ASPP 092, a phenolic diarylheptanoid from Curcuma comosa suppresses experimentally-induced inflammatory ear edema in mice

Abstract

Curcuma comosa Roxb., family Zingiberaceae, exhibits diverse biological activities. This study was aimed to investigate the anti-inflammatory potential of a major phenolic diarylheptanoid isolated from C. comosa , ASPP 092 [(3S)-1-(3,4-dihydroxy-phenyl)-7-phenyl-(6E)-6-hepten-3-ol] in an experimentally-induced inflammatory ear edema model in mice. Ear edema in the mice was induced by the topical application of irritant, ethyl phenylpropiolate (EPP). The topical application of ASPP 092 at the edema site was directed immediately after the EPP application. The edematous responses were assessed at different time points by measuring the thickness of each ear before and after the EPP application followed by histopathology analysis. The expressions of major inflammatory cytokines were analyzed by real-time RT-PCR followed by the immunohistochemistry analysis of cyclooxygenase (COX-2). The topical application of ASPP 092 effectively suppressed the EPP-induced edematous formation in the ear of mice. Histopathological analysis showed substantial improvements in epidermal hyperplasia and inflammatory cell infiltration. ASPP 092 treatment also modulated the expressions of inflammatory cytokines including Tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-1β (IL-1β), and Matrix metalloproteinase-13 (MMP-13). The expressions of cyclooxygenases (COX) including COX-1 and COX-2 were significantly reduced by ASPP 092 treatment. For the first time, our results suggest the efficacy of ASPP 092 to suppress experimentally-induced inflammation in a preclinical model in mice; however, a more detailed evaluation of its mechanism of action is necessary before evaluating its efficacy and safety in randomized trials.

Green synthesis of selenium nanoparticles capped by Tithonia diversifolia pectin for anti‐inflammation activity

“…EPP-induced ear edema is an acute inflammatory response model frequently used in experimental animal studies. 15,24 TA B L E 2 The anti-inflammatory activity of PT-SeNPs treated group compared to negative and positive control groups. Table 2 showed that the ears treated with PT-SeNPs reduced considerably edema thickness at all time intervals after induction of EPP compared to the no-treatment ears.…”

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“…In this context, the higher dose of CEBc was active against the acute inflammation caused by sub-plantar injection of carrageenan in a weaker intensity as induced by Indomethacin. Carrageenan-induced paw edema in the mouse is also associated with nociceptive changes in the paw and migration of inflammatory cells in the injection site and is sensitive to non-steroidal antiinflammatory drugs 29 . It has been proved lately that the phase of the inflammatory response after the injection of carrageenan in the paw of the mice is associated with the production of the cyclooxygenase 2 (COX-2), high prostaglandin production resulting from cyclooxygenases 1 and 2 (COX-1 and COX-2) activities, free oxygenated radicals like NO (nitric oxide) produced by endothelial and inducible nitric oxide synthase (eNOS and iNOS) and neutrophil infiltration 30,32 .…”