Asperosaponin VI, A Saponin Component from <i>Dipsacus asper</i> Wall, induces Osteoblast Differentiation through Bone Morphogenetic Protein‐2/p38 and Extracellular Signal‐regulated Kinase 1/2 Pathway

Niu, Youguo; Li, Yuhua; Huang, Haitao; Kong, Xianghe; Zhang, Rong; Liu, Li; Sun, Yang; Wang, Tingmei; Mei, Qibing

doi:10.1002/ptr.3414

Cited by 75 publications

(46 citation statements)

References 29 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These compounds can be divided into 6 categories, including flavonoids: icariin (Figure 1) [23–26], genistein (Figure 2) [29], daidzein (Figure 2) [31, 32], kaempferol (Figure 11) [72], quercetin (Figure 11) [73, 74], naringin (Figure 8) [75–77], hesperidin (Figure 11) [78], linarin (Figure 11) [79], bavachalcone (Figure 3) [36], rutin (Figure 11) [80], (+)-catechin (Figure 11) [81], nobiletin (Figure 11) [82], luteolin (Figure 11) [83], baicalein (Figure 11) [84], baicalin (Figure 1) [85], xanthohumol (Figure 11) [86]; coumarins: psoralen (Figure 3) [35], osthole (Figure 11) [87]; lignans: honokiol (Figure 11) [88, 89], isotaxiresinol (Figure 11) [90], magnolol (Figure 11) [91]; polyphenol: resveratrol (Figure 11) [92–95], curcumin (Figure 11) [96–98], tea polyphenols (including epigallocatechin-3-gallate, epigallocatechin, epi-catechin, epicatechin-3-gallate, Figure 11) [99, 100]; anthraquinones: rubiadin (Figure 10), 2-hydroxy-1-methoxy-anthraquinone (Figure 10), 1,3,8-trihydroxy-2-methoxy-anthraquinone (Figure 10) [71]; alkaloids: harmine (Figure 11) [101], coptisine (Figure 11) [102], palmatine (Figure 11) [103], berberine (Figure 11) [104, 105]; and other compounds: curculigoside (Figure 11) [106, 107], asperosaponin VI (Figure 11) [108], limonoid 7-oxo-deacetoxygeduni...…”

Section: Resultsmentioning

confidence: 99%

Potential Antiosteoporotic Agents from Plants: A Comprehensive Review

Jia

Nie

Cao

et al. 2012

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Osteoporosis is a major health hazard and is a disease of old age; it is a silent epidemic affecting more than 200 million people worldwide in recent years. Based on a large number of chemical and pharmacological research many plants and their compounds have been shown to possess antiosteoporosis activity. This paper reviews the medicinal plants displaying antiosteoporosis properties including their origin, active constituents, and pharmacological data. The plants reported here are the ones which are commonly used in traditional medical systems and have demonstrated clinical effectiveness against osteoporosis. Although many plants have the potential to prevent and treat osteoporosis, so far, only a fraction of these plants have been thoroughly investigated for their physiological and pharmacological properties including their mechanism of action. An attempt should be made to highlight plant species with possible antiosteoporosis properties and they should be investigated further to help with future drug development for treating this disease.

show abstract

Section: Resultsmentioning

confidence: 99%

Potential Antiosteoporotic Agents from Plants: A Comprehensive Review

Jia

Nie

Cao

et al. 2012

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…The membranes were washed three times for 10 min with TBS buffer, and once for 10 min with PBS. Detection was performed using the enhanced chemiluminescence western blotting detection system (Millipore, MA, USA) (34).…”

Section: Methodsmentioning

confidence: 99%

Astragaloside II induces osteogenic activities of osteoblasts through the bone morphogenetic protein-2/MAPK and Smad1/5/8 pathways

et al. 2012

Self Cite

View full text Add to dashboard Cite

Abstract. Radix Astragalus has been identified to exert beneficial effects in preventing postmenopausal bone loss. However, the active ingredients and mechanism of action remain unknown. In this study, we examined the effect of Astragaloside II (AST II), which is a monomer of Astragalus saponin, on the viability, proliferation, differentiation and maturation of rat primary osteoblasts, as well as its relevant molecular mechanism. We found that AST II exhibits a significant induction of proliferation, differentiation and mineralization in primary osteoblasts. AST II stimulates osteoblast differentiation at various stages, from early to late stage of differentiated osteoblasts. Furthermore, induction of differentiation by AST II is associated with increased expression of bone morphogenetic protein-2 (BMP-2), activation of Smad1/5/8, ERK1/2 and p38, and increased expression of core-binding factor 1 (Cbfa1)/Runx2. BMP antagonist (Noggin) blocks the effect of AST II on cell differentiation, and Smad1/5/8, p38, Cbfa1 expression, but only partly decreases ERK1/2 activation. This indicates that BMP-2 is essential in AST II-mediated osteoblast differentiation and Smad1/5/8, p38, Cbfa1 activation, and is partly involved in ERK1/2 activation. In conclusion, although in vivo studies are required in the future, as a phyto-saponin of Radix Astragalus, AST II may become a novel candidate that is beneficial for stimulating the osteoblastic activity resulting in bone formation, which has not been recognized and reported previously.

show abstract

“…It has been demonstrated that the Chinese teasel root promotes the proliferation and differentiation of osteoblasts, and is able to regulate immune function, indicating that it may be used to treat arthritis (12). A kind of saponin in the medicinal herb Dipsacus asper wall has been used as an antiosteoporosis drug (13). However, its therapeutic mechanism of action remains unclear and further studies are required to screen and separate active components.…”

Section: Introductionmentioning

confidence: 99%

The molecular mechanism of treating osteoarthritis with dipsacus saponins by inhibiting chondrocyte apoptosis

Ren

et al. 2017

Exp Ther Med

View full text Add to dashboard Cite

Abstract. The present study aimed to determine the molecular mechanism of treating osteoarthritis with dipsacus saponins by inhibiting the apoptosis of chondrocytes. A total of 30 New Zealand rabbits were randomly divided into 2 groups: A control group and a model group. The osteoarthritis model was established using the HULTH method. The success of the model establishment was determined by pathological morphology and measurement of inflammatory cytokine levels. Chondrocytes were isolated and divided into 4 groups treated with varying concentrations of dipsacus saponins: 0, 25, 50 and l00 µg/l dipsacus saponins. Cell cycle distribution was analyzed using flow cytometry. Changes in cyclin D1, cyclin-dependent kinase 4 (CDK4) and p21 expression were detected by western blotting and changes in the levels of Bcl-2, Bax, caspase-3 and caspase-9 mRNA were detected using reverse transcription-quantitative polymerase chain reaction. The osteoarthritis model was established successfully, indicated by a significant increase in the levels of IL-1β, IL-6 and TNF-α in the model group (P<0.05) compared with the control group. The viability of the chondrocytes increased following treatment with dipsacus saponins in a concentration-dependent manner. The number of chondrocytes in the G0/G1 phase decreased in the 50 and l00 µg/l groups while the number of chondrocytes in the G2/M phase increased in the 50 and l00 µg/l groups. Levels of cyclin D1 and CDK4 expression increased following treatment with dipsacus saponins. Levels of Bax, caspase-3 and caspase-9 expression decreased while Bcl-2 levels increased following treatment with dipsacus saponins. The viability of chondrocytes increased following treatment with dipsacus saponins in a concentration-dependent manner. Thus, dipsacus saponins inhibited the apoptosis of chondrocytes by up-regulating Bcl-2 and down-regulating caspase-9, caspase-3 and Bax expression.

show abstract

Asperosaponin VI, A Saponin Component from Dipsacus asper Wall, induces Osteoblast Differentiation through Bone Morphogenetic Protein‐2/p38 and Extracellular Signal‐regulated Kinase 1/2 Pathway

Cited by 75 publications

References 29 publications

Potential Antiosteoporotic Agents from Plants: A Comprehensive Review

Potential Antiosteoporotic Agents from Plants: A Comprehensive Review

Astragaloside II induces osteogenic activities of osteoblasts through the bone morphogenetic protein-2/MAPK and Smad1/5/8 pathways

The molecular mechanism of treating osteoarthritis with dipsacus saponins by inhibiting chondrocyte apoptosis

Contact Info

Product

Resources

About