Aspergillus gaarensis, a new addition to section Circumdati from soil of Lake El-Gaar in Wadi-El-Natron, Egypt

Al‐Bedak, Osama A.

doi:10.5943/sif/5/1/5

Cited by 18 publications

(16 citation statements)

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“…The universal primers ITS1 and ITS4 79 were used for amplification of the internal transcribed spacer (ITS) region, ACT783R and ACT512F for amplification of ACT gene 80 , and LROR and LR7 81 for amplification of the large subunit (LSU). PCR was done following Al-Bedak and Moubasher 82 .…”

Section: Methodsmentioning

confidence: 99%

Production of cold-active pectinases by three novel Cladosporium species isolated from Egypt and application of the most active enzyme

Moharram

Zohri

Hesham

et al. 2022

Sci Rep

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Cladosporium parasphaerospermum, Cladosporium chlamydosporigenum, and Cladosporium compactisporum have all been discovered and characterized as new Cladosporium species. The three new species seemed to generate cold-active pectinases with high activity at pH 6.0 and 10 °C, pH 6.0 and 15 °C, and pH 5.0 and 15 °C, respectively, with the most active being C. parasphaerospermum pectinase. In submerged fermentation (SmF), C. parasphaerospermum produced the most cold-active pectinase with the highest activity and specific activity (28.84 U/mL and 3797 U/mg) after 8 days. C. parasphaerospermum cold-active pectinase was isolated using DEAE-Cellulose anion exchange resin and a Sephadex G 100 gel filtration column. The enzyme was purified 214.4-fold and 406.4-fold greater than the fermentation medium using DEAE-cellulose and Sephadex G 100, respectively. At pH 7.0 and 10 °C, pure pectinase had the highest activity (6684 U/mg), with Km and Vmax determined to be 26.625 mg/mL and 312.5 U/min, respectively. At 5 mM/mL, EDTA, MgCl2, and SDS inhibited the activity of pure pectinase by 99.21, 96.03, and 94.45%, respectively. The addition of 10 U/mL pure pectinase enhanced the yield of apple, orange, apricot, and peach juice by 17, 20, 13, and 24%, respectively, and improved the clarity and colour of orange juice by 194 and 339%, respectively. We can now add cold-active pectinase production to the long list of Cladosporium species that have been identified. We also report three new species that can be used in biotechnological solutions as active microbial pectinase producers. Although further research is needed, these distinct species might be used to decompose difficult and resistant pertinacious wastes as well as clear fruit juices.

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Section: Methodsmentioning

confidence: 99%

Production of cold-active pectinases by three novel Cladosporium species isolated from Egypt and application of the most active enzyme

Moharram

Zohri

Hesham

et al. 2022

Sci Rep

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“…DNA extraction was performed as described by Moubasher et al [26]. The PCR reaction was carried out in SolGent company (South Korea) using the universal primers ITS1 (5TCC GTA GGT GAA CCT GCG G 3), and ITS4 (5TCC TCC GCT TAT TGA TAT GC 3), in the reaction mixture described by [27][28][29]. Sequences obtained from SolGent Company were compared to sequences from GenBank using MAFFT (version 6.861b) with the default options [30].…”

Section: Molecular Identification Of the Potent Strainsmentioning

confidence: 99%

Keratinases Produced by Aspergillus stelliformis, Aspergillus sydowii, and Fusarium brachygibbosum Isolated from Human Hair: Yield and Activity

Alwakeel

Ameen

Gwaiz

et al. 2021

JoF

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Twenty fungal strains belonging to 17 species and isolated from male scalp hair were tested for their capacity to hydrolyze keratinous material from chicken feather. The identification of the three most efficient species was confirmed by sequencing of the internal transcribed spacer (ITS) region of rDNA. Activities of fungal keratinases produced by Aspergillus stelliformis (strain AUMC 10920), A. sydowii (AUMC 10935), and Fusarium brachygibbosum (AUMC 10937) were 113, 120, and 130 IU mg−1 enzymes, respectively. The most favorable conditions were at pH 8.0 and 50 °C. Keratinase activity was markedly inhibited by EDTA and metal ions Ca+2, Co+2, Ni+2, Cu+2, Fe+2, Mg+2, and Zn+2, with differences between the fungal species. To the best of our knowledge, this is the first study on the activity of keratinase produced by A. stelliformis, A. sydowii, and F. brachygibbosum. F. brachygibbosum keratinase was the most active, but the species is not recommended because of its known phytopathogenicty. Aspergillus sydowii has many known biotechnological solutions and here we add another application of the species, as producer of keratinases. We introduce A. stelliformis as new producer of active fungal keratinases for biotechnological solutions, such as in the management of keratinous waste in poultry industry.

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“…The DNA extraction was performed following CTAB method described in Moubasher et al 24 The universal primers ITS1 and ITS4 25 were used for amplification of the internal transcribed spacer (ITS) region. 26,9…”

Section: Isolatesmentioning

confidence: 99%

Production of Cocktail Enzymes by Three Cladosporium Isolates and Bioconversion of Orange Peel Wastes into Valuable Enzymes

Moharram¹,

Zohri²,

Hesham³

et al. 2021

J. Pure Appl. Microbiol.

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The current research demonstrates the biotechnological economization of accumulated and inefficiently used agro-industrial orange peel wastes to generate amylase, endoglucanase, exoglucanase, pectinase, and xylanase, industrially essential enzymes with growing demands in enzyme markets, from three Cladosporium isolates. In submerged fermentation (SmF) at 10°C, the isolate AUMC 10865 produced the highest level of amylase (4164 IU/gram dry substrate). Endoglucanase, exoglucanase and xylanase had development peaks (923 IU/gds, 2280 IU/gds, and 1646 IU/gds, respectively in case of Cladosporium sp. AUMC 11366. Pectinase produced the most (7840 IU/gds) in the strain AUMC 11340. At 30°C, the strain AUMC 11340 secretes the most amylase (4120 IU/gds), endoglucanase (2700 IU/gds) and xylanase (3220 IU/gds). Exoglucanase development reached the peak (8750 IU/gds) in the isolate AUMC 10865. The overall production (5570 IU/gds) was instead enhanced by pectinase in the AUMC 11366 isolate. In solid-state fermentation (SSF) at 10°C, the isolate AUMC 10865 outperformed the other two isolates producing 640.0 IU/gds amylase, 763.3 IU/gds endoglucanase, 771.0 IU/gds exoglucanase, 1273.23 IU/gds pectinase and 1062.0 IU/gds xylanase, while the isolate AUMC 11366 produced the least amount of 399.7 IU/gds, 410.0 IU/gds, 413.3 IU/gds, 558.7 IU/gds, and 548.0 IU/gds, respectively. At 30°C, the isolate AUMC 11340 was superiorly producing higher levels of amylase (973.3 IU/gds), endoglucanase (746.0 IU/gds), exoglucanase (1052.0 IU/gds), pectinase (1685.3 IU/gds) and xylanase (1340.0 IU/gds), whereas isolate AUMC 10865 generated the least amounts of amylase (556.7 IU/gds) and exoglucanase (452.7 IU/gfs), and the isolate AUMC 11366 produced the least endoglucanase (256.3 IU/gds), pectinase (857.7 IU/gfs) and xylanase (436.3 IU/gds) amounts.

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Aspergillus gaarensis, a new addition to section Circumdati from soil of Lake El-Gaar in Wadi-El-Natron, Egypt

Cited by 18 publications

References 0 publications

Production of cold-active pectinases by three novel Cladosporium species isolated from Egypt and application of the most active enzyme

Production of cold-active pectinases by three novel Cladosporium species isolated from Egypt and application of the most active enzyme

Keratinases Produced by Aspergillus stelliformis, Aspergillus sydowii, and Fusarium brachygibbosum Isolated from Human Hair: Yield and Activity

Production of Cocktail Enzymes by Three Cladosporium Isolates and Bioconversion of Orange Peel Wastes into Valuable Enzymes

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