Assay of Gamma-Glutamyl Transpeptidase Activity in Amniotic Fluid Offers a Possible Prenatal Diagnosis of Biliary Atresia in the Rat Model

Abstract

Muller et al. analysed gamma‐glutamyl transpeptidase (GGTP) activity in the amniotic fluid of more than 2000 pregnant women for a prenatal diagnosis. They reported that at 18–19 weeks' gestation, two fetuses associated with lower amniotic fluid GGTP levels were diagnosed after birth as having biliary atresia (BA). If low GGTP values correlate closely with BA, chemical assay of amniotic fluid GGTP could possibly be used in the prenatal diagnosis of BA. A fetal model of cholestasis in the rat was created by the administration of the toxic cytopharmacological agent phalloidin on day 15 of gestation, after which amniotic fluid was aspirated and analysed for GGTP. Fetal liver specimens were examined histopathologically. In the normal rats, amniotic fluid GGTP values rose rapidly after 18 days 8 h, reaching a maximum value at 19 days of gestation. Significantly lower GGTP values were observed in the cholestasis models between 18 days 16 h and 19 days 16 h of gestation (P<0·05). Our data corroborate Muller et al.'s suggestion that fetuses with cholestasis might demonstrate lower GGTP values in their amniotic fluid at a given stage of gestation. Prenatal diagnosis of BA using the amniotic fluid GGTP assay therefore has considerable potential. © 1997 by John Wiley & Sons, Ltd.

Esophageal, duodenal, rectoanal and biliary atresia, intestinal malrotation, malformed/hypoplastic pancreas, and hypospadias: Further evidence of a new distinct syndrome

“…Because extrahepatic biliary atresia is present in all reported cases, we suggest that amniotic fluid ␥-glutamyltranspeptidase biochemical testing could be used in association with ultrasound studies for prenatal diagnosis in high risk families [Muller et al, 1991;Hosoda et al, 1997].…”

The Sea Lamprey as an Etiological Model for Biliary Atresia

“…Drug administration during pregnancy can induce experimental BA in some animal models [ 58 , 59 ]. A number of postnatal maneuvers (intrabiliary injection of sclerosants and superglue) in a variety of animal models can evoke early neonatal cholestasis [ 60 – 67 ].…”

Aetiology of biliary atresia: what is actually known?

“…Hosada et al administered intraperitoneal phalloidin, an actin-binding toxin derived from the Death Cap mushroom ( Amanita phalloides ) to Wistar rats on gestational day 15. After birth, the pups presented with cholestasis and an increased volume of actin filaments around bile ducts, but without typical BA features [74]. In another study, the anti-helminthic drug, 1,4-phenylenediisothiocyanate, was given orally to pregnant, newborn and adult Wistar rats.…”