Assay of Specific Anti-Chlamydia pneumoniae Antibodies by ELISA Method

Kishimoto, Tadamitsu; Kubota, Yoshifumi; Matsushima, Toshiharu; Izutsu, Hiroshi; Matsumoto, Akira; Soejima, Rinzō; Numazaki, Kei; Chiba, Shunzo; Yamazaki, Tsutomu; Sasaki, Nozomu; Kaku, Mitsuo; Shimada, Jingoro; Iwasaki, Eisaku; Baba, Minoru; KOORI, Yoshiaki; Aihara, Masanori; Chikumi, Hiroki; KOSABA, Satoru; Nonaka, Yukiko; Ouchi, Kazunobu; Yamamoto, Tetsuro; Kashiwagi, Seizaburo; Kawayama, Tomotaka; Ohizumi, Kohtaro; Nagai, H.; Nasu, Masaru; Kouno, Shigeru; Tanaka, Hironori; Hirakata, Yoichi; Tateyama, Masao; Saito, Atsushi

doi:10.11150/kansenshogakuzasshi1970.70.830

Cited by 37 publications

(47 citation statements)

References 10 publications

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Section: Discussionmentioning

confidence: 99%

“…In Japan, the ELISA-based detection of C. pneumoniaespecific immunoglobulin G, A and M antibodies (Ab-IgG, Ab-IgA and Ab-IgM) is generally used to identify this pathogen (5,6). In the HITAZYME assay, C. pneumoniae infection is strongly suggested by a value of>3.0 ID for C. pneumoniae Ab-IgG or Ab-IgA in a single serum sample (6).…”

Section: Discussionmentioning

confidence: 99%

“…In the HITAZYME assay, C. pneumoniae infection is strongly suggested by a value of>3.0 ID for C. pneumoniae Ab-IgG or Ab-IgA in a single serum sample (6). A diagnosis of current infection is defined by a rise of at least 1.35 ID in the Ab-IgG level or 1.0 ID in the AbIgA level upon comparison of pairs of serum samples (6). However, since the levels of C. pneumoniae-specific IgG and IgA do not increase during the early stages of infection, making an early diagnosis of primary infection with C. pneumoniae is difficult (2,5,6).…”

Section: Discussionmentioning

confidence: 99%

“…The only sensitive and specific method for detecting this bacterium is assays for C. pneumoniae-specific IgG, IgA and IgM antibodies. Notably, antibody detection also allows the clinician to discriminate between primary infection, reinfection and past exposure (4)(5)(6).…”

Section: Discussionmentioning

confidence: 99%

“…Diagnosing C. pneumoniae infection is often difficult because antibody assays are the only effective diagnostic methods (1,2,4). In Japan, ELISA-based detection of C. pneumoniae-specific immunoglobulin A, G and M antibodies (Ab-IgA, Ab-IgG and Ab-IgM) is generally used to identify this pathogen (5,6). Pneumonia caused by C. pneumoniae usually has a prolonged but mild clinical course (1-3).…”

Section: Introductionmentioning

confidence: 99%

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Two Cases of Acute Respiratory Distress Syndrome with High Values of <i>Chlamydophila pneumoniae</i>-Specific Antibodies

et al. 2013

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We herein report two cases of acute respiratory distress syndrome (ARDS) with high values of Chlamydophila pneumoniae-specific antibodies. In the first case (a 65-year-old man), high levels of anti-C. pneumoniae antibodies (IgG and IgA) were detected on admission, and the anti-C. pneumoniae IgA level rose by Day 30. The patient was successfully treated with quinolone and steroids. In the second case (an 85-year-old man), abnormally high levels of anti-C. pneumoniae IgM were detected on admission. The patient did not recover, despite receiving treatment with several antibiotics and anti-inflammatory agents. Neither of the patients displayed other pathogen-specific antigens or antibodies. Chlamydophila pneumonia is usually mild, although it can cause severe interstitial pneumonia and ARDS in reinfected patients and the elderly.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Two Cases of Acute Respiratory Distress Syndrome with High Values of <i>Chlamydophila pneumoniae</i>-Specific Antibodies

et al. 2013

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ELISA test to detect Chlamydophila pneumoniae IgG

Gutiérrez

Mendoza

Férnandez

et al. 2002

J. Basic Microbiol.

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A new ELISA test (Chlamydophila pneumoniae IgG, Vircell, Spain) to detect Chlamydophila pneuoniae IgG was evaluated. The micro‐immunofluorescence (MIF) test was used as reference method. Chlamydia trachomatis and Chlamydophila psittaci elementary bodies were also assayed. Two hundred and sixteen sera were included in the study: 66 from patients with peripheral arterial occlusive disease (Panel 1), 68 from adults with pneumonia (Panel 2), 44 from healthy adults (Panel 3) and 38 from patients with a sexuality transmitted disease by C. trachomatis (Panel 4). In Panel 1, 51 sera (77%) had antibody titres between 32 and 128; 4 out of 15 sera with IgG titres <32 were positive by ELISA test and 2 sera with 32 IgG titres were uncertain by ELISA; the remaining 60 sera were correctly classified, giving a 91% concordance between the techniques. In Panel 2, 55 sera (81%) had IgG titres between 32 and 512; 2 out of 13 sera with IgG titres <32 were positive by ELISA and 2 sera with 32 titres were uncertain by ELISA; the remaining 64 sera were correctly classified, giving a 97% concordance. In Panel 3, 22 sera (50%) had IgG titres between 32 and 64; only 1 out of 22 sera with IgG titres <32 was positive by ELISA, giving a 97% concordance between the techniques. In Panel 4, there were 24 (63%) negative, 10 (26%) uncertain and 4 (10%) positive results by ELISA, giving an 86% concordance. The C. pneumoniae ELISA test demonstrated 100% sensitivity and 85% specificity. The IgG ELISA test demonstrated a good concordance with the MIF test without the drawbacks associated with the latter assay. We conclude that the ELISA test could be an alternative to the MIF test.

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Presence of Chlamydia pneumoniae in patients with and without atherosclerosis

Podsiadły¹,

Przyłuski

Kwiatkowski

et al. 2005

Eur J Clin Microbiol Infect Dis

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Data published over the past decade show that Chlamydia pneumoniae is likely associated with the development of atherosclerosis. The aim of this study was to ascertain whether C. pneumoniae infections occur more frequently in patients with atherosclerosis than in healthy subjects. A total of 517 persons were studied. Serum samples, leukocytes, and tissue samples were assayed for the presence of C. pneumoniae-specific IgG and IgA antibodies and C. pneumoniae DNA. C. pneumoniae DNA was found in renal, iliac, and brachial vessels, but it was not detected in radial arteries. C. pneumoniae DNA was found most often in directional coronary atherectomy tissue specimens (11/41, 26.8%), but it was also found in the leukocytes of 14.9% (28/188) of patients with atherosclerosis and 24.6% (28/114) of patients without atheroma changes in vessels. Specific IgG and IgA antibodies were present in 63.8 and 49.9% of atheroma patients, respectively. The prevalence of C. pneumoniae antibodies differs significantly in patients with and without atherosclerosis (for IgG, p=0.002, and for IgA, p=0.006). The identification of persons with chlamydial infection of atherosclerotic arteries necessitates the examination of vascular tissues obtained during revascularization procedures. Serological investigation alone cannot identify individuals with vascular chlamydial infections. Detection of C. pneumoniae DNA in peripheral blood mononuclear cells does not seem to be the exclusive marker of persistent vascular infection. A more easily accessible parameter that allows prediction of chlamydial vascular infection is required.

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Assay of Specific Anti-Chlamydia pneumoniae Antibodies by ELISA Method

Cited by 37 publications

References 10 publications

Two Cases of Acute Respiratory Distress Syndrome with High Values of <i>Chlamydophila pneumoniae</i>-Specific Antibodies

Two Cases of Acute Respiratory Distress Syndrome with High Values of <i>Chlamydophila pneumoniae</i>-Specific Antibodies

ELISA test to detect Chlamydophila pneumoniae IgG

Presence of Chlamydia pneumoniae in patients with and without atherosclerosis

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