Assay for fluorescein diacetate hydrolytic activity: Optimization for soil samples

Green, V. Steven; Stott, Diane E.; Diack, Mateugue

doi:10.1016/j.soilbio.2005.06.020

Cited by 541 publications

(287 citation statements)

References 16 publications

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“…In NT soil, the values of their measurements of concentration of released fluorescein were 14% and 30% higher in relation to soil under tillage systems based on chisel and mouldboard plough, respectively. FDA hydrolysis is carried out by active cells with a variety of enzymes as proteases, lipases and esterases, therefore, it is considered to be a suitable index of the overall enzymes and microbial activity in soil (Green et al, 2006). Tillage practices can affect both the physical and chemical properties of the soil dynamically in space and time, and have consequences on soil biological activity and soil functions.…”

Section: Resultsmentioning

confidence: 99%

Effects of different soil management practices on soil properties and microbial diversity

Gajda¹,

Czyż²,

Dexter³

et al. 2018

International Agrophysics

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The effects of different tillage systems on the properties and microbial diversity of an agricultural soil was investigated. In doing so, soil physical, chemical and biological properties were analysed in 2013-2015, on a long-term field experiment on a loamy sand at the IUNG-PIB Experimental Station in Grabów, Poland. Winter wheat was grown under two tillage treatments: conventional tillage using a mouldboard plough and traditional soil tillage equipment, and reduced tillage based on soil crushing-loosening equipment and a rigid-tine cultivator. Chopped wheat straw was used as a mulch on both treatments. Reduced tillage resulted in increased water content throughout the whole soil profile, in comparison with conventional tillage. Under reduced tillage, the content of readily dispersible clay was also reduced, and, therefore, soil stability was increased in the toplayers, compared with conventional tillage. In addition, the beneficial effects of reduced tillage were reflected in higher soil microbial activity as measured with dehydrogenases and hydrolysis of fluorescein diacetate, compared with conventional tillage. Moreover, the polimerase chain reaction – denaturing gradient gel electrophoresis analysis showed that soil under reduced till-age had greater diversity of microbial communities, compared with conventionally-tilled soil. Finally, reduced tillage increased organic matter content, stability in water and microbial diversity in the top layer of the soil.

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Section: Resultsmentioning

confidence: 99%

Effects of different soil management practices on soil properties and microbial diversity

Gajda¹,

Czyż²,

Dexter³

et al. 2018

International Agrophysics

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“…A atividade enzimática foi expressa em µg N-NH + 4 g -1 2 h -1 . A hidrólise do diacetato de fluoresceína (FDA) foi determinada pelo método de Green et al (2006), com algumas alterações descritas na sequência. Foram incubadas, em triplicatas, amostras de 1 g de solo com 20 mL de tampão fosfato de sódio 60 mmol L -1 a 25 °C durante 15 min sob agitação a 100 rpm.…”

Section: Methodsunclassified

Indicadores Microbiológicos de Solo em Pastagem com Aplicação Sucessiva de Dejetos De Suínos

Silva

Antoniolli²,

Jacques³

et al. 2015

Rev. Bras. Ciênc. Solo

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RESUMO O uso de dejetos líquidos de suínos como fertilizante do solo é uma prática comum na Região Sul do Brasil. Apesar de ter benefícios na reutilização dos dejetos, essa prática apresenta sérios riscos ambientais. Os indicadores microbiológicos de qualidade do solo são bastante sensíveis e permitem o monitoramento das condições do ambiente edáfico. Objetivou-se avaliar a qualidade microbiológica do solo de pastagens com diferentes históricos de uso sucessivo de dejetos líquidos de suínos. Determinaram-se o teor de C da biomassa microbiana, a respiração microbiana do solo, o quociente metabólico (qCO2) e a atividade das enzimas β-glicosidase, urease e hidrólise do diacetato de fluoresceína (FDA), em áreas de pastagem com uso de dejetos de suínos há dois anos (A2) e 14 anos (A14) e em área com mata nativa (MN). O uso sucessivo de dejetos de suínos em pastagem não influenciou o C da biomassa e a respiração microbiana do solo, que variaram conforme a época de coleta. O qCO2 não foi influenciado pelo uso de dejetos de suínos no solo; a atividade enzimática do solo foi influenciada pelo uso de dejetos de suínos, sendo que a urease e a FDA foram sensíveis na detecção de diferenças na atividade dos solos com uso de dejetos de suínos, enquanto a β-glicosidase não permitiu a diferenciação entre as áreas estudadas.

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“…FDA hydrolysis was analysed according to a protocol from Green et al (2006). Mean FDA hydrolysis rate per plot was calculated as mg fluorescein per g soil per 3 h.…”

Section: Sequence Data Availabilitymentioning

confidence: 99%

Spatial patterns of microbial diversity and activity in an aged creosote-contaminated site

et al. 2014

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Restoration of polluted sites via in situ bioremediation relies heavily on the indigenous microbes and their activities. Spatial heterogeneity of microbial populations, contaminants and soil chemical parameters on such sites is a major hurdle in optimizing and implementing an appropriate bioremediation regime. We performed a grid-based sampling of an aged creosote-contaminated site followed by geostatistical modelling to illustrate the spatial patterns of microbial diversity and activity and to relate these patterns to the distribution of pollutants. Spatial distribution of bacterial groups unveiled patterns of niche differentiation regulated by patchy distribution of pollutants and an east-to-west pH gradient at the studied site. Proteobacteria clearly dominated in the hot spots of creosote pollution, whereas the abundance of Actinobacteria, TM7 and Planctomycetes was considerably reduced from the hot spots. The pH preferences of proteobacterial groups dominating in pollution could be recognized by examining the order and family-level responses. Acidobacterial classes came across as generalists in hydrocarbon pollution whose spatial distribution seemed to be regulated solely by the pH gradient. Although the community evenness decreased in the heavily polluted zones, basal respiration and fluorescein diacetate hydrolysis rates were higher, indicating the adaptation of specific indigenous microbial populations to hydrocarbon pollution. Combining the information from the kriged maps of microbial and soil chemistry data provided a comprehensive understanding of the long-term impacts of creosote pollution on the subsurface microbial communities. This study also highlighted the prospect of interpreting taxa-specific spatial patterns and applying them as indicators or proxies for monitoring polluted sites.

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Assay for fluorescein diacetate hydrolytic activity: Optimization for soil samples

Cited by 541 publications

References 16 publications

Effects of different soil management practices on soil properties and microbial diversity

Effects of different soil management practices on soil properties and microbial diversity

Indicadores Microbiológicos de Solo em Pastagem com Aplicação Sucessiva de Dejetos De Suínos

Spatial patterns of microbial diversity and activity in an aged creosote-contaminated site

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