Assay evaluability of drug testing systems determined with human renal carcinoma cell lines

Kurth, K. H.; Dongen, Jan Willem Van; Romijn, J. A.; Lieber, Michael M.; Schröder, Fritz H.

doi:10.1007/bf00328096

Cited by 10 publications

(3 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The three tumor lines, RC-8, RC-9, and NC-65, have all been derived from patients with renal cell carcinomas in the advanced stage and have been maintained in nude mice (12)(13)(14)(15). During subsequent passages, no alterations occurred in the growth kinetics, histology, or immunohistochemistry of the tumors.…”

Section: Tumor Linesmentioning

confidence: 99%

The Role of Interleukin-6 in the Induction of Hypercalcemia in Renal Cell Carcinoma Transplanted into Nude Mice

et al. 1997

View full text Add to dashboard Cite

The role of interleukin-6 in the induction of hypercalcemia in renal cell carcinoma transplanted into nude mice Weissglas, M.G.; Schamhart, D.H.J.; Lowik, C; Papapoulos, S.; Theuns, H.; Kurth, K-H. Published in: Endocrinology DOI:10.1210/en.138.5.1879 Link to publication Citation for published version (APA):Weissglas, M. G., Schamhart, D. H. J., Lowik, C., Papapoulos, S., Theuns, H., & Kurth, K-H. (1997). The role of interleukin-6 in the induction of hypercalcemia in renal cell carcinoma transplanted into nude mice. Endocrinology, 138, 1879-1885. DOI: 10.1210/en.138.5.1879 General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. ABSTRACTHypercalcemia is a well known complication of renal cell carcinoma (RCC). As RCCs can produce IL-6, and IL-6 may stimulate bone resorption and cause mild hypercalcemia, we examined whether IL-6 is involved in renal cancer-associated hypercalcemia in vivo. Three human renal cell carcinoma tumor lines (RC-8, RC-9, and NC-65) growing in nude mice were studied. Tumors were implanted sc, and parameters of bone metabolism and serum human IL-6 levels were determined in relation to tumor volume (TV). All three tumor lines secreted human IL-6, although in different quantities. The maximum level of IL-6 in RC-8 was 434 pg/ml (TV, 200 mm 3 ), that in RC-9 was 81 pg/ml (TV, 1800 mm 3 ), and that in NC-65 was 2368 pg/ml (TV, 1800 mm 3 ). Hypercalcemia developed in RC-8 and RC-9 tumor-bearing animals, but not in NC-65-bearing animals. The hypercalcemia in both RC-8 and RC-9 tumor lines was associated with elevated levels of PTH-related peptide (PTHrP) and loss of trabecular bone volume.Serum calcium and phosphate concentrations showed an almost linear relationship with plasma PTHrP independently of the tumor line and serum IL-6 levels. No hypercalcemia occurred in the NC-65 animals, which had the highest levels of IL-6, but no detectable plasma PTHrP and PTHrP messenger RNA expression in the tumor. Administration of neutralizing antibodies to IL-6 to RC-8 animals normalized serum calcium concentrations and PTHrP values and induced a significant inhibition of tumor growth. No such effect on tumor growth of anti-IL-6 was seen in the other two tumor lines. The normalization of serum calcium in RC-8 mice is most likely attributed to the growthinhibit...

show abstract

Section: Tumor Linesmentioning

confidence: 99%

The Role of Interleukin-6 in the Induction of Hypercalcemia in Renal Cell Carcinoma Transplanted into Nude Mice

et al. 1997

View full text Add to dashboard Cite

show abstract

“…Therefore, we suspect that biological differences in the subfractions of RCT may give rise to differences in behavior of tumor metastases, and undoubtedly have consequences for chemotherapy. The development of model systems for further cell biological and experimental investigations of RCT has led to the establishment of 4 human renal-cell xenografts in nude mice (Kurth et al, 1984) and of a tumor line from a rat RCT in Wistar Lewis rats (deVere White et al, 1980). In this study, we present findings on the 2-parameter FCM analysis of cellular DNA and 'In these cases labelling with either cytokeratin or vimentin antibodies resulted in the detection of an aneuploid peak not seen without labelling.…”

mentioning

confidence: 99%

Tissue‐specific markers in flow cytometry of urological cancers. II. Cytokeratin and vimentin in renal‐cell tumors

Feitz

Karthaus

Beck

et al. 1986

Intl Journal of Cancer

View full text Add to dashboard Cite

Nine primary human renal-cell tumors (RCT), one lymph-node metastasis, 4 human xenografts of a RCT in nude mice and a rat RCT line were analyzed by flow cytometry (FCM) using propidium iodide for DNA analysis and antibodies to cytokeratin and vimentin in the indirect immunofluorescence technique for labelling of specific tumor-cell populations. By means of 2-dimensional FCM analysis, vimentin- and cytokeratin-positive (tumor) cells were compared and their DNA content and proliferative fraction analyzed separately from those of cytokeratin-negative stromal and inflammatory cells. In primary human RCT, 2 subpopulations of cells were detected and analyzed separately. Small numbers of tumor cells with an abnormal DNA stemline were also detected. In addition, co-expression of intermediate filament proteins of both the cytokeratin and the vimentin types was detected in the aneuploid cell population. Comparison of 2 model systems of RCT with primary human RCT revealed a similar pattern of tumor-cell subfractions within these tumors. The 2-parameter FCM analysis permits the detection of subpopulations in complex cell suspensions and the quantification of these fractions, as well as analysis of their cellular DNA content.

show abstract

“…Since IFN-alpha appears to be a potential active antitumor agent in patients with RCC [20,21] and TNF also shows very clear in vitro cytostatic and cytolytic effects on human renal cell carcinoma cell lines, we decided to test the in vitro antiproliferative effects of human recombinant IFN-alpha and -gamma as well as human recombinant TNF on two RCC tumor lines-NC-65 and RC-43 [22,23].…”

Section: Introductionmentioning

confidence: 99%

Effect of alpha‐ and gamma‐Interferon and tumor necrosis factor on colony formation of two human renal tumor xenografts in vitro

Beniers

Peelen

Hendriks

et al. 1988

Seminars in Surgical Oncology

View full text Add to dashboard Cite

The in vitro antitumor activity of alpha- and gamma-interferon as well as tumor necrosis factor was tested on two human renal cell carcinoma xenografts--RC-43 and NC-65. A dose-dependent inhibition of colony formation in soft agar was found for both tumor lines. NC-65, however, showed no sensitivity for gamma-interferon. Combination drug tests showed a synergistic effect on colony formation of both tumor lines. The strongest inhibition was shown by the combination of alpha-interferon and tumor necrosis factor. We conclude that both interferons as well as tumor necrosis factor show direct in vitro cytostatic effects against renal tumor direct in vitro cytostatic effects against renal tumor cells.

show abstract

Assay evaluability of drug testing systems determined with human renal carcinoma cell lines

Cited by 10 publications

References 19 publications

The Role of Interleukin-6 in the Induction of Hypercalcemia in Renal Cell Carcinoma Transplanted into Nude Mice

The Role of Interleukin-6 in the Induction of Hypercalcemia in Renal Cell Carcinoma Transplanted into Nude Mice

Tissue‐specific markers in flow cytometry of urological cancers. II. Cytokeratin and vimentin in renal‐cell tumors

Effect of alpha‐ and gamma‐Interferon and tumor necrosis factor on colony formation of two human renal tumor xenografts in vitro

Contact Info

Product

Resources

About