Aspects of rumen microbiology central to mechanistic modelling of methane production in cattle

Ellis, J.L.; Dijkstra, J.; Kebreab, E.; Bannink, A.; Odongo, N. E.; McBride, B.W.; France, J.

doi:10.1017/s0021859608007752

Cited by 218 publications

(216 citation statements)

References 138 publications

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“…The negative correlation between CP and methane production is in agreement with the finding of Moss (2000). Moreover, negative correlations of methane production with energy and ether extract are in agreement with previous finding by Yan et al(2009) and Ellis et al(2008), respectively.…”

Section: Association Between Methane Ivomd Vfa and Chemical Composisupporting

confidence: 92%

In vitro fermentation, digestibility and methane production of tropical perennial grass species

Gemeda

Hassen

2014

Crop Pasture Sci.

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Abstract. This study characterised 16 tropical perennial grass species in terms of in vitro methane output and related their digestibility and rumen fermentation with methane output. The grass samples were collected, dried in a forced oven, and ground and analysed for nutrient composition. In vitro gas production and organic matter digestibility (IVOMD) were determined using rumen fluid collected, strained and anaerobically prepared. A semi-automated system was used to measure gas production through in vitro incubation at 398C. Anthephora argentea and Stipagrostis ciliate produced the highest concentration of methane in terms of g kg -1 digestible dry matter (DDM) and g kg -1 digestible organic matter (IVOMD). Cenchrus ciliaris, Setaria verticillata and Panicum coloratum produced the lowest (P < 0.05) methane when expressed in terms of g kg -1 DDM and g kg -1 IVOMD. Ash, ether extract, non-fibrous carbohydrate, neutral and acid detergent insoluble nitrogen, and crude protein were negatively correlated with methane production. Methane production positively correlated with neutral and acid detergent fibre, cellulose and hemicellulose. It is important to focus on screening and selecting perennial grass with higher nitrogen content and low methane production to mitigate methane production under tropical conditions.

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Section: Association Between Methane Ivomd Vfa and Chemical Composisupporting

confidence: 92%

In vitro fermentation, digestibility and methane production of tropical perennial grass species

Gemeda

Hassen

2014

Crop Pasture Sci.

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“…This is involved in the conversion of carbon compounds in fatty acids (Ellis et al, 2008;Servinsky et al, 2012), and their higher diversity suggests Prevotella has a greater repertoire of enzymes involved in this process and supports previous reports that Prevotella species are among those (including Clostridium species) that play a role in biohydrogenation of dietary polyunsaturated fatty acids to saturated fatty acids (Huws et al, 2011). This would suggest that fatty acids may be a resource for which these organisms compete and for which they have diversified niches.…”

Section: Metabolic Adaptations In Prevotellasupporting

confidence: 85%

Erratum: Divergent functional isoforms drive niche specialisation for nutrient acquisition and use in rumen microbiome

Rubino¹,

Carberry²,

Waters³

et al. 2017

The ISME Journal

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Many microbes in complex competitive environments share genes for acquiring and utilising nutrients, questioning whether niche specialisation exists and if so, how it is maintained. We investigated the genomic signatures of niche specialisation in the rumen microbiome, a highly competitive, anaerobic environment, with limited nutrient availability determined by the biomass consumed by the host. We generated individual metagenomic libraries from 14 cows fed an ad libitum diet of grass silage and calculated functional isoform diversity for each microbial gene identified. The animal replicates were used to calculate confidence intervals to test for differences in diversity of functional isoforms between microbes that may drive niche specialisation. We identified 153 genes with significant differences in functional isoform diversity between the two most abundant bacterial genera in the rumen (Prevotella and Clostridium). We found Prevotella possesses a more diverse range of isoforms capable of degrading hemicellulose, whereas Clostridium for cellulose. Furthermore, significant differences were observed in key metabolic processes indicating that isoform diversity plays an important role in maintaining their niche specialisation. The methods presented represent a novel approach for untangling complex interactions between microorganisms in natural environments and have resulted in an expanded catalogue of gene targets central to rumen cellulosic biomass degradation.

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“…La mayor proporción de butirato indica que la concentración de H 2 se redujo, ya que durante la formación de este compuesto a partir de 1 mol de glucosa, se liberan menos H 2 comparados con la formación de acetato (Ungerfeld, 2015;Buccioni et al, 2015). De esta manera, los H 2 disponibles fueron usados en la producción de valerato (AGV glucogénico) Ellis et al, 2008) y para una mayor síntesis de biomasa microbiana (Leng y Preston, 2010).…”

Section: Discussionunclassified

Evaluación de diferentes niveles de inclusión de plantas nativas de sabanas inundables sobre una dieta basal de Brachiaria humidicola y su efecto sobre la producción de metano in vitro

et al. 2017

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Evaluación de diferentes niveles de inclusión de plantas nativas de sabanas inundables sobre una dieta basal de Brachiaria humidicola y su efecto sobre la producción de metano in vitro resUMen Se evaluó el efecto sobre la producción de metano de la inclusión creciente (25, 50 y 75 %) de cinco plantas nativas de sabanas inundables (Senna occidentalis, Enterolobium schomburgkii, Galactia jussiaeana, Belencita nemorosa y Ambrosia peruviana), sobre una dieta base de Brachiaria humidicola. Los tratamientos fueron incubados anaeróbicamente con fluido ruminal a 39°C por 24 h y después del proceso de fermentación, se determinó la producción de gas, metano, ácidos grasos volátiles (AGV), amonio y degradación de la materia seca (MSD). La inclusión en la dieta de 50 y 75% de todas las plantas, incrementaron el contenido de amonio ruminal, con valores entre 189 -282 mg/L. Ninguna de las plantas modificó la concentración de AGVT (44-62 mmol/L). La producción de metano no se redujo significativamente con ningún tratamiento (p>0,05), aunque con S. occidentalis (75%) este parámetro fue inferior en un 18%, además, se incrementó la MSD y los niveles de butirato, isobutirato y valerato. Con E. schomburgkii (75%), la metanogénesis también se redujo en un 15%, sin embargo, este efecto estuvo acompañado de una disminución en la MSD y producción de gas. En conclusión, ninguna de las plantas mostró potencial para reducir la producción de metano, no obstante, las especies se convierten en alternativas nutricionales útiles para complementar el aporte proteico de la dieta en los sistemas ganaderos ubicados en condiciones de sabanas inundables. The effect on methane production of increasing levels (25, 50 and 75%) of five native plants from the floodable savannas (Senna occidentalis, Enterolobium schomburgkii, Galactia jussiaeana, Belencita nemorosa y Ambrosia peruviana), on a Brachiaria humidicola based diet were evaluated. The treatments were anaerobically incubated with ruminal fluid at 39 °C for 24 h, after the incubation period, gas production, methane, volatile fatty acids (VFA), ammonia and dry matter degradation (DMD) were determined. The inclusion of 50 and 75 % of all plants in the diet, increased the ruminal ammonia content, with values between 189-282 mg/L. None of the plants modified TVFA concentration (44-62 mmol / L). Methane production was not significantly reduced with any treatment (p>0.05), although with Senna occidentalis (75%) this parameter was an 18% lower, in addition, DMD and butyrate, isobutyrate and valerate levels were increased. With Enterolobium schomburgkii (75%), methanogenesis was also reduced by 15%, however, this effect was accompanied by a decrease in DMD and gas production. In conclusion, none of the plants showed potential to reduce methane production, nevertheless, they become useful nutritional alternatives to supplement the dietary protein in the livestock systems located under floodable savanna conditions. additional keYwordsIn vitro fermentation. Antimethanogenic plants. Dose. Ruminants.Arch. Zoot...

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Aspects of rumen microbiology central to mechanistic modelling of methane production in cattle

Cited by 218 publications

References 138 publications

In vitro fermentation, digestibility and methane production of tropical perennial grass species

In vitro fermentation, digestibility and methane production of tropical perennial grass species

Erratum: Divergent functional isoforms drive niche specialisation for nutrient acquisition and use in rumen microbiome

Evaluación de diferentes niveles de inclusión de plantas nativas de sabanas inundables sobre una dieta basal de Brachiaria humidicola y su efecto sobre la producción de metano in vitro

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