Aspergillus Metabolome Database for Mass Spectrometry Metabolomics

Gil-de-la-Fuente, Alberto; Mamani-Huanca, Maricruz; Stroe, Maria C.; Saugar, Sergio; García-Álvarez, Alejandra; Brakhage, Axel A.; Barbas, Coral; Otero, Abraham

doi:10.3390/jof7050387

Cited by 6 publications

(6 citation statements)

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“…Although metabolomics is an essential technology for the study of fungal secondary metabolites, identification and precise quantification of new fungal secondary metabolites remains a challenge 75 . To date, there is no database specific to phytotoxic fungal secondary metabolites, and the existing databases on natural products in general have limitations (Table 2) so that it is difficult to characterize the metabolites on the basis of chromatography and MS data 87 . The increasing use of molecular network tools (e.g., GNPS, 84,85 Metgem 86 ) and the coupling of metabolomics with other ‐omics technologies such as genomics make it possible to remedy this gap and to calmly envisage important progress in the knowledge for widespread use of phytotoxic fungal secondary metabolites as herbicides.…”

Section: Discussionmentioning

confidence: 99%

“…The greatest ensuing difficulty is the precise identification of the extracted metabolites, which often requires the mass/charge ratio (m/z) of the ions of interest. Although this ratio is provided by the MS system, to date there is no database allowing direct search of metabolites on the basis of their m/z ratio 87 . To overcome this shortcoming, specific databases, such as those corresponding to the secondary metabolites produced by Aspergillus sp ., have been created on the basis of the m/z ratio to meet specific needs 87 .…”

Section: Methodological Approaches Used To Discover Identify and Expl...mentioning

confidence: 99%

“…Although this ratio is provided by the MS system, to date there is no database allowing direct search of metabolites on the basis of their m/z ratio 87 . To overcome this shortcoming, specific databases, such as those corresponding to the secondary metabolites produced by Aspergillus sp ., have been created on the basis of the m/z ratio to meet specific needs 87 . With nearly 2811 compounds listed, this database is freely accessible online (https://github.com/albertogilf/ceuMassMediator/tree/master/CMMAspergillusDB) and is intended to be updated by future projects.…”

Section: Methodological Approaches Used To Discover Identify and Expl...mentioning

confidence: 99%

“…87 To overcome this shortcoming, specific databases, such as those corresponding to the secondary metabolites produced by Aspergillus sp., have been created on the basis of the m/z ratio to meet specific needs. 87 With nearly 2811 compounds listed, this database is freely accessible online (https://github.com/albertogilf/ ceuMassMediator/tree/master/CMMAspergillusDB) and is intended to be updated by future projects. Another interesting example is the older database based on MS analysis results (UV and positive and negative ESI).…”

Section: Identification Of Metabolitesmentioning

confidence: 99%

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Phytotoxic fungal secondary metabolites as herbicides

Bendejacq‐Seychelles,

Gibot‐Leclerc,

Guillemin

et al. 2023

Pest Management Science

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Among the alternatives to synthetic plant protection products, biocontrol appears as a promising method. This review reports on the diversity of fungal secondary metabolites phytotoxic to weeds and on the approach generally used to extract, characterize, identify and exploit them for weed management. The 183 phytotoxic fungal secondary metabolites discussed in this review fall into five main classes of molecules: 61 polyketides, 53 terpenoids, 36 nitrogenous metabolites, 18 phenols and phenolic acids, and 15 miscellaneous. They are mainly produced by the genera Drechslera, Fusarium and Alternaria. The phytotoxic effects, more often described by the symptoms they produce on plants than by their mode of action, range from inhibition of germination to inhibition of root and vegetative growth, including tissue and organ alterations. The biochemical characterization of fungal secondary metabolites requires expertise and tools to carry out fungal cultivation and metabolite extraction, phytotoxicity tests, purification and fractionation of the extracts, and chemical identification procedures. Phytotoxicity tests are mainly carried out under controlled laboratory conditions (not always on whole plants), while effectiveness against targeted weeds and environmental impacts must be assessed in greenhouses and open fields. These steps are necessary for the formulation of effective, environment‐friendly fungal secondary metabolites‐derived bioherbicides using new technologies such as nanomaterials.This article is protected by copyright. All rights reserved.

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Section: Discussionmentioning

confidence: 99%

Section: Methodological Approaches Used To Discover Identify and Expl...mentioning

confidence: 99%

Section: Methodological Approaches Used To Discover Identify and Expl...mentioning

confidence: 99%

Section: Identification Of Metabolitesmentioning

confidence: 99%

See 2 more Smart Citations

Phytotoxic fungal secondary metabolites as herbicides

Bendejacq‐Seychelles,

Gibot‐Leclerc,

Guillemin

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…Apart from macrolides, in either sample, 7α,27dihydroxycholesterol was identified, which belongs to the terpen(oid) class. It derives from cholesterol, and has been reported before in A. fumigatus metabolome (Gil-De-la-fuente et al, 2021). It is functionally relevant, helping the fungus to bypass the effects of ergosterol inhibitor class of antifungals (Xiong et al, 2005); a potential new drug target.…”

Section: Lc-ms/ms Analyses Of Aspergillus Fumigatus Extracts Derived ...mentioning

confidence: 93%

Untargeted Metabolomics Sheds Light on the Secondary Metabolism of Fungi Triggered by Choline-Based Ionic Liquids

et al. 2022

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Fungal secondary metabolites constitute a rich source of yet undiscovered bioactive compounds. Their production is often silent under standard laboratory conditions, but the production of some compounds can be triggered simply by altering the cultivation conditions. The usage of an organic salt – ionic liquid – as growth medium supplement can greatly impact the biosynthesis of secondary metabolites, leading to higher diversity of compounds accumulating extracellularly. This study examines if such supplements, specifically cholinium-based ionic liquids, can support the discovery of bioactive secondary metabolites across three model species: Neurospora crassa, Aspergillus nidulans, and Aspergillus fumigatus. Enriched organic extracts obtained from medium supernatant revealed high diversity in metabolites. The supplementation led apparently to increased levels of either 1-aminocyclopropane-1-carboxylate or α-aminoisobutyric acid. The extracts where bioactive against two major foodborne bacterial strains: Staphylococcus aureus and Escherichia coli. In particular, those retrieved from N. crassa cultures showed greater bactericidal potential compared to control extracts derived from non-supplemented cultures. An untargeted mass spectrometry analysis using the Global Natural Product Social Molecular Networking tool enabled to capture the chemical diversity driven by the ionic liquid stimuli. Diverse macrolides, among other compounds, were putatively associated with A. fumigatus; whereas an unexpected richness of cyclic (depsi)peptides with N. crassa. Further studies are required to understand if the identified peptides are the major players of the bioactivity of N. crassa extracts, and to decode their biosynthesis pathways as well.

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