide gas (N2O) emitted as the terminal by-product. Modification and manipulation of growth conditions based on the microbial diversity enriched proved that it is possible to optimize a bioremediation system that can reduce high concentrations of NO3−, while emitting an environmentally-friendly N2 instead of N2O, that is, a greenhouse gas. Data collected and discussed in this research study can be used to model an upscale NO3− bioremediation system aimed to remove nitrogenous and other contaminants without secondary contamination.

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Assembled denitrifying consortia for efficient nitrate removal under low-COD/N conditions

Cited by 6 publications

References 44 publications

Enhanced Nitrate Nitrogen Removal from Constructed Wetland via Fe3O4/Granular Activated Carbon Anode Microbial Electrolysis Cell under Low C/N Ratio

Enhanced Nitrate Nitrogen Removal from Constructed Wetland via Fe3O4/Granular Activated Carbon Anode Microbial Electrolysis Cell under Low C/N Ratio

The Mass Spectrometry Identification, Antimicrobial Genes Detection, and Proteomics Analysis of Stutzerimonas stutzeri Strain Was Isolated from Industrial Wastewater

Metagenomic assessment of nitrate-contaminated mine wastewaters and optimization of complete denitrification by indigenous enriched bacteria

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