Assay Guided Comparison for Enzymatic and Non-Enzymatic Antioxidant Activities with Special Reference to Medicinal Plants

Panda, Siva Prasad

doi:10.5772/50782

Cited by 18 publications

(13 citation statements)

References 81 publications

(70 reference statements)

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“…Considering the most recent studies (Alam et al, 2013;Niki, 2010;Panda, 2012), the most commonly in vitro methods used for the determination of antioxidant capacity are reviewed. The assays are based on different mechanisms, aiming to evaluate: (i) the depletion of free radicals by antioxidants: scavenging capacity assays against stable, non-biological, free radicals or against specific reactive oxygen species (ROS)/reactive nitrogen species (RNS); (ii) the capacity of antioxidants to reduce metal ions: ferric reducing antioxidant power (FRAP) and cupric ion reducing antioxidant capacity (CUPRAC); (iii) competitive methods: oxygen radical absorbance capacity (ORAC) and total radical trapping antioxidant parameter (TRAP); (iv) determination of total phenolic and total flavonoid content; (v) reducing power (RP).…”

Section: An Overview Of the Assay Methods Used To Estimate Antioxidanmentioning

confidence: 99%

“…The required knowledge and technical skills also differ among methods. Basically, these assays differ from each other in terms of reagents, substrates, experimental conditions, reaction media and analytical evaluation methods (Alam et al, 2013;Panda, 2012).…”

Section: An Overview Of the Assay Methods Used To Estimate Antioxidanmentioning

confidence: 99%

“…On the other hand, 2,2 -azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) decolorizing assay is applicable for both hydrophilic and lipophilic antioxidants (Alam et al, 2013;Panda, 2012).…”

Section: Scavenging Capacity Assays Against Stable Non-biological Fmentioning

confidence: 99%

“…The inhibition of the generated nitrite oxide is quantified by comparing the absorbance values of control and test samples. Curcumin, caffeic acid, sodium nitrite, butylated hydroxyanisole (BHA), ascorbic acid and rutin are typically used as a positive control (Dasgupta and De, 2007;Magalhães et al, 2008;Panda, 2012).…”

Section: Nitric Oxide Scavenging Activitymentioning

confidence: 99%

“…It is promptly decomposed in oxygen and water, which might produce HO • that can initiate lipid peroxidation and cause DNA damage in the body. The ability of plant extracts to scavenge H 2 O 2 is usually estimated using a buffered (phosphate buffer) solution of H 2 O 2 in mixture with the extract and subsequent evaluation of the absorbance at 230 nm (Alam et al, 2013;Panda, 2012;Ruch et al, 1989).…”

Section: Hydrogen Peroxide Scavenging Assaymentioning

confidence: 99%

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Asteraceae species with most prominent bioactivity and their potential applications: A review

Bessada

Barreira

Oliveira

2015

Industrial Crops and Products

114

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a b s t r a c tOxidative stress has a relevant part in the etiology of several diseases and metabolic disorders, being reasonable to expect that antioxidant compounds might have beneficial effects in health maintenance or disease prevention. Antioxidant compounds might be isolated and characterized from different plant constituents, such as roots, stems, bark, leaves, flowers, fruits and seeds, using proper extraction methods. The Asteraceae family has a worldwide distribution, with special relevance in the Mediterranean, Eastern Europe and Asia Minor, being acknowledged about 25 000 species integrated in approximately 1000 genera. In addition to the anti-inflammatory, analgesic and antipyretic potential of some of these species, their high antioxidant power, as proven in research works with extracts (of roots, stems, bark, leaves, flowers, fruits and seeds) should be highlighted. Herein, the Asteraceae species with highest potential as sources of natural antioxidants with potential uses in medicine and in pharmaceutical, cosmetic and food industries were identified. The species were selected based on their botanical representativeness, being identified the 9 most relevant species: Achillea millefolium L., Acmella oleraceae Murr., Artemisia absinthium L., Bidens pilosa L., Carthamus tinctorius L., Inula crithmoides L., Matricaria recutita L., Otanthus maritimus L. and Parthenium hysterophorus L.. With the obtained information, it could be concluded that the bioactivity of the selected Asteraceae species lacks a complete characterization, constituting a research scope with great potential to be exploited in the development of dietary supplements, bioactive food ingredients or pharmaceutical based products with application in food industry, dermocosmetics or medicine.

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Section: An Overview Of the Assay Methods Used To Estimate Antioxidanmentioning

confidence: 99%

Section: An Overview Of the Assay Methods Used To Estimate Antioxidanmentioning

confidence: 99%

Section: Scavenging Capacity Assays Against Stable Non-biological Fmentioning

confidence: 99%

Section: Nitric Oxide Scavenging Activitymentioning

confidence: 99%

Section: Hydrogen Peroxide Scavenging Assaymentioning

confidence: 99%

See 3 more Smart Citations

Asteraceae species with most prominent bioactivity and their potential applications: A review

Bessada

Barreira

Oliveira

2015

Industrial Crops and Products

114

View full text Add to dashboard Cite

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Tools and Targeted Genes for Plant Disease Detection

Munusamy

Abdullah

2017

Crop Improvement

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Potential of Plant Growth-Promoting Microbes in Disease Reduction by Influencing the Antioxidant Enzymes of Medicinal and Spice Plants

Musheer

Ashraf

Choudhary

et al. 2021

Antioxidants in Plant-Microbe Interaction

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Assay Guided Comparison for Enzymatic and Non-Enzymatic Antioxidant Activities with Special Reference to Medicinal Plants

Cited by 18 publications

References 81 publications

Asteraceae species with most prominent bioactivity and their potential applications: A review

Asteraceae species with most prominent bioactivity and their potential applications: A review

Tools and Targeted Genes for Plant Disease Detection

Potential of Plant Growth-Promoting Microbes in Disease Reduction by Influencing the Antioxidant Enzymes of Medicinal and Spice Plants

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