Aspects of the Regulation of Adenylate Cyclase Synthesis in Escherichia coli K12

Roy, A. K.; Glaser, Philippe; Danchin, Antoine

doi:10.1099/00221287-134-2-359

Cited by 17 publications

(14 citation statements)

References 36 publications

(17 reference statements)

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“…8e). The cAMP pattern of the MC4100rpoS mutant becomes more like that in TP2100, another strain used as wild-type in earlier studies (Roy et al, 1988). Testing other phenotypes (response to H 2 0 2 and glycogen production) suggested that strain TP2100 is indeed an rpoS mutant.…”

Section: A Second Transition In Camp Levels At Very Low Glucose Concementioning

confidence: 85%

The relationship between external glucose concentration and cAMP levels inside Escherichia coli: implications for models of phosphotransferase-mediated regulation of adenylate cyclase

1997

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The concentration of glucose in the medium influences the regulation of CAMP levels in Escherichia coli. Growth in minimal medium with micromolar glucose results in 8-to 10-fold higher intracellular cAMP concentrations than observed during growth with excess glucose. Current models would suggest that the difference in cAMP levels between glucose-rich and glucose-limited states is due to altered transport flux through the phosphoenolpyruvate :glucose phosphotransferase system (PTS), which in turn controls adenylate cyclase. A consequence of this model is that cAMP levels should be inversely related to the saturation of the PTS transporter. To test this hypothesis, the relationship between external glucose concentration and CAMP levels inside E. coli were investigated in detail, both through direct CAMP assay and indirectly through measurement of expression of CAMP-regulated genes. Responses were followed in batch, dialysis and glucose-limited continuous culture. A sharp rise in intracellular cAMP occurred when the nutrient concentration in minimal medium dropped to approximately 0 3 mM glucose. Likewise, addition of >03 mM glucose, but not c03 mM glucose, sharply reduced the intracellular CAMP level of starving bacteria. There was no striking shift in growth rate or ['4C]glucose assimilation in bacteria passing through the 0 5 to 0.3 mM concentration threshold influencing CAMP levels, suggesting that neither metabolic flux nor transporter saturation influenced the sensing of nutrient levels. The (IIA/IIBC)G'C PTS is 96-97% saturated a t 0.3 mM glucose so these results are not easily reconcilable with current models of cAMP regulation. Aside from the transition in CAMP levels initiated above 0 3 mM, a second shift occurred below 1 pM glucose. Approaching starvation, well below saturation of the PTS, CAMP levels either increased or decreased depending on unknown factors that differ between common E. coli K-12 strains.

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Section: A Second Transition In Camp Levels At Very Low Glucose Concementioning

confidence: 85%

The relationship between external glucose concentration and cAMP levels inside Escherichia coli: implications for models of phosphotransferase-mediated regulation of adenylate cyclase

1997

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“…Addition of M9 to TCM strongly suppressed the inducing activity. To identify the components that were responsible for this suppression, single components of the minimal media M9 and MM1 (Roy et al, 1988), described for growth of X. campestris (de CrecyLegard et al, 1990), were added to TCM. TCM was used in a 10-fold dilution in water, still giving 75% of its original activity.…”

Section: Searchmentioning

confidence: 99%

A Xanthomonas Pathogenicity Locus Is Induced by Sucrose and Sulfur-Containing Amino Acids.

Schulte

Bonas

1992

Plant Cell

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Expression of hrp (hypersensitive reaction and pathogenicity) genes from Xanthomonas campestris pv vesicatoria is suppressed in complex media but induced in the plant. We examined the effects of macronutrients on transcription of hrp-gusA (P-glucuronldase) fusions by growth of the bacteria in defined medium. Modified MM1 minimal medium, supplemented with casamino acids, was able to induce hrpf strongly when sucrose or fructose was added as a carbon source. However, high concentrations of casamlno acids suppressed hrpF induction. Sulfur-containing amino acids were required for induction, with methionine induction being comparable to induction in plants. Both sucrose and methionine were required for induction. lnduction in medium optimal for hrpF induction, designated XVM1, occurred at pH 5.5 to pH 7.5. High concentrations of phosphate or sodium chlorlde suppressed gene activation. Gene induction was inhibited by succinate, citrate, pyruvate, and glutamine. Expression levels of different hrp loci from X. c. vesicatoria in XVMl varied, dependent on the genetic background of the Xanthomonas strain used. The results suggest that severa1 control mechanisms might be involved in the expression of hrp genes.

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“…Ap, ampicillin; Cm, chloramphenicol; Km, kanamycin; Tc, tetracycline; R , resistance; Pp, Pseudomonas putida ; Δ, deletion; :s, novel joint; lac Δ X74 , complete deletion of the lac operon; glp * is mutation near the argH gene that allows growth of this strain on glycerol [57].…”

Section: Methodsmentioning

confidence: 99%

CRP-Cyclic AMP Dependent Inhibition of the Xylene-Responsive σ54-Promoter Pu in Escherichia coli

et al. 2014

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The expression of σ54-dependent Pseudomonas putida Pu promoter is activated by XylR activator when cells are exposed to a variety of aromatic inducers. In this study, the transcriptional activation of the P. putida Pu promoter was recreated in the heterologous host Escherichia coli. Here we show that the cAMP receptor protein (CRP), a well-known carbon utilization regulator, had an inhibitory effect on the expression of Pu promoter in a cAMP-dependent manner. The inhibitory effect was not activator specific. In vivo KMnO4 and DMS footprinting analysis indicated that CRP-cAMP poised the RNA polymerase at Pu promoter, inhibiting the isomerization step of the transcription initiation even in the presence of an activator. Therefore, the presence of PTS-sugar, which eliminates cAMP, could activate the poised RNA polymerase at Pu promoter to transcribe. Moreover, the activation region 1 (AR1) of CRP, which interacts directly with the αCTD (C-terminal domain of α-subunit) of RNA polymerase, was found essential for the CRP-mediated inhibition at Pu promoter. A model for the above observations is discussed.

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Aspects of the Regulation of Adenylate Cyclase Synthesis in Escherichia coli K12

Cited by 17 publications

References 36 publications

The relationship between external glucose concentration and cAMP levels inside Escherichia coli: implications for models of phosphotransferase-mediated regulation of adenylate cyclase

The relationship between external glucose concentration and cAMP levels inside Escherichia coli: implications for models of phosphotransferase-mediated regulation of adenylate cyclase

A Xanthomonas Pathogenicity Locus Is Induced by Sucrose and Sulfur-Containing Amino Acids.

CRP-Cyclic AMP Dependent Inhibition of the Xylene-Responsive σ54-Promoter Pu in Escherichia coli

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