Assay of D-dimer based on immunofiltration and staining with gold colloids

Gogstad, Geir O.; Dale, S.; Brosstad, Frank; Brandsnes, Øystein; Holtlund, Jostein; Mørk, Einar; Gärtner, E; Borch, S. M.

doi:10.1093/clinchem/39.10.2070

Cited by 27 publications

(17 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gold colloids produce an intense red staining that is proportional to the amount of D-dimer antigen present. 64 The intensity of the color is then compared with a reference color chart with five different color zones corresponding to D-dimer concentrations of 500, 1000, 2000, 4000 and 8000 ng/mL. 38 SEMINARS IN RESPIRATORY AND CRITICAL CARE MEDICINE-VOL.…”

Section: Nycocard Assaymentioning

confidence: 99%

“…21 There may be interference from rheumatoid factor, cellular material, heparin, or large lipid particles; however, the assay was not affected by bilirubin, hemoglobin, fibrinogen, soluble fibrin, fibrinogen degradation products, or repeated freeze-thawing. 64 The first clinical application of the Nycocard assay in a series of 92 inpatients and outpatients with suspected DVT found it to be 100% sensitive with an NPV of 100%. 21 Several studies done subsequently yielded sensitivities of 85 to 100% and NPV ranging from 0 to 100%.…”

Section: Nycocard Assaymentioning

confidence: 99%

See 1 more Smart Citation

The Role of the D-Dimer in the Diagnosis of Venous Thromboembolism

Ahearn¹,

Bounameaux²

2000

Seminars in Respiratory and Critical Care Medicine

View full text Add to dashboard Cite

Classic enzyme-linked immunosorbent assay (ELISA) D-dimer assays are sensitive in screening for thromboembolic disease; however, they are cumbersome and time consuming to perform, which limits their routine use. Latex agglutination assays are easier to perform, but they are not as sensitive as the ELISA assays. New D-dimer assays incorporating novel technologies can be performed rapidly with a sensitivity approaching that of classic ELISA assays. D-dimer assays are uniformly sensitive in detecting thromboembolic disease in different patient populations; however, low specificity limits the clinical utility of D-dimer measurements in medical inpatients and postoperative patients. Increasingly, these measurements are being incorporated into diagnostic algorithms for venous thromboembolism and are reducing the need for invasive diagnostic studies.

show abstract

Section: Nycocard Assaymentioning

confidence: 99%

Section: Nycocard Assaymentioning

confidence: 99%

The Role of the D-Dimer in the Diagnosis of Venous Thromboembolism

Ahearn¹,

Bounameaux²

2000

Seminars in Respiratory and Critical Care Medicine

View full text Add to dashboard Cite

show abstract

“…In the 1970s a number of epitopes of the late products of thrombolysis were defined in particular fragments DD (D‐Dimer) and DE (fragment E) 29,30,34 . At the same time monoclonal antibodies were becoming widely available allowing these neo‐antigens to be targeted 30,35 .…”

Section: Introductionmentioning

confidence: 99%

“…At the same time monoclonal antibodies were becoming widely available allowing these neo‐antigens to be targeted 30,35 . This led to the development of monoclonal antibodies directed against various epitopes which are only exposed during the late stages of thrombolysis 34,36,37 .…”

Section: Introductionmentioning

confidence: 99%

“…These have suggested that certain D‐Dimer tests (particularly the ELISA tests), have sufficient sensitivity to be useful as a negative predictor for PE and DVT, but are too nonspecific for use as a diagnostic test if positive 27 . In recent times, the search for more rapid and useful clinical tests has continued leading to tests such as SimpliRED and NYCOCARD D‐Dimer 34,37 . The utility of these various D‐Dimer tests is now discussed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A review of the usefulness of D‐Dimer in the diagnosis of pulmonary embolism

Mountain

Brown

1996

Emergency Medicine

View full text Add to dashboard Cite

Pulmonary embolism is a potentially fatal condition that is extremely difficult to accurately diagnose clinically, usually relying on radiological investigations to make the diagnosis. These confirmatory tests are expensive, time consuming and may be associated with considerable morbidity. Thus, the utility of a blood test that reliably makes or refutes the diagnosis is apparent. Most blood tests involve the later stages of thrombolysis. Of these, the D‐Dimer tests are the most sensitive, and when combined with monoclonal antibody technology, are of clinical value. Enzyme‐linked immunosorbent assay D‐Dimer tests have good sensitivity (95%) and moderate specificity (30–45%), but technical considerations prevent their routine use. Latex agglutination tests overcome these technical problems, but their sensitivity is variable (46–100%). Two recently introduced tests show promise. The NYCO‐CARD D‐Dimer test is a semiquantitative modified enzyme‐linked immunosorbent assay test. Relatively few clinical studies have been performed and further validation studies are required. The SimpliRED D‐Dimer test uses a new bispecific antibody technique, allows bed‐side testing, and has good sensitivity (84–100%) and negative predictive values (92–99%). These tests could be used to screen low risk patients, thus avoiding ventilation‐perfusion scans, or in combination with ventilation‐perfusion scans and lower limb studies to avoid angiograms in intermediate probability pulmonary embolism patients. Future research will define where these tests show greatest utility, particularly in which patient subgroups, or symptom time intervals.

show abstract

D-Dimer Testing: Laboratory Aspects and Current Issues

Thachil

Lippi

Favaloro

2017

Methods in Molecular Biology

View full text Add to dashboard Cite

D-dimer has become one of the commonly requested coagulation tests, especially in the venous thromboembolism rule out setting. Appropriate D-dimer testing has significantly decreased the number of radiological investigations in this clinical scenario. D-dimer testing also plays an important role in the diagnostic process of the systemic coagulation disorders, especially disseminated intravascular coagulation in conjunction with other coagulation tests. However, widespread D-dimer testing without good understanding of the technical issues related to the test has recently caused some concerns. This review aims to address the basic physiology of D-dimer formation, the different methods available for its routine assessment, the current problems that both clinicians and laboratory scientists face with this test, and the important need for harmonization of commercial immunoassays.

show abstract

Assay of D-dimer based on immunofiltration and staining with gold colloids

Cited by 27 publications

References 22 publications

The Role of the D-Dimer in the Diagnosis of Venous Thromboembolism

The Role of the D-Dimer in the Diagnosis of Venous Thromboembolism

A review of the usefulness of D‐Dimer in the diagnosis of pulmonary embolism

D-Dimer Testing: Laboratory Aspects and Current Issues

Contact Info

Product

Resources

About