Assay for Acetazolamide in Plasma

Quantitation of Acetazolamide in Plasma by High-Performance Liquid Chromatography

“…Bayne et al (6) had a similar problem with their acetazolamide assay. In its absence, the peak height ratio of the drug to the internal standard diminished on successive injections of plasma extract, which may have been due to an accumulation of interfering substances.…”

“…Methods for the quantitation of this drug in biological fluids include measurement of the carbonic anhydrase inhibition (1, a), colorimetry (3,4), electron-capture GLC (5), and high-performance liquid chromatography (HPLC) (6). Methods for the quantitation of this drug in biological fluids include measurement of the carbonic anhydrase inhibition (1, a), colorimetry (3,4), electron-capture GLC (5), and high-performance liquid chromatography (HPLC) (6).…”

Validated Stability indicating Spectrophotometric Method for the Determination of Acetazolamide in Dosage Forms through Complex Formation with Palladium (II)

“…It acts on the central nervous system to retard the abnormal, paroxysmal, and excessive discharge from CNS neurons (1). Various methods were reported for its determination either per se or in pharmaceutical preparations and biological fluids including: spectrophotometry (2)(3)(4)(5)(6), HNMR (4), electrochemical methods (7,8), and HPLC (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27). The British Pharmacopoeia (BP) (28) recommended potentiometric determination of ACM in dimethylformamide using ethanolic solution of sodium hydroxide as a titrant, while the United States Pharmacopoeia (USP) method (29) recommended a spectrophotometric measurement of the acidic solution of the pharmaceutical preparation at 265 nm.…”

High-Pressure Liquid Chromatographic Analysis of Drugs in Biological Fluids II: Determination of an Antiarrhythmic Drug, Tocainide, as Its Dansyl Derivative Using a Fluorescence Detector

“…The microgram or nanogram per milliliter concentrations of many drugs found in plasma after therapeutic doses have confined the use of HPLC analysis to drugs having high molar absorptivities at 254 or 280 nm, the wavelengths most frequently used in these detectors (12)(13)(14)(15)(16)(17)(18). UV absorption detectors have been commonly used in HPLC.…”