Aspirin promotes bone marrow mesenchymal stem cell-based calvarial bone regeneration in mini swine

Cao, Yu; Xiong, Jie; Mei, Shenghui; Wang, Fu; Zhao, Zhigang; Wang, Songlin; Liu, Yi

doi:10.1186/s13287-015-0200-4

Cited by 69 publications

(55 citation statements)

References 31 publications

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“…In addition to its long-term application as an antipyretic and analgesic for postoperative pain relief [21], ASP is also reported to substantially improve immunomodulatory function, manifested as regulatory T cells upregulation and Th17 cells downregulation in vitro [22]. In addition, systemic use of ASP revealed enhanced bone regeneration in vivo [23]. Moreover, some scholars have recently demonstrated that low doses of ASP can regulate the balance between bone resorption and bone formation in osteoporosis caused by ovariectomy [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Icariin/Aspirin Composite Coating on TiO2 Nanotubes Surface Induce Immunomodulatory Effect of Macrophage and Improve Osteoblast Activity

You

Chen

et al. 2020

Coatings

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Surface coating modification of titanium-based alloys is an efficient way to accelerate early osseointegration in dental implant fields. Icariin (ICA) is a traditional Chinese medicine that has bone activating functions, while aspirin (ASP) is a classical non-steroidal anti-inflammatory drug with good antipyretic and analgesic capabilities. Moreover, poly(lactic–co–glycolic acid) (PLGA) has attracted great attention due to its excellent biocompatibility and biodegradability. We superimposed an ASP/PLGA coating onto ICA loaded TiO2 nanotubes structure so as to establish an icariin/aspirin composite coating on TiO2 nanotubes surface. Scanning electron microscopy, X-ray photoelectron spectroscopy, a contact angle test and a drug release test confirmed the successful preparation of the NT–ICA–ASP/PLGA substrate, with a sustained release pattern of both ICA and ASP. Compared to those cultured on the Ti surface, macrophage cells on the NT-ICA-ASP/PLGA substrate displayed decreased M1 proinflammatory and enhanced M2 proregenerative genes and proteins expression, which implied activated immunomodulatory effect. Moreover, when cultured with conditioned medium from macrophages, osteoblast cells on the NT-ICA-ASP/PLGA substrate revealed improved cell proliferation, adhesion and osteogenic genes and proteins expression, compared with those on the Ti surface. The abovementioned results suggest that the established NT-ICA-ASP/PLGA substrate is a promising candidate for functionalized coating material in Ti implant surface modification.

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Section: Introductionmentioning

confidence: 99%

Icariin/Aspirin Composite Coating on TiO2 Nanotubes Surface Induce Immunomodulatory Effect of Macrophage and Improve Osteoblast Activity

You

Chen

et al. 2020

Coatings

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“…19 Moreover, a low dose of aspirin can promote BMSCs' proliferation. 44 The ACS-CCM prepared in this study showed satisfactory cytocompatibility and could promote the adhesion and proliferation of BMSCs ( Figure 5). In addition, the number of cells on the 50 mg ACS-CCM samples was considerably higher than that on the other membranes.…”

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confidence: 73%

Guided bone regeneration with asymmetric collagen-chitosan membranes containing aspirin-loaded chitosan nanoparticles

Zhang¹,

Ma²,

Liu³

et al. 2017

IJN

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Introduction Membranes allowing the sustained release of drugs that can achieve cell adhesion are very promising for guided bone regeneration. Previous studies have suggested that aspirin has the potential to promote bone regeneration. The purpose of this study was to prepare a local drug delivery system with aspirin-loaded chitosan nanoparticles (ACS) contained in an asymmetric collagen-chitosan membrane (CCM). Methods In this study, the ACS were fabricated using different concentrations of aspirin (5 mg, 25 mg, 50 mg, and 75 mg). The drug release behavior of ACS was studied. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to examine the micromorphology of ACS and aspirin-loaded chitosan nanoparticles contained in chitosan-collagen membranes (ACS-CCM). In vitro bone mesenchymal stem cells (BMSCs) were cultured and critical-sized cranial defects on Sprague-Dawley rats were made to evaluate the effect of the ACS-CCM on bone regeneration. Results Drug release behavior results of ACS showed that the nanoparticles fabricated in this study could successfully sustain the release of the drug. TEM showed the morphology of the nanoparticles. SEM images indicated that the asymmetric membrane comprised a loose collagen layer and a dense chitosan layer. In vitro studies showed that ACS-CCM could promote the proliferation of BMSCs, and that the degree of differentiated BMSCs seeded on CCMs containing 50 mg of ACS was higher than that of other membranes. Micro-computed tomography showed that 50 mg of ACS-CCM resulted in enhanced bone regeneration compared with the control group. Conclusion This study shows that the ACS-CCM would allow the sustained release of aspirin and have further osteogenic potential. This membrane is a promising therapeutic approach to guiding bone regeneration.

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“…There are only a few reports on the effects of ASA on stem cell differentiation. With its inhibiting effect on tumor necrosis factor‐α (TNF‐α) and interferon‐γ (IFN‐γ) production, aspirin has been shown to promote the osteogenesis of bone MSC in vitro, improve calvarial bone repair in vivo (Cao et al, ), and limit ectopic bone formation caused by BMP‐2 treatment (Subramanian et al, ). While there are contradicting results on the effect of ASA on cell proliferation, there are reports showing that aspirin can enhance osteogenic and cardiomyocyte differentiation of bone marrow‐derived MSC (Hao, Shi, Zhou, Wang, & Nie, ).…”

Section: Discussionmentioning

confidence: 99%

“…There are only a few reports on the effects of ASA on stem cell differentiation. With its inhibiting effect on tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) production, aspirin has been shown to promote the osteogenesis of bone MSC in vitro, improve calvarial bone repair in vivo (Cao et al, 2015), and limit ectopic bone formation caused by BMP-2 treatment (Subramanian et al, 2015). (Alcaraz et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Vascular tissue engineering: Fabrication and characterization of acetylsalicylic acid‐loaded electrospun scaffolds coated with amniotic membrane lysate

Aslani

Kabiri

Kehtari

et al. 2019

Journal Cellular Physiology

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As the incidence of small‐diameter vascular graft (SDVG) occlusion is considerably high, a great amount of research is focused on constructing a more biocompatible graft. The absence of a biocompatible surface in the lumen of the engineered grafts that can support confluent lining with endothelial cells (ECs) can cause thrombosis and graft failure. Blood clot formation is mainly because of the lack of an integrated endothelium. The most effective approach to combat this problem would be using natural extracellular matrix constituents as a mimic of endothelial basement membrane along with applying anticoagulant agents to provide local antithrombotic effects. In this study, we fabricated aligned and random electrospun poly‐L‐lactic acid (PLLA) scaffolds containing acetylsalicylic acid (ASA) as the anticoagulation agent and surface coated them with amniotic membrane (AM) lysate. Vascular scaffolds were structurally and mechanically characterized and assessed for cyto‐ and hemocompatibility and their ability to support endothelial differentiation was examined. All the scaffolds showed appropriate tensile strength as expected for vascular grafts. Lack of cytotoxicity, cellular attachment, growth, and infiltration were proved using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay and scanning electron microscopy. The blood compatibilities of different scaffolds examined by in vitro hemolysis and blood coagulation assays elucidated the excellent hemocompatibility of our novel AM‐coated ASA‐loaded nanofibers. Drug‐loaded scaffolds showed a sustained release profile of ASA in 7 days. AM‐coated electrospun PLLA fibers showed enhanced cytocompatibility for human umbilical vein ECs, making a confluent endothelial‐like lining. In addition, AM lysate‐coated ASA‐PLLA‐aligned scaffold proved to support endothelial differentiation of Wharton's jelly‐derived mesenchymal stem cells. Our results together indicated that AM lysate‐coated ASA releasing scaffolds have promising potentials for development of a biocompatible SDVG.

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Aspirin promotes bone marrow mesenchymal stem cell-based calvarial bone regeneration in mini swine

Cited by 69 publications

References 31 publications

Icariin/Aspirin Composite Coating on TiO2 Nanotubes Surface Induce Immunomodulatory Effect of Macrophage and Improve Osteoblast Activity

Icariin/Aspirin Composite Coating on TiO2 Nanotubes Surface Induce Immunomodulatory Effect of Macrophage and Improve Osteoblast Activity

Guided bone regeneration with asymmetric collagen-chitosan membranes containing aspirin-loaded chitosan nanoparticles

Vascular tissue engineering: Fabrication and characterization of acetylsalicylic acid‐loaded electrospun scaffolds coated with amniotic membrane lysate

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