Aspergillus niger Culture Filtrate (ACF) Mediated Biocontrol of Enteric Pathogens in Wastewater

Subhash, Suja; Babu, Pradeesh; Vijayakumar, A.; Suresh, R.; Madhavan, Ajith; Nair, Bipin G.; Pal, Sanjay

doi:10.3390/w14010119

Cited by 6 publications

(4 citation statements)

References 58 publications

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“…In one of the few studies showing that A. niger has antimicrobial properties, Subhash et al (2022) evaluated the antibacterial activity of A. niger culture filtrate (ACF) against enteric pathogens Escherichia coli, Klebsiella pneumoniae, K. variicola, Pseudomonas aeruginosa, Salmonella enterica, Shigella dysenteriae, Staphylococcus aureus and Vibrio cholerae and demonstrated that ACF inhibited the growth of these pathogens (Subhash et al 2022). Similarly, in our study, A. niger culture filtrate was found to have antimicrobial activity against S. aureus and MRSA.…”

Section: Discussionmentioning

confidence: 99%

ISOLATION OF A NOVEL ANTIMICROBIAL POLYPEPTIDE FROM AN Aspergillus niger ISOLATE

ÜSTÜN,

YAZICI,

ÖRTUCU

2023

Trakya University Journal of Natural Sciences

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In this study the extracellular proteins from the isolate LC3 belonging to Aspergillus were purified for new antimicrobial polypeptide (AMP) discovery and then tested for antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Methicillin-resistant S. aureus (MRSA). Antimicrobial activity was determined by the trypsin/proteinase K assay, which was polypeptide-based, and it was observed that this protein was a protein of about 11 kDa by gel overlay assay. The minimum inhibitory concentration of purified AMP molecule against S. aureus ATCC 25923 and MRSA was 8 µg/ml and 32 µg/ml, respectively and the AMP molecule was confirmed. ITS sequence analysis showed that isolate LC3 was identified as Aspergillus niger, using the Bioedit sequence assembly program. The sequence was deposited with the GenBank database with accession number MK332597. The results indicate that the purified AMP molecule has the potential to be used in infections caused by S. aureus.

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Section: Discussionmentioning

confidence: 99%

ISOLATION OF A NOVEL ANTIMICROBIAL POLYPEPTIDE FROM AN Aspergillus niger ISOLATE

ÜSTÜN,

YAZICI,

ÖRTUCU

2023

Trakya University Journal of Natural Sciences

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“…The susceptibility of the strains (RS and T1) to antibiotics was evaluated by the antibiotic sensitivity test by conventional Kirby-Bauer disc diffusion assay [ 32 ] with 10 different antibiotics, amoxicillin, (10mcg) chloramphenicol (30mcg), penicillin G (2 U), gentamicin (10 mcg), cefoxitin (30mcg), streptomycin (10 mcg), ampicillin (10mcg), amikacin (30mcg), erythromycin (15mcg), tetracycline (30mcg) (HiMedia). Overnight cultures of strains with OD (600) of 1 were swabbed uniformly across MRS agar plates; the antibiotic discs were placed and incubated for 24 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…In the L4 stage, the worms were exposed to the following treatments (a) C. elegans + S. enterica (b) C. elegans + S. enterica + BLIS from RS (c) C. elegans + S. enterica + antibiotic (ciprofloxacin 20 μg/mL) (positive control) and (d) C. elegans + M9 buffer (control). The survival percentage of the worms treated with BLIS was compared with L4 worms in M9 broth, and the survival curves were plotted and compared ( 32 ).…”

Section: Methodsmentioning

confidence: 99%

Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy

Prakash,

Madhavan,

Veedu

et al. 2023

Front. Nutr.

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IntroductionEnvironmental enteropathy (EE), a chronic small intestine disease characterized by gut inflammation, is widely prevalent in low-income countries and is hypothesized to be caused by continuous exposure to fecal contamination. Targeted nutritional interventions using potential probiotic strains from fermented foods can be an effective strategy to inhibit enteric pathogens and prevent chronic gut inflammation.MethodsWe isolated potential strains from fermented rice water and lemon pickle and investigated their cell surface properties, antagonistic properties, adhesion to HT-29 cells, and inhibition of pathogen adherence to HT-29 cells. Bacteriocin-like inhibitory substances (BLIS) were purified, and in vivo, survival studies in Caenorhabditis elegans infected with Salmonella enterica MW116733 were performed. We further checked the expression pattern of pro and anti-inflammatory cytokines (IL-6, IL8, and IL-10) in HT-29 cells supplemented with strains.ResultsThe strains isolated from rice water (RS) and lemon pickle (T1) were identified as Limosilactobacillus fermentum MN410703 and MN410702, respectively. Strains showed probiotic properties like tolerance to low pH (pH 3.0), bile salts up to 0.5%, simulated gastric juice at low pH, and binding to extracellular matrix molecules. Auto-aggregation of T1 was in the range of 85% and significantly co-aggregated with Klebsiella pneumoniae, S. enterica, and Escherichia coli at 48, 79, and 65%, respectively. Both strains had a higher binding affinity to gelatin and heparin compared to Bacillus clausii. Susceptibility to most aminoglycoside, cephalosporin, and macrolide classes of antibiotics was also observed. RS showed BLIS activity against K. pneumoniae, S. aureus, and S. enterica at 60, 48, and 30%, respectively, and the protective effects of BLIS from RS in the C. elegans infection model demonstrated a 70% survival rate of the worms infected with S. enterica. RS and T1 demonstrated binding efficiency to HT-29 cell lines in the 38–46% range, and both strains inhibited the adhesion of E. coli MDR and S. enterica. Upregulation of IL-6 and IL-10 and the downregulation of IL-8 were observed when HT-29 cells were treated with RS, indicating the immunomodulatory effects of the strain.DiscussionThe potential strains identified could effectively inhibit enteric pathogens and prevent environmental enteropathy.

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“…The isolates of Bacillus spp. and Pseudomonas putida were isolated from soil, while Escherichia coli sequence type 155 (Salim et al 2019) and Klebsiella variicola (Subhash et al 2022) were isolated from sewage at the Sanitation Biotechnology Lab, School of Biotechnology, Amrita Vishwa Vidyapeetham, Kerala, India. All the bacterial strains were maintained in Luria-Bertani (LB) at 37 °C.…”

Section: Bacterial Culturesmentioning

confidence: 99%

A novel and improved selective media for the isolation and enumeration of Klebsiella species

Prasad

Shetty

Nair

et al. 2022

Appl Microbiol Biotechnol

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Bacterial pathogens are fostered in and transmitted through wastewater. Hence, monitoring their impact on sanitation and hygiene is imperative. As part of the monitoring process, culture-based methodologies are primarily used, which centre on the use of selective and differential media. Media available today are, at best, difficult to formulate and, at worst, prohibitively expensive. To address this lacuna, the study proposes a selective and differential medium for Klebsiella spp. Klebsiella blue agar (KBA) is completely selective against selected gram-positive bacteria (Bacillus spp., Staphylococcus aureus) and a few gram-negative bacteria (Acinetobacter baumanii, Serratia marcescens). On the other hand, it supports the growth of the chosen members of the Klebsiella pneumoniae species-complex with a characteristic green colouration. Methylene blue, tryptophan, and bile salt make up the selective components of KBA. Moreover, methylene blue, 0.6% NaCl, and glycerol render it differential. KBA was more selective than HiCrome™ Klebsiella Selective Agar Base (KSA) in replica plating experiments. KBA promoted only 157 CFUs against 209 CFUs in KSA when stamped with 253 CFUs grown on LB. The colonies so isolated were predominantly Klebsiella spp., on identification through colony polymerase chain reaction. Moreover, the differential nature of KBA distinguished Klebsiella aerogenes from other species. On the contrary, KSA lodged colonies indistinguishable from each other and Klebsiella spp. Due to its ease of formulation, high selectivity, differential nature, and cost-effective composition, KBA is a viable option for the routine culture of Klebsiella spp. in environmental and clinical settings. Key points • Formulated a novel selective and differential media for Klebsiella spp., named Klebsiella Blue agar • Facile formulation methodology • Can be employed to isolate Klebsiella spp. from complex sources such as wastewater

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Aspergillus niger Culture Filtrate (ACF) Mediated Biocontrol of Enteric Pathogens in Wastewater

Cited by 6 publications

References 58 publications

ISOLATION OF A NOVEL ANTIMICROBIAL POLYPEPTIDE FROM AN Aspergillus niger ISOLATE

ISOLATION OF A NOVEL ANTIMICROBIAL POLYPEPTIDE FROM AN Aspergillus niger ISOLATE

Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy

A novel and improved selective media for the isolation and enumeration of Klebsiella species

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