Aspergillus uvarum sp. nov., an uniseriate black Aspergillus species isolated from grapes in Europe

Perrone, Giancarlo; Varga, János; Susca, Antonia; Frisvad, Jens Christian; Stea, Gaetano; Kocsubé́, Sándor; Töth, Beáta; Kozakiewicz, Z.; Samson, Robert A.

doi:10.1099/ijs.0.65463-0

Cited by 81 publications

(60 citation statements)

References 28 publications

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“…These results are in agreement with previous findings reported by Perrone et al (2008) and Soni et al (2010) who found that filamentous fungi particularly Aspergillus and Trichoderma spp. are well known efficient producers of cellulases.…”

Section: Optimization Of Culture Conditions For Production Of Cellulasesupporting

confidence: 83%

culture conditions for production of cellulase by Aspergillus tubingensis KY615746 using rice straw waste

El-Nahrawy

Metwally

El-Kodoos

et al. 2017

EBSS

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TWENTY isolates of cellulase producing fungi were isolated from six samples of rotted rice straw which collected from different localities in El-Gharbia Governorate (Basyion), Kafrelsheikh Governorate (Sakha) and El-Dakahlia Governorate (El-Mansoura). On the basis of hydrolysis zone surrounding the colonies, an efficient fungus isolate (F7) for highest cellulolytic activity were chosen. According to morphological and biochemical characteristics as well as 18S rRNA sequence indicated that the isolate belonged to Aspergillus tubingensis KY615746. Effect of culture conditions for production of cellulase enzyme by A. tubingensis were investigated under Submerged Fermentation (SMF) and Solid State Fermentation (SSF) techniques. The optimum conditions for cellulase production by A. tubingensis were the best inoculum size was found to be 3%, pH 4, temperature 30 °C and incubation period 6th day where the reducing sugars attained (0.675, 0.728, 0.731 and 0.913 mg ml -1 ) with maximum activity (0.050, 0.055, 0.053 and 0.063 U ml -1 ), respectively for SMF conditions.On the other hand, the maximum cellulase activity under SSF technique with moisture level 10g : 30ml were (0.16 Ug -), incubation period 9 th day, inoculum size 4 ml:10g, pH 5 and temperature 30 °C where the reducing sugars were (1.412, 1.532, 1.551 and 1.521 mg g -1 ) with maximum activity (0.20, 0.22, 0.23 and 0.23 Ug -1 ), respectively. Based on the results, it may be concluded that rice straw waste can be a potential substrate for produce cellulase by SSF technique.

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Section: Optimization Of Culture Conditions For Production Of Cellulasesupporting

confidence: 83%

culture conditions for production of cellulase by Aspergillus tubingensis KY615746 using rice straw waste

El-Nahrawy

Metwally

El-Kodoos

et al. 2017

EBSS

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“…In general, black Aspergillus species can be distinguished via a polyphasic approach, using morphology, physiology and molecular data (Samson et al, 2007); nevertheless, most of the species belonging to Aspergillus section Nigri are usually considered as A. niger based on only morphological characters (Martínez-Culebras & Ramon, 2007 Recently, clinical isolates thought to belong to A. niger have been reclassified by genetic tools as A. tubingensis, A. awamori, A. uvarum and A. brasiliensis (Perrone et al, 2008;Manikandan et al, 2010;Hendrickx et al, 2012;Szigeti et al, 2012). Accordingly, A. niger is not the only black Aspergillus species involved in infections, Indeed A. tubingensis and other black Aspergillus species are also able to cause infections.…”

Section: Black Aspergillus Species Isolated From Clinical and Environmentioning

confidence: 99%

Black Aspergillus species isolated from clinical and environmental samples in Iran

Zarei

Mirhendi

Motamedi

et al. 2015

Journal of Medical Microbiology

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“…Recently, the Aspergillus ''uniseriate'' population from grapes in Europe was characterized molecularly as a population quite different from A. japonicus and A. aculeatus (Perrone et al 2006b). This population is being described as a new species to be called A. uvarum (Perrone et al 2007d).…”

Section: Introductionmentioning

confidence: 99%

Managing ochratoxin A risk in the grape-wine food chain

Visconti

Perrone

Cozzi

et al. 2008

Food Additives & Contaminants: Part A

121

102

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The main source of ochratoxin A (OTA) in the wine food chain is the infection of grapes by ''black aspergilli'' in the field. OTA-producing black aspergilli include principally Aspergillus carbonarius, followed by A. niger and possibly A. tubingensis. They are opportunistic fungi that develop particularly on damaged berries at ripening, although they may occur and form OTA on grapes from veraison to harvest. Climatic conditions (high humidity and temperature) and geographical location are important factors favouring OTA accumulation in grape berries. The severity of aspergillus rot is influenced by excessive irrigation and rainfall prior to harvest, which causes berry splitting. In addition, berry wounds caused by insect attack provide preferential entries for black aspergilli. High OTA levels occur in grapes severely damaged by the grape moth, Lobesia botrana, particularly in Mediterranean areas. Some grape varieties display greater susceptibility to aspergillus rot due to intrinsic genetic characteristics and bunch conformation (i.e. compact4sparse). Control measures for toxigenic mycoflora in the vineyards must consider these critical control points. Proper fungicidal and insecticidal treatments can reduce OTA contamination. Nevertheless, knowledge about the fate of OTA and its distribution in wine and winery byproducts is important to manage OTA risk in contaminated stock. In our wine-making experiments, only 4% of the OTA present in grapes remained in the wine -the majority is retained in pressed grape pomaces. OTA concentration remained unchanged in wine after a 1-year aging as well as in all liquid fractions collected during vinification (i.e. must, free run wine, and wine after first and second decantation). Activated carbon can reduce OTA levels in wine but negatively affects wine quality.

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Aspergillus uvarum sp. nov., an uniseriate black Aspergillus species isolated from grapes in Europe

Cited by 81 publications

References 28 publications

culture conditions for production of cellulase by Aspergillus tubingensis KY615746 using rice straw waste

culture conditions for production of cellulase by Aspergillus tubingensis KY615746 using rice straw waste

Black Aspergillus species isolated from clinical and environmental samples in Iran

Managing ochratoxin A risk in the grape-wine food chain

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