Aspirin is an efficient inhibitor of quorum sensing, virulence and toxins in Pseudomonas aeruginosa

El-Mowafy, Somaia A.; Galil, Khaled H. Abd El; El‐Messery, Shahenda M.; Shaaban, Mona I.

doi:10.1016/j.micpath.2014.07.008

Cited by 153 publications

(107 citation statements)

References 49 publications

Supporting

Mentioning

100

Contrasting

Order By: Relevance

“…This rationale has been used in previous studies. As a result, a number of useful quorum sensing inhibitors have been found among FDA approved drugs such as niclosamide, aspirin and diclofenac sodium [26][27][28] .…”

Section: Discussionmentioning

confidence: 99%

Glyceryl trinitrate is a novel inhibitor of quorum sensing in <i>Pseudomonas aeruginosa</i>

Abbas

Shaldam

2017

Afr H. Sci.

View full text Add to dashboard Cite

Background: Targeting quorum sensing is an alternative approach to antibiotics.Targeting quorum sensing-regulated virulence will disarm the pathogen without exerting pressure on its growth. As a result, emergence of resistance is avoided and the immune system can easily eradicate bacteria. Objectives: Investigation of the possible inhibition of quorum sensing-regulated virulence of Pseudomonas aeruginosa by glyceryltrinitrate. Methods: The quorum sensing inhibiting activity of glyceryl trinitrate was assessed by inhibition of violacein production in Chromobacterium violaceum ATCC 12472. Its ability to inhibit pyocyanin, protease, biofilm and tolerance to oxidative stress was evaluated. Docking study was performed to study the interference of glyceryl trinitrate with the binding of autoinducers with LasR and rhlR receptors. Results: Glyceryl trinitrate exerted a significant biofilm inhibiting and eradicating activities. It decreased the production of quorum-sensing dependent violacein production. It significantly inhibited the production of pyocyanin and protease and diminished the tolerance against oxidative stress. Molecular docking study showed that glyceryl trinitrate interferes with the binding of autoinducers to their receptors. It could bind to Las Rand rhlr receptors with binding energy of -93.47 and -77.23, respectively. Conclusion: Glyceryl trinitrate can be an antivirulence agent in the treatment of Pseudomonas aeruginosa topical infections such as burn infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Glyceryl trinitrate is a novel inhibitor of quorum sensing in <i>Pseudomonas aeruginosa</i>

Abbas

Shaldam

2017

Afr H. Sci.

View full text Add to dashboard Cite

show abstract

“…SAL at therapeutic levels decreases biofilm production by Candida spp. (21,(32)(33)(34), E. coli (25,26,35,36), P. aeruginosa (37)(38)(39), and S. epidermidis (Table 2) (30,38,40). Biofilm production by Salmonella enterica serovar Typhimurium was also reduced by SAL, but the concentrations tested were not specified (41).…”

mentioning

confidence: 99%

“…Biofilm production by Salmonella enterica serovar Typhimurium was also reduced by SAL, but the concentrations tested were not specified (41). One study showed no effect of SAL on biofilm production by E. coli (37).…”

mentioning

confidence: 99%

Antimicrobial Effects of Antipyretics

Zimmermann

Curtis

2017

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Antipyretics are some of the most commonly used drugs. Since they are often coadministered with antimicrobial therapy, it is important to understand the interactions between these two classes of drugs. Our review is the first to summarize the antimicrobial effects of antipyretic drugs and the underlying mechanisms involved. Antipyretics can inhibit virus replication, inhibit or promote bacterial or fungal growth, alter the expression of virulence factors, change the surface hydrophobicity of microbes, influence biofilm production, affect the motility, adherence, and metabolism of pathogens, interact with the transport and release of antibiotics by leukocytes, modify the susceptibility of bacteria to antibiotics, and induce or reduce the frequency of mutations leading to antimicrobial resistance. While antipyretics may compromise the efficacy of antimicrobial therapy, they can also be beneficial, for example, in the management of biofilm-associated infections, in reducing virulence factors, in therapy of resistant pathogens, and in inducing synergistic effects. In an era where it is becoming increasingly difficult to find new antimicrobial drugs, targeting virulence factors, enhancing the efficacy of antimicrobial therapy, and reducing resistance may be important strategies.

show abstract

“…RMSD profile showed that throughout the simulation, LasR-cyanidin complex is more stable than the LasRsignaling molecule complex. In a recent study Mowafy et al (2014) evidenced that docking analysis may suggest the quorum quenching efficiency of test compounds. It was proved through molecular docking studies that aspirin can act as an anti-quorum agent against P. aeruginosa.…”

Section: Molecular Dynamics Simulationmentioning

confidence: 99%

“…So far, several natural and chemically synthesized compounds have been demonstrated to have the QSI activity. This includes malvidin (Gopu et al 2015), aspirin (Mowafy et al 2014), eugenol (Zhou et al 2013), ellagic acid (Sarabhai et al 2013) and, rutin (Vikram et al 2010). On the other hand, most of these compounds have not been used in the mammalian system because of their adverse physiological activity and toxicity.…”

Section: Introductionmentioning

confidence: 99%

Cyanidin inhibits quorum signalling pathway of a food borne opportunistic pathogen

Gopu

Shetty

2016

J Food Sci Technol

View full text Add to dashboard Cite

Quorum sensing (QS) is the process of population dependent cell to cell communication used by bacteria to regulate their phenotypic characteristics. Key virulence factors that determine the bacterial pathogenicity and food spoilage were found to be regulated by QS mechanism. Hence, disrupting the QS signaling pathway could be an attractive strategy to manage food borne pathogens. In the current study, QS inhibitory activity of a naturally occurring anthocyanincyanidin and its anti-biofilm property were assessed against an opportunistic pathogen Klebsiella pneumoniae, using a biosensor strain. Further, QS inhibitory property of a naturally occurring anthocyanin cyanidin was further confirmed using in-silico techniques like molecular docking and molecular dynamics simulation studies. Cyanidin at sub-lethal dose significantly inhibited QS-dependent phenotypes like violacein production (73.96 %), biofilm formation (72.43 %), and exopolysaccharide production (68.65) in a concentrationdependent manner. Cyanidin enhanced the sensitivity of test pathogen to conventional antibiotics in a synergistic manner. Molecular docking analysis revealed that cyanidin binds more rigidly with LasR receptor protein than the signaling compound with a docking score of −9.13 Kcal/mol. Molecular dynamics simulation predicted that QS inhibitory activity occurs through the conformational changes between the receptor and cyanidin complex. Our results indicate that cyanidin, can be a potential QS based antibiofilm and antibacterial agent for food borne pathogens.

show abstract

Aspirin is an efficient inhibitor of quorum sensing, virulence and toxins in Pseudomonas aeruginosa

Cited by 153 publications

References 49 publications

Glyceryl trinitrate is a novel inhibitor of quorum sensing in <i>Pseudomonas aeruginosa</i>

Glyceryl trinitrate is a novel inhibitor of quorum sensing in <i>Pseudomonas aeruginosa</i>

Antimicrobial Effects of Antipyretics

Cyanidin inhibits quorum signalling pathway of a food borne opportunistic pathogen

Contact Info

Product

Resources

About