Aspen pectate lyase Ptxt PL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield

Biswal, Ajaya K.; Soeno, Kazuo; Gandla, Madhavi Latha; Immerzeel, Peter; Pattathil, Sivakumar; Lucenius, Jessica; Serimaa, Ritva; Hahn, Michael G.; Moritz, Thomas; Jönsson, Leif J.; Israelsson-Nordström, Maria; Mellerowicz, Ewa J.

doi:10.1186/1754-6834-7-11

Cited by 72 publications

(76 citation statements)

References 52 publications

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“…Expression of genes in this module peaked where the hierarchical clustering indicated the first transcriptome reprogramming event (i/ii), thus marking the cambium. NM15 (cell expansion; Figure 3) was enriched for the Pfam domain pectate lyase and included the alpha-expansin PtEXPA1, which has a demonstrated role in xylem cell expansion (Gray-Mitsumune et al, 2004, and the xylem-specific pectate lyase PtPL1-27 (Biswal et al, 2014). The expression of this module was specific to sample cluster ii, consistent with genes in this module functioning in xylem cell expansion.…”

Section: Coexpression Network Analysis Reveals a Continuum Of Transcrmentioning

confidence: 75%

AspWood: High-Spatial-Resolution Transcriptome Profiles Reveal Uncharacterized Modularity of Wood Formation in Populus tremula

et al. 2017

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Trees represent the largest terrestrial carbon sink and a renewable source of ligno-cellulose. There is significant scope for yield and quality improvement in these largely undomesticated species, and efforts to engineer elite varieties will benefit from improved understanding of the transcriptional network underlying cambial growth and wood formation. We generated highspatial-resolution RNA sequencing data spanning the secondary phloem, vascular cambium, and wood-forming tissues of Populus tremula. The transcriptome comprised 28,294 expressed, annotated genes, 78 novel protein-coding genes, and 567 putative long intergenic noncoding RNAs. Most paralogs originating from the Salicaceae whole-genome duplication had diverged expression, with the exception of those highly expressed during secondary cell wall deposition. Coexpression network analyses revealed that regulation of the transcriptome underlying cambial growth and wood formation comprises numerous modules forming a continuum of active processes across the tissues. A comparative analysis revealed that a majority of these modules are conserved in Picea abies. The high spatial resolution of our data enabled identification of novel roles for characterized genes involved in xylan and cellulose biosynthesis, regulators of xylem vessel and fiber differentiation and lignification. An associated web resource (AspWood, http://aspwood.popgenie.org) provides interactive tools for exploring the expression profiles and coexpression network.

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Section: Coexpression Network Analysis Reveals a Continuum Of Transcrmentioning

confidence: 75%

AspWood: High-Spatial-Resolution Transcriptome Profiles Reveal Uncharacterized Modularity of Wood Formation in Populus tremula

et al. 2017

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“…PLL genes have been shown to exhibit a broad range of functions in plants, which depend on the degradation of demethylesterified HG and alteration of cell wall composition and structure (Yadav et al, 2009;Biswal et al, 2014). In the process of fruit ripening, the cell wall needs to loosen the xyloglucan-cellulose network and pectin solubilization, this processes increasing the access of degradative enzymes to their substrates (Brummell, 2006).…”

Section: Possible Molecular Mechanism Of Del1 Responsible For the Mutmentioning

confidence: 99%

“…It has also been shown to be expressed in a wide range of tissues (Palusa et al, 2007;Sun and van Nocker, 2010). In addition, recent research has revealed that the overexpression of aspen PtxtPL1-27 can increase the solubility of wood matrix polysaccharides (Biswal et al, 2014). The modification of pectins may be important in the field of biotechnology for improving woody biomass (Biswal et al, 2014).…”

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confidence: 99%

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

et al. 2017

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To better understand the molecular mechanisms behind plant growth and leaf senescence in monocot plants, we identified a mutant exhibiting dwarfism and an early-senescence leaf phenotype, termed dwarf and early-senescence leaf1 (del1). Histological analysis showed that the abnormal growth was caused by a reduction in cell number. Further investigation revealed that the decline in cell number in del1 was affected by the cell cycle. Physiological analysis, transmission electron microscopy, and TUNEL assays showed that leaf senescence was triggered by the accumulation of reactive oxygen species. The DEL1 gene was cloned using a map-based approach. It was shown to encode a pectate lyase (PEL) precursor that contains a PelC domain. DEL1 contains all the conserved residues of PEL and has strong similarity with plant PelC. DEL1 is expressed in all tissues but predominantly in elongating tissues. Functional analysis revealed that mutation of DEL1 decreased the total PEL enzymatic activity, increased the degree of methylesterified homogalacturonan, and altered the cell wall composition and structure. In addition, transcriptome assay revealed that a set of cell wall function-and senescence-related gene expression was altered in del1 plants. Our research indicates that DEL1 is involved in both the maintenance of normal cell division and the induction of leaf senescence. These findings reveal a new molecular mechanism for plant growth and leaf senescence mediated by PECTATE LYASE-LIKE genes.

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“…In addition, it was reported that association of microwave with chemical pretreatments are more effective than the formal heating chemical pretreatments [157]. Wheat bran pretreatment by using hydrothermal microwave and their results showed that hydrothermal microwave could increase biomass hydrolysis by 91% with high arabinoxylans [158].…”

Section: Physical Pretreatmentmentioning

confidence: 99%

Advances in Genetic Manipulation of Lignocellulose to Reduce Biomass Recalcitrance and Enhance Biofuel Production in Bioenergy Crops

Madadi¹,

Penga²,

Abbas³

2017

J Plant Biochem Physiol

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Lignocellulose biomass derived from plant cell walls is a rich source of biopolymers for the production of biofuels. Biomass recalcitrance is the noticeable and main features of lignocellulose which can reduces by genetic modification of plant cell wall. The aim of the present review is to provide the reader a new insight for enhancing biomass yield and biofuels production. This can be issued by focusing on major perennial grasses, cereal crops and woody feedstock which have high biomass yield or large biomass residues and also the effects of distinctive cell wall polymers (cellulose, hemicellulose, lignin, and pectin) on the enzymatic saccharification of biomass under different pretreatments. Moreover the present review paper will also major gene candidates which are involved plant cell wall biosynthesis, degradation and modification for improving biomass yield and digestibility in transgenic plants and genetic mutants.

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Aspen pectate lyase Ptxt PL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield

Cited by 72 publications

References 52 publications

AspWood: High-Spatial-Resolution Transcriptome Profiles Reveal Uncharacterized Modularity of Wood Formation in Populus tremula

AspWood: High-Spatial-Resolution Transcriptome Profiles Reveal Uncharacterized Modularity of Wood Formation in Populus tremula

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

Advances in Genetic Manipulation of Lignocellulose to Reduce Biomass Recalcitrance and Enhance Biofuel Production in Bioenergy Crops

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