Assay of the Capillary Action of Flavanoid Compounds in Mice

Abstract

A method is presented for the assay of biologically active flavanoids by measurement of the reduction which these compounds cause in the sensitivity of the skin capillaries of mice to histamine. The assay is economical of time, space, and apparatus. Limits of error may be calculated from internal evidence. Time/effect relationships are also given for the capillary actions of rutin and hisperidin and for some of their derivatives. The assay methodardescribed heremn e loys mce, skin flap was resected and turned over so that the and may be regarded as a modification of the inner side lay uppermost. The depth of the blue rabbit method of Last and Loew (1947) for the colour was scored for each injection site by reference assessment of antihistamine activity. The advan-to a colour density chart. The total score for each

Some Inhibitors of Histamine‐induced and Formaldehyde‐induced Inflammation in Mice

“…of 0.9% sodium chloride was made into ventral abdominal skin. The procedure was that of Lockett & Jarman (1958), with the difference that we did not anaesthetize the animal to give the histamine. Not only is this more convenient, but it removes the objection that the anaesthesia may alter capillary permeability, as in fact was demonstrated by Lockett & Jarman (1958).…”

“…The procedure was that of Lockett & Jarman (1958), with the difference that we did not anaesthetize the animal to give the histamine. Not only is this more convenient, but it removes the objection that the anaesthesia may alter capillary permeability, as in fact was demonstrated by Lockett & Jarman (1958). After 30 min the mice were killed, the ventral abdominal skin reflected, and the intensity of the blue dye leakage on the inside of the skin noted.…”

“…Lockett & Jarman (1958) have devised a satisfactory method for relating the response quantitatively to an intradermal injection of histamine in the mouse.…”

A New Method for Extraction of Extravasated Dye in the Skin and the Influence of Fasting Stress on Passive Cutaneous Anaphylaxis in Guinea Pigs and Rats*

“…Parratt and West used simple visual grading of the response (19), and Frigeni et al determined it by photometric evaluation (4) . Then, Lockett and Jarman evaluated the intensity of the color of the blue spot by comparing it with a series of comparator cards (11). However, these methods (4,9,11,13,17,19) are not as satisfactory as the method by which the extracted dye is determined as an absolute amount.…”

“…Then, Lockett and Jarman evaluated the intensity of the color of the blue spot by comparing it with a series of comparator cards (11). However, these methods (4,9,11,13,17,19) are not as satisfactory as the method by which the extracted dye is determined as an absolute amount. Therefore, methods for dye extraction have A part of this study was presented at the 48th General Meeting of the Japanese Pharmacological Society, Kobe, Japan, April 8-10, 1975. been reported by many investigators.…”