Assay for Enterotoxin in Fecal Specimens of Clostridium perfringens Food Poisoning

Itoh, Takeshi; Inaba, Misako; Saito, Kosuke; Sakai, Senzo; Uemura, Takashi; Sakaguchi, Genji

doi:10.11150/kansenshogakuzasshi1970.53.409

Cited by 3 publications

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“…An important point for the diagnosis of C. perfringens food poisoning is that CPE were detected in feces of patients within 3 days of the onset in many food poisoning outbreaks (16). However, CPE was not detected in all patients' feces within 3 days of the onset.…”

Section: Discussionmentioning

confidence: 99%

Four Foodborne Disease Outbreaks Caused by a New Type of Enterotoxin-Producing Clostridium perfringens

et al. 2015

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The epidemiological and bacteriological investigations on four foodborne outbreaks caused by a new type of enterotoxinproducing Clostridium perfringens are described. C. perfringens isolated from patients of these outbreaks did not produce any known enterotoxin and did not carry the C. perfringens enterotoxin gene. However, the culture filtrates of these isolates induced the accumulation of fluid in rabbit ileal loop tests. The molecular weight of the new enterotoxin may be between 50,000 and 100,000, although the known C. perfringens enterotoxin is ca. 35,000. This new enterotoxin was heat labile, and its biological activities were inactivated by heating for 5 min at 60°C. The new enterotoxin was sensitive to pH values higher than 11.0 and protease treatment but was resistant to trypsin treatment. These results suggest that the new enterotoxin may be a protein. Although C. perfringens enterotoxin induced morphological changes in Vero cells, the changes induced by the new enterotoxin differed from those by the known C. perfringens enterotoxin. The new enterotoxin also induced morphological changes in L929 cells, whereas the known C. perfringens enterotoxin did not, because L929 cells lacked an appropriate enterotoxin receptor. Although C. perfringens enterotoxin is recognized as the only diarrheagenic toxin responsible for C. perfringens foodborne outbreaks, the results of the present study indicate that C. perfringens isolated from these four outbreaks produced a new type of enterotoxin.

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Section: Discussionmentioning

confidence: 99%

Four Foodborne Disease Outbreaks Caused by a New Type of Enterotoxin-Producing Clostridium perfringens

et al. 2015

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Excretion of Enterotoxin-Producing Clostridium perfringens in Feces by Patients during and after Diarrhea

Saito¹

1991

kansenshogakuzasshi

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The excretion in the feces of Clostridium perfringens producing enterotoxin by 48 patients in 3 cases of food poisoning was studied during the illness and 1 and 3 months after the onset. Patients in the first outbreak excreted such strains mostly in the form of spores. In the second outbreak, spores and viable count cells were excreted in nearly equal numbers, and about 50% of the spores were enterotoxigenic. In the third outbreak, the strains were detected both as spores and as viable count cells during the illness. In all three groups, most fecal samples contained at least 10(3) spores per gram. About 1 month after the onset of the illness, (long after recovery from symptoms), 12 colonies producing enterotoxin were detected from five of the 22 samples of feces obtained from subjects in the three groups. There were few C. perfringens organisms, mostly spores, in the feces. Enterotoxin was not detected in the feces. About 3 months after onset, colonies producing enterotoxin could not be detected from feces of 11 of the subjects in the first group. We conclude that persons affected by C. perfringens food poisoning might contaminate food for at least 1 month after the onset.

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Rapid and Specific Detection of Enterotoxin-Producing Clostridium perfringens at an Early Stage of Sporulation by Flow Cytometry.

Piyankarage

Uemura

2000

Jpn.J.Food Microbiol.

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Assay for Enterotoxin in Fecal Specimens of Clostridium perfringens Food Poisoning

Cited by 3 publications

References 0 publications

Four Foodborne Disease Outbreaks Caused by a New Type of Enterotoxin-Producing Clostridium perfringens

Four Foodborne Disease Outbreaks Caused by a New Type of Enterotoxin-Producing Clostridium perfringens

Excretion of Enterotoxin-Producing Clostridium perfringens in Feces by Patients during and after Diarrhea

Rapid and Specific Detection of Enterotoxin-Producing Clostridium perfringens at an Early Stage of Sporulation by Flow Cytometry.

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