Assay of Human Thyroid Glands for Thyrocalcitonin Activity1

Aliapoulios, Menelaos A.; Voelkel, Edward F.; Munson, Paul L.

doi:10.1210/jcem-26-8-897

Cited by 96 publications

(27 citation statements)

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“…The magnitude of the Ca decrease in our cases was small when compared with that induced in rats by the electrocauterization of the thyroid (Hirsch et al, 1964). The concentration of thyrocalcitonin was reported much lower in the human than in the rat thyroid, although information is still scanty with the diseased thyroid (Aliapoulios et al, 1966). The metabolic activity of bone appears to determine the sensitivity of the given animals to thyrocalcitonin (Copp, 1967), and this activity is considered less in our patients as compared with the young rats on a low Ca diet.…”

contrasting

confidence: 66%

“…A similar substance which induced hypocalcemia in rat was also extracted from the human thyroid (Aliapoulios et al, 1966). Furthermore, the hog thyrocalcitonin was shown to reduce the blood Ca level but not the blood P level in hypercalcemic patients (Milhaud and Job, 1966 Six patients, 1male and 5 females, and aged from 23 to 37years, were the subjects of this study.…”

mentioning

confidence: 99%

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Transient Fall in Blood Calcium Level Following Thyroid Operations

et al. 1968

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contrasting

confidence: 66%

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confidence: 99%

Transient Fall in Blood Calcium Level Following Thyroid Operations

et al. 1968

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“…Thyrocalcitonin has been found in high concentration in the thyroid of several subjects with pseudohypoparathyroidism (34)(35)(36), but this finding is a manifestation of hypocalcemia (37,38), not hypersecretion of the hormone. Removal of the thyroid glands from two patients with pseudohypoparathyroidism caused only a transient rise in serum calcium in one patient (36) and no detectable change in the other (39).…”

Section: Discussionmentioning

confidence: 99%

Pseudohypoparathyroidism: defective excretion of 3′,5′-AMP in response to parathyroid hormone

Chase¹,

Melson²,

Aurbach³

1969

J. Clin. Invest.

467

158

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A B S T R A C T Urinary excretion of cyclic adenosine 3',5'-monophosphate (3',5'-AMP) was tested in normal subjects and patients with pseudohypoparathyroidism, idiopathic hypoparathyroidism, surgical hypoparathyroidism, and pseudopseudohypoparathyroidism under basal conditions and after a 15 min infusion of purified parathyroid hormone. Basal excretion of the nucleotide was less than normal in the patients with hypocalcemic disorders and greater than normal in pseudopseudohypoparathyroidism. Parathyroid hormone caused a marked increase in excretion of 3',5'-AMP in all subjects except those with pseudohypoparathyroidism; nine patients with this disorder did not respond to the hormone and four showed a markedly deficient response. Radioimmunoassay showed that parathyroid hormone circulated in increased amounts in plasma from patients with pseudohypoparathyroidism and became undetectable when serum calcium was increased above 12 mg/100 ml. Suppression of parathyroid hormone secretion by induction of hypercalcemia did not alter the deficient response to exogenous hormone. The results indicate that: (a) parathyroid hormone circulates in abnormally high concentrations in pseudohypoparathyroidism and secretion of the hormone responds normally to physiological control by calcium; (b) testing urinary excretion of 3',5'-AMP in response to infusion of purified parathyroid hormone appears to-be an accurate and sensitive index for establishing the diagnosis of pseudohypoparathyroidism; and (c) the metabolic defect of the disorder can be accounted for by a lack of or defective form of parathyroid hormone-sensitive adenyl cyclase in bone and kidney.

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“…During the second admission, a biopsy of the thyroid gland was performed. Biological assay of the thyrocalcitonin content in the thyroid tissue according to the method of Aliapoulios et al (1966) revealed no significant increase as compared with normal subjects (8 mMRC units/g gland). The thyrocalcitonin content in peripheral venous plasma which was determined according to the method of Kumar et al (1965) also gave normal result (180 mMRC units/L).…”

Section: Physical Examinationmentioning

confidence: 73%