Assay for urinary desmosines in a healthy pre-pubertal population using an improved extraction technique

Winfield, Kaye; Gard, S.; Kent, G.N.; Sly, Peter D.; Brennan, Siobhain

doi:10.1258/000456306776021571

Cited by 4 publications

(4 citation statements)

References 17 publications

(35 reference statements)

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“…Cumiskey's method was, on its turn, slightly modified by Winfield et al either in sample pretreatment procedures (hydrolysis time and temperature were increased and size of the cellulose column used for DES extraction was enhanced) or in HPLC elution conditions (mobile phase composition was changed following a stepwise protocol). The method was applied to urine of healthy children in the pubertal range, aged 4 weeks to 12 years.…”

Section: Lc‐based Methods For the Determination Of Dess In Biologicalmentioning

confidence: 99%

From micellar electrokinetic chromatography to liquid chromatography‐mass spectrometry: Revisiting the way of analyzing human fluids for the search of desmosines, putative biomarkers of chronic obstructive pulmonary disease

et al. 2013

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Desmosine (DES) and isodesmosine are two isomer amino acids unique-to-mature, cross-linked elastin. Based on this feature, they have been discussed as surrogate markers of chronic obstructive pulmonary disease, a disorder characterized by progressive degradation of lung elastin. Despite the development of numerous protocols, detection of DESs in body fluids is still considered to be technically challenging. In fact, owing to the minute concentration of these circulating cross-links, their accurate measurement may be provided only by sophisticated and sensitive techniques. Aim of this article is to present the "history" of the two techniques (MEKC and LC-MS) that, better than others, allowed scientists to "bring their best to the table" in this area. Both of them meet the criteria of (almost) complete automation of the procedure and of the use of more selective and sensitive detection systems. The substantial advantages in terms of precision and accuracy provided by such measurements suggest that the science of DESs is eventually catching up with its promise and the assumption that these candidate biomarkers can be associated to clinical variables holds true.

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Section: Lc‐based Methods For the Determination Of Dess In Biologicalmentioning

confidence: 99%

From micellar electrokinetic chromatography to liquid chromatography‐mass spectrometry: Revisiting the way of analyzing human fluids for the search of desmosines, putative biomarkers of chronic obstructive pulmonary disease

et al. 2013

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“…Standard doses of IV antibiotics were used and blood levels were monitored where appropriate. Each subject provided a spot urine sample16, 20 and blood sample, and pulmonary function tests (PFTs) within 72 hr of the initiation of IV antibiotics, on day 3–9 and on day 7–12 of therapy. Sputum samples were collected at similar time‐points on a subset of patients who could spontaneously expectorate, the results of which have been reported previously 18.…”

Section: Methodsmentioning

confidence: 99%

Urinary desmosine: A biomarker of structural lung injury during CF pulmonary exacerbation

Laguna

Wagner

Starcher

et al. 2012

Pediatric Pulmonology

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Summary Rationale Cystic fibrosis (CF) lung disease is characterized by structural changes and remodeling in airway architecture and lung parenchyma. Neutrophilic inflammation and infection lead to injury and breakdown of airway matrix constituents, including elastin. The non-invasive measurement of urinary desmosine (UDes), a breakdown product of elastin, may be reflective of ongoing lung injury and may serve as a biomarker of active short-term damage during pulmonary exacerbation. Our objectives were to measure desmosine in the urine of CF patients hospitalized for treatment of a pulmonary exacerbation and to explore the correlation between desmosine concentration and other markers of clinical improvement, including lung function and inflammatory mediators. Methods Urine and blood samples plus lung function measurements were collected at up to three points during hospitalization for treatment of a CF pulmonary exacerbation. We used a repeated measures model, adjusted for age and time between measurements, to compare log transformed urine desmosine concentrations across multiple time points and to correlate those concentrations with related clinical variables. Change in UDes concentration was investigated using a statistical model that incorporated normalization factors to account for variations in urinary concentration. Results Desmosine was measured by radioimmunoassay in 155 spot urine samples from 53 CF patients hospitalized for 63 pulmonary exacerbations (range of results: 0–235 pmol Des/mL). Specific gravity adjusted UDes concentration decreased significantly during admission for CF pulmonary exacerbation, p<0.01 (average length of stay = 11 days). No correlation was observed between UDes concentration and lung function or inflammatory markers. Conclusions Urinary desmosine decreased significantly following treatment for an acute pulmonary exacerbation and may be a useful biomarker of short-term injury to the CF lung. Further investigation is needed to evaluate the utility of UDes concentration in the long-term progression of CF lung disease.

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“…By contrast, the concentration of Des and Ides were significantly lower in the formalin-fixed yellow ligament compared to the frozen samples, indicating that they were not preserved by this treatment and suggesting that formalin may mask or alter elastin crosslinks. To obtain a diagnostic tool applicable on children (aged 4 wk to 12 years), Winfield et al [87] have produced a method in which the extraction technique of urinary desmosines was improved in comparison with the procedures previously published. This goal was achieved by increasing hydrolysis time, temperature, and cellulose column size and separating hydrolysate on RP-HPLC.…”

Section: Hplcmentioning

confidence: 99%

Progress in the methodological strategies for the detection in real samples of desmosine and isodesmosine, two biological markers of elastin degradation

Viglio

Annovazzi

Luisetti

et al. 2007

J of Separation Science

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Desmosines are crosslinking amino acids unique to mature elastin in humans. Owing to this unicity, they have been discussed as potentially attractive indicators of connective tissue disorders whose clinical manifestations are mostly the result of elastin degradation. This review covers advances in immunochemical, chromatographic, and electrophoretic procedures applied in the last 25 years to detect and quantitate these crosslinksin a variety of biological samples. Recent applications of CE with LIF detection (CE-LIF) for investigating the content of desmosines in different fluids will also be discussed.

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Assay for urinary desmosines in a healthy pre-pubertal population using an improved extraction technique

Cited by 4 publications

References 17 publications

From micellar electrokinetic chromatography to liquid chromatography‐mass spectrometry: Revisiting the way of analyzing human fluids for the search of desmosines, putative biomarkers of chronic obstructive pulmonary disease

From micellar electrokinetic chromatography to liquid chromatography‐mass spectrometry: Revisiting the way of analyzing human fluids for the search of desmosines, putative biomarkers of chronic obstructive pulmonary disease

Urinary desmosine: A biomarker of structural lung injury during CF pulmonary exacerbation

Progress in the methodological strategies for the detection in real samples of desmosine and isodesmosine, two biological markers of elastin degradation

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