ASR1 transcription factor and its role in metabolism

Domínguez, Pía Guadalupe; Carrari, Fernando

doi:10.4161/15592324.2014.992751

Cited by 33 publications

(36 citation statements)

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“…Moreover, the levels of Trp, which is considered to be a modulator of defense ( Ward et al, 2010 ), were clearly higher in the treated plants. As previously described ( Dominguez and Carrari, 2015 ), higher Thr and Trp levels could be a consequence of the observed increase in ASR1 expression in the treated plants. As expected, 1-MT levels were also higher in the treated plants.…”

Section: Discussionsupporting

confidence: 73%

1-Methyltryptophan Modifies Apoplast Content in Tomato Plants Improving Resistance Against Pseudomonas syringae

et al. 2018

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Plants can produce numerous natural products, many of which have been shown to confer protection against microbial attack. In this way, we identified 1-methyltryptophan (1-MT), a natural compound produced by tomato plants in response to Pseudomonas syringae attack, whose application by soil drench provided protection against this pathogen. In the present work, we have studied the mechanisms underlying this protection. The results demonstrated that 1-MT can be considered a new activator of plant defense responses that acts by inhibiting the stomatal opening produced by coronatine (COR) and could thereby, prevent bacteria entering the mesophyll. Besides, 1-MT acts by blocking the jasmonic acid (JA) pathway that, could avoid manipulation of the salicylic acid (SA) pathway by the bacterium, and thus hinder its growth. Although the concentration of 1-MT reached in the plant did not show antimicrobial effects, we cannot rule out a role for 1-MT acting alone because it affects the expression of the fliC gene that is involved in synthesis of the flagellum. These changes would result in reduced bacterium motility and, therefore, infective capacity. The results highlight the effect of a tryptophan derivative on induced resistance in plants.

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Section: Discussionsupporting

confidence: 73%

1-Methyltryptophan Modifies Apoplast Content in Tomato Plants Improving Resistance Against Pseudomonas syringae

et al. 2018

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“…Second, to identify other factors involved in ASR gene expression, sucrose (100 m m ) and its analogue, turanose (100 m m ), ABA (50 μ m ) and its inhibitor, NDGA (100 μ m ), IAA (50 μ m ), methyl jasmonic acid (MeJA, 50 μ m ) and ethephon (100 μ m ) were used to treat strawberry LG (13 days)‐stage (Figure a) and tomato MG (33 days)‐stage (Figure b) fruits (Dominguez and Carrari, ). These results showed that in tomato and strawberry fruits, both sucrose and ABA induced the ASR gene expression levels, and sucrose + ABA remarkably promoted the ASR expression.…”

Section: Resultsmentioning

confidence: 99%

Abscisic acid and sucrose regulate tomato and strawberry fruit ripening through the abscisic acid‐stress‐ripening transcription factor

Jia

Jiu

Zhang

et al. 2016

Plant Biotechnology Journal

201

154

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SummaryAlthough great progress has been made towards understanding the role of abscisic acid (ABA) and sucrose in fruit ripening, the mechanisms underlying the ABA and sucrose signalling pathways remain elusive. In this study, transcription factor ABA‐stress‐ripening (ASR), which is involved in the transduction of ABA and sucrose signalling pathways, was isolated and analysed in the nonclimacteric fruit, strawberry and the climacteric fruit, tomato. We have identified four ASR isoforms in tomato and one in strawberry. All ASR sequences contained the ABA stress‐ and ripening‐induced proteins and water‐deficit stress‐induced proteins (ABA/WDS) domain and all ASR transcripts showed increased expression during fruit development. The expression of the ASR gene was influenced not only by sucrose and ABA, but also by jasmonic acid (JA) and indole‐3‐acetic acid (IAA), and these four factors were correlated with each other during fruit development. ASR bound the hexose transporter (HT) promoter, which contained a sugar box that activated downstream gene expression. Overexpression of the ASR gene promoted fruit softening and ripening, whereas RNA interference delayed fruit ripening, as well as affected fruit physiological changes. Change in ASR gene expression influenced the expression of several ripening‐related genes such as CHS,CHI, F3H,DFR,ANS,UFGT,PG,PL,EXP1/2,XET16, Cel1/2 and PME. Taken together, this study may provide new evidence on the important role of ASR in cross‐signalling between ABA and sucrose to regulate tomato and strawberry fruit ripening. The findings of this study also provide new insights into the regulatory mechanism underlying fruit development.

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“…418This low level of phylogenetic divergence within LP3 can also generally be observed in the 419 other two domain-based trees (N-terminal tree and C-terminal tree). The N-terminal tree 420 focused on the putative zinc binding domains in the N-terminal region of ASR/LP3 genes, 421 therefore it would make sense that there is a low level of divergence occurring since ASR/LP3 422 requires Zinc ions to adopt their functional conformation(Dominguez and Carrari, 2015; 423 González and Iusem, 2014). A lack of clear segregation between ASR and LP3 can also be 424 indicative of high purifying selective pressures thereby favouring a conserved nucleotide 425 sequence.…”

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confidence: 99%

Evolutionary analysis of LP3 gene family in conifers: an ASR homolog

García‐Gil

Lecoy

2020

Preprint

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8Drought has long been established as a major environmental stress for plants which have in turn 9 developed several coping strategies, ranging from physiological to molecular mechanisms. 10 LP3; a homolog of the Abscisic Acid, Stress and Ripening (ASR) gene was first detected in 11 tomato; and has been shown to be present in four different isoforms in loblolly pine called LP3-12 0, LP3-1, LP3-2 and LP3-3. While ASR has already been extensively studied notably in tomato, 13 the same cannot be said of LP3. Like ASR, the different LP3 isoforms have been shown to be 14 upregulated in response to water deficit stress and to also act as transcription factors for genes 15 likely involved in hexose transport. In this study we have investigated the evolutionary history 16 of LP3 gene family, with the aim of relating it to that of ASR from a phylogenetic perspective 17 and comparing the differences in selective pressure and codon usage. Phylogenetic analyses of 18 different LP3 homologs compared to ASR show that LP3 is less divergent across species than 19 ASR and that even when comparing the different sub-sections of the gene the divergence rate 20 of LP3 is lower than that of ASR. Analysis of different gene parameters showed that there were 21 differences in GC1% and GC2% but not in total or GC3% content. All genes had a relatively 22 high CAI value associated with a low to moderate ENC value, which is indicative of high 23 translation efficiency found in highly expressed genes. Analysis of codon usage also showed 24 that LP3 preferentially uses different codons than ASR. Selective pressure analysis across most 25 of the LP3 and ASR genes used in this study showed that these genes were principally 26 undergoing purifying selection, with the exception of LP3-3 which seems to be undergoing 27 diversifying selection most probably due to the fact that it likely recently diverged from LP3-28 0. This study thus provides insight in how ASR and LP3 have diverged from each other while 29 remaining homologous. 30

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ASR1 transcription factor and its role in metabolism

Cited by 33 publications

References 50 publications

1-Methyltryptophan Modifies Apoplast Content in Tomato Plants Improving Resistance Against Pseudomonas syringae

1-Methyltryptophan Modifies Apoplast Content in Tomato Plants Improving Resistance Against Pseudomonas syringae

Abscisic acid and sucrose regulate tomato and strawberry fruit ripening through the abscisic acid‐stress‐ripening transcription factor

Evolutionary analysis of LP3 gene family in conifers: an ASR homolog

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