Assay of adenosine in human adipose tissue

Ranta, Susanna; Kiviluoto, Tuula; Newby, Andrew C.; Ohisalo, Jorma J.

doi:10.1530/acta.0.1100429

Cited by 11 publications

(8 citation statements)

References 10 publications

(12 reference statements)

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“…As far as we know, this study is the first to describe high plasma adenosinelevels inoverweight/obesewithnormal pregnancyduring their third trimester of gestation. These results agree with previous reports in obese adults showing high adenosine levels in adipose tissue homogenates [17]. We also found high plasma adenosine levels in obese children [18].…”

Section: Adenosine In Obesitysupporting

confidence: 94%

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High plasma adenosine levels in overweight/obese pregnant women

Badillo

Salgado

Bravo

et al. 2017

Purinergic Signalling

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We aim to investigate whether overweight/obese pregnant women have elevated plasma levels of adenosine associated with increased consumption of high-calorie food. Sixty women were included. They were divided into lean (n = 23 and n = 12) or overweight/obese (n = 7 and n = 18) non-pregnant and pregnant women, respectively. Clinical records and maternal blood samples were collected after informed consent. A self-reported dietary questionnaire was also completed. Plasma adenosine levels were determined with high-performance liquid chromatography. Biochemical parameters, including glucose, total protein, and lipid profile, were determined using standard colorimetric assays. Adenosine levels were higher in pregnant women than in non-pregnant women (18.7 ± 1.6 vs 10.8 ± 1.3 nM/μg protein, respectively, p < 0.0001). Overweight/obese pregnant women (21.9 ± 2.5 nM/μg protein) exhibited higher adenosine levels than lean pregnant (14.5 ± 1.0 nM/μg protein, p = 0.04) or non-pregnant women (11.7 ± 1.5 nM/μg protein, p = 0.0005). Also, pregnant women with elevated weight gain exhibited higher (26.2 ± 3.7 nM/μg protein) adenosine levels than those with adequate weight gain (14.9 ± 1.4 nM/μg protein, p = 0.03). These differences were not statistically significant compared with those of pregnant women with reduced weight gain (17.4 ± 2.1 nM/μg protein, p = 0.053). Body mass index and adenosine only in pregnant women were positively correlated (r = 0.39, p = 0.02). While, polyunsaturated fatty acid (PUFA) consumption was negatively correlated with plasma adenosine levels only in non-pregnant women (r = -0.33, p = 0.03). Pregnancy is associated with high plasma adenosine levels, which are further elevated in pregnant women who are overweight/obese. High PUFA intake might reduce plasma adenosine levels in non-pregnant women.

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Section: Adenosine In Obesitysupporting

confidence: 94%

“…In this regard, no reports concerning plasma adenosine levels in overweight/obese pregnant women have been published. But, high concentrations of this molecule were found in the homogenates of adipose tissue obtained from obese adults [17] or in plasma from obese children [18].…”

Section: Introductionmentioning

confidence: 96%

High plasma adenosine levels in overweight/obese pregnant women

Badillo

Salgado

Bravo

et al. 2017

Purinergic Signalling

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“…Using this method, amounts of adenosine as low as 1 pmol per sample could be determined. The intra-assay variability was 11.7% (N = 9) and the inter-assay variability 14.9% (N = 5) (Ranta et al 1985).…”

Section: Methodsmentioning

confidence: 95%

“…The tissue pieces, weighing 10-20 mg, were frozen in liquid nitrogen within one second of excision by a conchotome. Adenosine was assayed from pooled samples as described in detail previously (Ranta et al 1985). Briefly, the pieces were first ground in liquid nitrogen and placed in perchloric acid.…”

Section: Methodsmentioning

confidence: 99%

Adenosine and the regional differences in adipose tissue metabolism in women

Stoneham

Kiviluoto²,

Keso³

et al. 1988

Acta Endocrinologica

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Adenosine content in abdominal and femoral adipose tissue in menstruating women was 0.38 \ m=+-\ 0.10 and 0.59 \ m=+-\ 0.14 nmol/g of wet weight, respectively (mean \m=+-\sem; N = 17). No difference in adenosine sensitivity was found between abdominal and femoral adipocytes. In lactating women, the adenosine content was lower in femoral than in abdominal adipose tissue (0.40 \ m=+-\0.08 and 0.57 \ m=+-\0.08 nmol/g of wet weight, respectively; N = 10). Adenosine sensitivity in femoral adipocytes was not increased during lactation. As adenosine is a locally acting insulin-like effector, these results suggest that the higher adenosine content in femoral adipose tissue in menstruating women could explain its higher lipoprotein lipase activity and tendency to accumulate fat. During lactation, the lower extracellular adenosine concentration would allow lipid mobilization preferentially from the femoral site.

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“…In other experiments, 10-50 mg pieces were removed rapidly with tongs precooled in liquid N2. The pieces were ground in liquid N2 and their adenosine contents were assayed by radioimmunoassay as described elsewhere (Ranta et al, 1985).…”

Section: Methodsmentioning

confidence: 99%

Thyroid status and adenosine content of adipose tissue

Ohisalo

Stoneham

Keso

1987

Biochemical Journal

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Fat-cells from hypothyroid rats lack the normal lipolytic response to catecholamines. We have suggested that this is due to increased sensitivity to the inhibitory actions of endogenous adenosine. In this paper we present evidence that thyroid hormones increase adipose-tissue adenosine contents and suggest that the increased sensitivity to adenosine in hypothyroidism is due to relief from desensitization by endogenous adenosine.

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Assay of adenosine in human adipose tissue

Cited by 11 publications

References 10 publications

High plasma adenosine levels in overweight/obese pregnant women

High plasma adenosine levels in overweight/obese pregnant women

Adenosine and the regional differences in adipose tissue metabolism in women

Thyroid status and adenosine content of adipose tissue

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