Aspergillus flavipes methionine γ-lyase-dextran conjugates with enhanced structural, proteolytic stability and anticancer efficiency

Elsayed, Abdelrahman H.; Hassan, Abdalla E. A.; Shindia, Ahmed A.; Mohamed, Shaimaa G. A.; Sitohy, Mahmoud

doi:10.1016/j.molcatb.2016.11.002

Cited by 31 publications

(38 citation statements)

References 45 publications

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“…So many natural compounds are reported to use as antibiotics, anticancer agents. Many endophytic fungi isolated from different medicinal plants are reported to be main source of many secondary metabolites and pharmaceutical compounds with biotechnological roles [ 3 , 11 , 12 , 13 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 32 , 34 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 78 , 79 , 97 , 99 , 100 , 101 , 102 , 103 , 104 ]. Fusarubin and anhydrofusarubin, isolated from the Cladosporium species an endophyte of Rauwolfia serpentina , displayed a promising antimicrobial and antitumor activities [ 105 ].…”

Section: Sources Of Taxol Productionmentioning

confidence: 99%

“…Traditionally approaches mainly implemented starting from isolation of endophytes, culturing on desired media, biological activity-guided assessment, extraction, purification, identification and chromatographic and spectroscopic characterization of the bioactive molecules [ 13 , 33 , 61 , 78 , 97 , 146 ]. Although, such techniques are powerful tools for the identification and characterization of new desired metabolites, however, the isolation and screening for the target fungal isolate is a very laborious process, time- consuming, with long-step processing, requiring a lot of facilities.…”

Section: Genome Miningmentioning

confidence: 99%

“…As well as, these conserved genes of characterized pathways were labeled and used as probes in Southern hybridization experiments [ 154 ]. The primers deduced based on the highly conserved motifs of these signature genes, were used for PCR screening approaches [ 3 , 21 , 24 , 27 , 28 , 61 , 78 , 97 , 99 , 103 ].…”

Section: Genome Miningmentioning

confidence: 99%

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Exploiting the Biosynthetic Potency of Taxol from Fungal Endophytes of Conifers Plants; Genome Mining and Metabolic Manipulation

et al. 2020

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Endophytic fungi have been considered as a repertoire for bioactive secondary metabolites with potential application in medicine, agriculture and food industry. The biosynthetic pathways by fungal endophytes raise the argument of acquisition of these machineries of such complex metabolites from the plant host. Diterpenoids “Taxol” is the most effective anticancer drug with highest annual sale, since its discovery in 1970 from the Pacific yew tree, Taxus brevifolia. However, the lower yield of Taxol from this natural source (bark of T. brevifolia), availability and vulnerability of this plant to unpredicted fluctuation with the ecological and environmental conditions are the challenges. Endophytic fungi from Taxus spp. opened a new avenue for industrial Taxol production due to their fast growth, cost effectiveness, independence on climatic changes, feasibility of genetic manipulation. However, the anticipation of endophytic fungi for industrial Taxol production has been challenged by the loss of its productivity, due to the metabolic reprograming of cells, downregulating the expression of its encoding genes with subculturing and storage. Thus, the objectives of this review were to (1) Nominate the endophytic fungal isolates with the Taxol producing potency from Taxaceae and Podocarpaceae; (2) Emphasize the different approaches such as molecular manipulation, cultural optimization, co-cultivation for enhancing the Taxol productivities; (3) Accentuate the genome mining of the rate-limiting enzymes for rapid screening the Taxol biosynthetic machinery; (4) Triggering the silenced rate-limiting genes and transcriptional factors to activates the biosynthetic gene cluster of Taxol.

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Section: Sources Of Taxol Productionmentioning

confidence: 99%

Section: Genome Miningmentioning

confidence: 99%

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Exploiting the Biosynthetic Potency of Taxol from Fungal Endophytes of Conifers Plants; Genome Mining and Metabolic Manipulation

et al. 2020

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“…# PG-PMT2962 315-10 kDa). The entire molecular mass of the purified enzyme was determined by the native-PAGE [ 41 , 42 , 43 , 44 , 45 , 46 , 47 ].…”

Section: Methodsmentioning

confidence: 99%

Biochemical Properties of Tyrosinase from Aspergillus terreus and Penicillium copticola; Undecanoic Acid from Aspergillus flavus, an Endophyte of Moringa oleifera, Is a Novel Potent Tyrosinase Inhibitor

et al. 2021

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Tyrosinase is a copper-containing monooxygenase catalyzing the O-hydroxylation of tyrosine to 3,4-dihydroxyphenylalanine then to dopaquinone that is profoundly involved in melanin synthesis in eukaryotes. Overactivation of tyrosinase is correlated with hyperpigmentation that is metabolically correlated with severe pathological disorders, so, inhibition of this enzyme is the most effective approach in controlling the overproduction of melanin and its hazardous effects. Thus, searching for a powerful, selective inhibitor of human tyrosinase to limit the hyper-synthesis of melanin is a challenge. Unlike the difficulty of overexpression of human tyrosinase, using fungal tyrosinase as a model enzyme to the human one to evaluate the mechanistics of enzyme inhibition in response to various compounds is the most feasible strategy. Thus, the purification of highly catalytic-efficient fungal tyrosinase, exploring a novel inhibitor, and evaluating the mechanistics of enzyme inhibition are the main objectives of this work. Aspergillus terreus and Penicillium copticola were reported as the most potential tyrosinase producers. The biochemical properties suggest that this enzyme displays a higher structural and catalytic proximity to human tyrosinase. Upon nutritional bioprocessing by Plackett–Burman design, the yield of tyrosinase was increased by about 7.5-folds, compared to the control. The purified tyrosinase was strongly inhibited by kojic acid and A. flavus DCM extracts with IC50 values of 15.1 and 12.6 µg/mL, respectively. From the spectroscopic analysis, the main anti-tyrosinase compounds of A. flavus extract was resolved, and verified as undecanoic acid. Further studies are ongoing to unravel the in vivo effect and cytotoxicity of this compound in fungi and human, that could be a novel drug to various diseases associated with hyperpigmentation by melanin.

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“…the reaction solution containing 3 ml of 10 mM phosphate buffer pH 7, 0.2 ml of 0.2 M H 2 O 2 , and 0.1ml of the enzyme extract incubated for 10 min and absorbance measured at 240 nm ( Abhishek et al., 2010 ; El-Sayed et al., 2016 ).…”

Section: Methodsmentioning

confidence: 99%

Bioremediation and tolerance of zinc ions using Fusarium solani

Sayed

El-Sayed

2020

Heliyon

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Evaluating the mechanism of tolerance and biotransformation Zn(II) ions by Fusarium solani based on the different physiological was the objective of this work. The physical properties of synthesized ZnONPs was determined by UV-spectroscopy, transmission electron microscope, and X-ray powder diffraction. The structural and anatomical changes of F. solani in response to Zn(II) was examined by TEM and SEM. From the HPLC profile, oxalic acid by F. solani was strongly increased by about 10.5 folds in response to 200 mg/l Zn(II) comparing to control cultures. The highest biosorption potential were reported at pH 4.0 (alkali-treated biomass) and 5.0 (native biomass), at 600 mg/l Zn(II) concentration, incubation temperature 30 °C, and contact time 40 min (alkali-treated biomass) and 6 h (native biomass). From the FT-IR spectroscopy, the main functional groups implemented on this remediation were C–S stretching, C=O C=N, C–H bending, C–N stretching and N–H bending. From the EDX spectra, fungal cellular sulfur and phosphorus compounds were the mainly compartments involved on ZN(II) binding.

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Aspergillus flavipes methionine γ-lyase-dextran conjugates with enhanced structural, proteolytic stability and anticancer efficiency

Cited by 31 publications

References 45 publications

Exploiting the Biosynthetic Potency of Taxol from Fungal Endophytes of Conifers Plants; Genome Mining and Metabolic Manipulation

Exploiting the Biosynthetic Potency of Taxol from Fungal Endophytes of Conifers Plants; Genome Mining and Metabolic Manipulation

Biochemical Properties of Tyrosinase from Aspergillus terreus and Penicillium copticola; Undecanoic Acid from Aspergillus flavus, an Endophyte of Moringa oleifera, Is a Novel Potent Tyrosinase Inhibitor

Bioremediation and tolerance of zinc ions using Fusarium solani

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