Assay of Chikungunya Virus in Cell Monolayers by Immunofluorescence

Abstract

A linear function was demonstrated between imurofluorescent focus counts and relative virus concentration. The inmunofluorescent assay was comparable in sensitivity to but more precise and rapid than virus assays based on the intracerebral inoculation of suckling mice or on plaque counting. By the immunofluorescence procedure, the 50% neutralizing end point of antiviral serum was rapidly and quantitatively determined.