Assays for aptamer-based platforms

Citartan, Marimuthu; Gopinath, Subash C.B.; Tominaga, Junji; Tan, Soo-Choon; Tang, Thean‐Hock

doi:10.1016/j.bios.2012.01.002

Cited by 175 publications

(80 citation statements)

References 105 publications

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“…In particular, fluorescence is one of the most frequently used means of evaluating ligand-analyte complex formation in homogeneous and heterogeneous assays, as transduction of the target-binding event facilitated by fluorophores enables real-time monitoring 6 . Surface plasmon (SP) excitation is an appealing way to enhance the fluorescence intensity and sensitivity of the labelled products [7][8][9][10][11][12][13] .…”

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confidence: 99%

An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing

et al. 2013

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Surface plasmon excitation provides stronger enhancement of the fluorescence intensity and better sensitivity than other sensing approaches but requires optimal positioning of a prism to ensure optimum output of the incident light. Here we describe a simple, highly sensitive optical sensing system combining surface plasmon excitation and fluorescence to address this limitation. V-shaped fluidic channels are employed to mimic the functions of a prism, sensing plate, and flow channel in a single setup. Superior performance is demonstrated for different biomolecular recognition reactions on a self-assembled monolayer, and the sensitivity reaches 100 fM for biotin-streptavidin interactions. Using an antibody as a probe, we demonstrate the detection of intact influenza viruses at 0.2 HA units ml À 1 levels. The convenient sensing system developed here has the advantages of being prism-free and requiring less sample (1-2 ml), making this platform suitable for use in situations requiring low sample volumes.

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confidence: 99%

An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing

et al. 2013

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“…Aptamers are single stranded nucleic acid selected in evolutionary process binding to targets with good specificity and binding affinity (Citartan et al, 2012;Feng et al, 2014;Juskowiak, 2011;Kim and Gu 2014;Li et al, 2015;Liu et al, 2009;McKeague and Derosa, 2012). Since the discovery of aptamers in 1990s, aptamer-based assays for small molecules have drawn increasing attentions in environmental sensing, food safety, and clinical analysis due to the unique features of aptamers, such as easy generation, facile labeling, good thermal stability, small size, and target-binding induced structure change (Citartan et al, 2012;Feng et al, 2014;Juskowiak, 2011;Kim and Gu 2014;Li et al, 2015;Liu et al, 2009;McKeague and Derosa, 2012).…”

Section: Introductionmentioning

confidence: 99%

Aptamer fluorescence anisotropy sensors for adenosine triphosphate by comprehensive screening tetramethylrhodamine labeled nucleotides

Zhao

Wang

2015

Biosensors and Bioelectronics

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a b s t r a c tWe previously reported a fluorescence anisotropy (FA) approach for small molecules using tetramethylrhodamine (TMR) labeled aptamer. It relies on target-binding induced change of intramolecular interaction between TMR and guanine (G) base. TMR-labeling sites are crucial for this approach. Only terminal ends and thymine (T) bases could be tested for TMR labeling in our previous work, possibly causing limitation in analysis of different targets with this FA strategy. Here, taking the analysis of adenosine triphosphate (ATP) as an example, we demonstrated a success of conjugating TMR on other bases of aptamer adenine (A) or cytosine (C) bases and an achievement of full mapping various labeling sites of aptamers. We successfully constructed aptamer fluorescence anisotropy (FA) sensors for adenosine triphosphate (ATP). We conjugated single TMR on adenine (A), cytosine (C), or thymine (T) bases or terminals of a 25-mer aptamer against ATP and tested FA responses of 14 TMR-labeled aptamer to ATP. The aptamers having TMR labeled on the 16th base C or 23rd base A were screened out and exhibited significant FA-decreasing or FA-increasing responses upon ATP, respectively. These two favorable TMRlabeled aptamers enabled direct FA sensing ATP with a detection limit of 1 mM and the analysis of ATP in diluted serum. The comprehensive screening various TMR labeling sites of aptamers facilitates the successful construction of FA sensors using TMR-labeled aptamers. It will expand application of TMR-G interaction based aptamer FA strategy to a variety of targets.

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“…As a new platform technology, aptamer-based techniques bioassay has recently been adopted in food analysis, clinical, medical and environmental monitoring [80][81][82][83][84][85]. In food safety monitoring, aptamers are applied for detection of chemical and/or biological hazards.…”

Section: Application Of Aptamer In Food Safetymentioning

confidence: 99%

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

Ndikubwimana¹

2015

J Bioprocess Biotech

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Assays for aptamer-based platforms

Cited by 175 publications

References 105 publications

An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing

An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing

Aptamer fluorescence anisotropy sensors for adenosine triphosphate by comprehensive screening tetramethylrhodamine labeled nucleotides

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

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