Assays to Assess Virulence of Xanthomonas axonopodis pv. manihotis on Cassava

Cohn, Megan; Shybut, Mikel; Dahlbeck, Douglas; Staskawicz, Brian J.

doi:10.21769/bioprotoc.1522

Cited by 4 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacterial virulence is usually assessed on infiltration of leaves of adult 2‐ to 4‐month‐old plants through bacterial growth analysis, bacterial movement through the leaf, and symptom development (compilation of protocols in Cohn et al, 2015 ). Bacterial growth and movement within the host quantitatively reflect the ability to grow locally and to migrate through the xylem, while symptom development is a qualitative estimation of the Xpm capacity to induce water‐soaking lesions, which might improve bacterial fitness.…”

Section: Disease Phenotyping Tools Under Controlled Conditionsmentioning

confidence: 99%

Cassava diseases caused byXanthomonas phaseolipv.manihotisandXanthomonas cassavae

Zárate-Chaves¹,

Cruz

López

et al. 2021

Molecular Plant Pathology

View full text Add to dashboard Cite

Cassava is an important staple crop as a source of food and income for hundreds of millions of people in tropical countries. This major crop is threatened by several pests and pathogens that significantly affect productivity. Among bacterial pathogens, the vascular and systemic Xanthomonas phaseoli pv. manihotis (Xpm) has received considerable attention due to its devastating potential in the tropics and scientific importance worldwide (Mansfield et al., 2012). This pathogen profile describes the main hallmarks of this pathosystem and updates several reviews and original articles on the subject.Although more bacterial pathogens infect cassava, information in the literature is scarce. In this regard, we also present a compilation of information for a second xanthomonad infecting cassava, the nonvascular pathogen X. cassavae. | D IS E A S E DE SCRIP TI ON AND EPIDEMI OLOGY | Cassava bacterial blight symptomsCassava bacterial blight (CBB), which is caused by the bacterial pathogen Xpm, is characterized by a range of symptoms that

show abstract

Section: Disease Phenotyping Tools Under Controlled Conditionsmentioning

confidence: 99%

Cassava diseases caused byXanthomonas phaseolipv.manihotisandXanthomonas cassavae

Zárate-Chaves¹,

Cruz

López

et al. 2021

Molecular Plant Pathology

View full text Add to dashboard Cite

show abstract

“…Serial dilutions were plated on the appropriate selection plus CHX to inhibit fungal growth. A detailed description of the midvein growth assay protocol is given in Cohn et al (2015).…”

Section: Plant Inoculations and Virulence Assaysmentioning

confidence: 99%

Comparison of gene activation by two TAL effectors from Xanthomonas axonopodis pv. manihotis reveals candidate host susceptibility genes in cassava

Cohn

Morbitzer

Lahaye

et al. 2016

Molecular Plant Pathology

Self Cite

View full text Add to dashboard Cite

Xanthomonas axonopodis pv. manihotis (Xam) employs transcription activator-like (TAL) effectors to promote bacterial growth and symptom formation during infection of cassava. TAL effectors are secreted via the bacterial type III secretion system into plant cells, where they are directed to the nucleus, bind DNA in plant promoters and activate the expression of downstream genes. The DNA-binding activity of TAL effectors is carried out by a central domain which contains a series of repeat variable diresidues (RVDs) that dictate the sequence of bound nucleotides. TAL14Xam668 promotes virulence in Xam strain Xam668 and has been shown to activate multiple cassava genes. In this study, we used RNA sequencing to identify the full target repertoire of TAL14Xam668 in cassava, which includes over 50 genes. A subset of highly up-regulated genes was tested for activation by TAL14CIO151 from Xam strain CIO151. Although TAL14CIO151 and TAL14Xam668 differ by only a single RVD, they display differential activation of gene targets. TAL14CIO151 complements the TAL14Xam668 mutant defect, implying that shared target genes are important for TAL14Xam668 -mediated disease susceptibility. Complementation with closely related TAL effectors is a novel approach to the narrowing down of biologically relevant susceptibility genes of TAL effectors with multiple targets. This study provides an example of how TAL effector target activation by two strains within a single species of Xanthomonas can be dramatically affected by a small change in RVD-nucleotide affinity at a single site, and reflects the parameters of RVD-nucleotide interaction determined using designer TAL effectors in transient systems.

show abstract

Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes

Hulten

Chatterjee

Burg

2019

Methods in Molecular Biology

View full text Add to dashboard Cite

Assays to Assess Virulence of Xanthomonas axonopodis pv. manihotis on Cassava

Cited by 4 publications

References 5 publications

Cassava diseases caused byXanthomonas phaseolipv.manihotisandXanthomonas cassavae

Cassava diseases caused byXanthomonas phaseolipv.manihotisandXanthomonas cassavae

Comparison of gene activation by two TAL effectors from Xanthomonas axonopodis pv. manihotis reveals candidate host susceptibility genes in cassava

Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes

Contact Info

Product

Resources

About