Assays of Gamma‐Glutamyl Transferase and Carbohydrate‐Deficient Transferrin Combination from Maternal Serum Improve the Detection of Prenatal Alcohol Exposure

Niemelä, Onni; Niemelä, Solja; Ritvanen, Annukka; Gissler, Mika; Bloigu, Aini; Vääräsmäki, Marja; Kajantie, Eero; Werler, Martha M.; Surcel, Heljä‐Marja

doi:10.1111/acer.13207

Cited by 16 publications

(18 citation statements)

References 51 publications

(62 reference statements)

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“…Genotype data were in Hardy-Weinberg equilibrium. Serum carbohydrate-deficient transferrin (CDT) and GGT-CDT combination were determined as biomarkers of alcohol consumption using previously established protocols (31).…”

Section: Study Designsmentioning

confidence: 99%

Hydroxysteroid 17-β dehydrogenase 13 variant increases phospholipids and protects against fibrosis in nonalcoholic fatty liver disease

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Section: Study Designsmentioning

confidence: 99%

Hydroxysteroid 17-β dehydrogenase 13 variant increases phospholipids and protects against fibrosis in nonalcoholic fatty liver disease

et al. 2020

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“…We used the following formula: 0.8 x ln(GGT) + 1.3 x ln(%CDT) to calculate GGT-CDT. (25,26,32) Fibrinogen was determined using the adapted Clauss method (Multi brin U-test, BCS coagulation analyser, Dade Behring, Deer eld, IL, USA). Fibrinogen γ' was quanti ed with an ELISA using a 2.G2.H9 mouse monoclonal coating antibody against the human γ' sequence (Santa Cruz Biotechnology, Santa Cruz, USA) for antigen capture and a goat polyclonal antibody against human brinogen (Antibody 7539, Abcam for development, Cambridge, USA).…”

Section: Biochemical Analysesmentioning

confidence: 99%

“…(23) By mathematically combining these biomarkers, GGT-CDT, the diagnostic accuracy exceeds those of its parent components in detection of alcohol consumption. (24)(25)(26) To clarify the interrelationships of these factors, we conducted a study with the aim to establish, rst, what associations there might be between total and γ' brinogen concentration or brin plasma clot properties and alcohol intake/markers; and second, to determine whether there are interactions between these alcohol biomarkers and fourteen brinogen and two FXIII gene SNPs, that we identi ed from the literature to be of importance, in relation to total and γ' brinogen concentrations and clot characteristics.…”

Section: Introductionmentioning

confidence: 99%

The association of alcohol with circulating total fibrinogen and plasma clot density is mediated by fibrinogen and FXIII genotypes

Rautenbach

Nienaber-Rousseau

Pieters

2020

Preprint

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Background Alcohol consumption is associated with haemostasis and so may influence cardiovascular conditions. It is unknown whether the association of alcohol with total and γ’ fibrinogen concentrations, as well as clot structure, are modulated by fibrinogen and factor (F)XIII single nucleotide polymorphisms (SNPs). Methods Total fibrinogen, γ’ fibrinogen and clot properties of 2010 healthy black South Africans were measured in relation to alcohol intake as well as its markers – gamma-glutamyltransferase (GGT), percentage carbohydrate deficient transferrin (%CDT), aspartate aminotransferase (AST), and alanine aminotransferase (ALT). Fourteen fibrinogen and two SNPs in the FXIII gene were genotyped to determine their influence. Results Alcohol intake and its markers correlated negatively with fibrinogen and clot lysis time (CLT) as well as with most of the clot properties. Percentage γ’ fibrinogen correlated positively with AST and negatively with alcohol intake. We then stratified for alcohol intake and found inverse associations between γ’ fibrinogen and both %CDT and GGT–CDT in consumers, but the positive association with AST remained only in abstainers. Alcohol intake and its markers modulated the influence of fibrinogen SNPs on total fibrinogen concentrations and the fibrinogen SNPs as well as an FXIII SNP on clot density (all p <0.004). Conclusion/s We show for the first time that some individuals harbour certain genotypes that, in combination with alcohol consumption, might predispose or protect them from haemostatic factors that might lead to the development of cardiovascular disease. Studies are needed to clarify the mechanisms related to the interplay between alcohol and gene variants observed here.

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“…We used the following formula: 0.8 x ln(GGT)+1.3 x ln(%CDT) to calculate GGT-CDT. (25,26,32) Fibrinogen was determined using the adapted Clauss method (Multi brin U-test, BCS coagulation analyser, Dade Behring, Deer eld, IL, USA). Fibrinogen g' was quanti ed with an ELISA using a 2.G2.H9 mouse monoclonal coating antibody against the human g' sequence (Santa Cruz Biotechnology, Santa Cruz, USA) for antigen capture and a goat polyclonal antibody against human brinogen (Antibody 7539, Abcam for development, Cambridge, USA).…”

Section: Biochemical Analysesmentioning

confidence: 99%

“…(23) By mathematically combining these biomarkers, GGT-CDT, the diagnostic accuracy exceeds those of its parent components in detection of alcohol consumption. (24)(25)(26) To clarify the interrelationships of these factors, we conducted a study with the aim to establish, rst, what associations there might be between total and g' brinogen concentration or brin plasma clot properties and alcohol intake/markers; and second, to determine whether there are interactions between these alcohol biomarkers and fourteen brinogen and two FXIII gene SNPs, that we identi ed from the literature to be of importance, in relation to total and g' brinogen concentrations and clot characteristics.…”

Section: Introductionmentioning

confidence: 99%

The association of alcohol with circulating total fibrinogen and plasma clot density is mediated by fibrinogen and FXIII genotypes

Rautenbach

Nienaber-Rousseau

Pieters

2020

Preprint

View full text Add to dashboard Cite

Background: Alcohol consumption is associated with haemostasis and so may influence cardiovascular conditions. It is unknown whether the association of alcohol with total and g’ fibrinogen concentrations, as well as clot structure, are modulated by fibrinogen and factor (F)XIII single nucleotide polymorphisms (SNPs).Methods: Total fibrinogen, g’ fibrinogen and clot properties of 2010 healthy Africans residing in South Africa were measured in relation to alcohol intake as well as its markers – gamma-glutamyltransferase (GGT), percentage carbohydrate deficient transferrin (%CDT), aspartate aminotransferase (AST), and alanine aminotransferase (ALT). Fourteen fibrinogen and two SNPs in the FXIII gene were genotyped to determine their influence.Results: Alcohol intake and its markers correlated negatively with fibrinogen and clot lysis time (CLT) as well as with most of the clot properties. Percentage g’ fibrinogen correlated positively with AST and negatively with alcohol intake. We then stratified for alcohol intake and found inverse associations between g’ fibrinogen and both %CDT and GGT–CDT in consumers, but the positive association with AST remained only in abstainers. Alcohol intake and its markers modulated the influence of fibrinogen SNPs on total fibrinogen concentrations and the fibrinogen SNPs as well as an FXIII SNP on clot density (all p <0.004).Conclusion/s: We show for the first time that some individuals harbour certain genotypes that, in combination with alcohol consumption, might predispose or protect them from haemostatic factors that might lead to the development of cardiovascular disease. Studies are needed to clarify the mechanisms related to the interplay between alcohol and gene variants observed here.

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Assays of Gamma‐Glutamyl Transferase and Carbohydrate‐Deficient Transferrin Combination from Maternal Serum Improve the Detection of Prenatal Alcohol Exposure

Abstract: Combination of GGT and CDT improves the identification of prenatal alcohol exposure and associated high-risk pregnancies. A more systematic use of biomarkers may help intervention efforts to prevent alcohol-induced adverse effects on fetal development.

Cited by 16 publications

References 51 publications

Hydroxysteroid 17-β dehydrogenase 13 variant increases phospholipids and protects against fibrosis in nonalcoholic fatty liver disease

Hydroxysteroid 17-β dehydrogenase 13 variant increases phospholipids and protects against fibrosis in nonalcoholic fatty liver disease

The association of alcohol with circulating total fibrinogen and plasma clot density is mediated by fibrinogen and FXIII genotypes

The association of alcohol with circulating total fibrinogen and plasma clot density is mediated by fibrinogen and FXIII genotypes

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