Assays In Vitro and In Vivo for Invasion and Metastasis: Application to Drug Testing

Mareel, Marc; Bracke, Marc; Bruyneel, Erik; Larebeke, N. Van; Bourgois, L.; Mets, M. De

doi:10.1007/978-1-349-10671-4_11

Cited by 1 publication

(1 citation statement)

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“…Metastases could be discriminated from pulmonary alveolar adenomas that were solitary, had a more regular architecture and showed hardly any nuclear polymorphism (for illustrations, see Frith and Ward 1-27] and Mareel et al [28]). …”

Section: Tumorigenicity Of Nmumg Cells and Their Derivativesmentioning

confidence: 99%

Morphotypic plasticity in vitro and in nude mice of epithelial mouse mammary cells (NMuMG) displaying an epithelioid (e) or a fibroblastic (f) morphotype in culture

Broecke¹,

Vleminckx²,

Bruyne³

et al. 1996

Clin Exp Metast

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Transition from an epithelioid (e-) to a fibroblastic (f-) morphotype marks invasiveness in clinical and experimental cancer. To understand better the factors influencing such transitions, we have subcloned and manipulated mouse mammary gland (NMuMG) cell cultures and compared the invasive phenotype of multiple subelones in vitro and in vivo. Cell lines with an e-morphotype expressed E-cadberin homogeneously and were not invasive in vitro. Cells with an f-morphotype were E-cadherin-negative and became fully invasive in vitro upon expression of the ras oncogene. Invasive tumors were produced in nude mice after subcutaneous injection of e-type or f-type cells. These tumors showed cystic, glandular and undifferentiated structures. Tumors from f-type cells were E-cadherin-negativewhereas e-type tumors stained heterogeneously in immunohistochemical preparations. Our observations demonstrate the impact of the micro-ecosystem on the invasive phenotype, with in vivo downregulation of E-cadherin and stimulation of the e-to f-morphotype transition.

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Section: Tumorigenicity Of Nmumg Cells and Their Derivativesmentioning

confidence: 99%