Aspects of quantitative determinations with polarimetric detectors in liquid chromatography

Enhanced Polarimetric Detection in HPLC Using a Refractive Index Equalizer

“…where R(a) is the rotation matrix, T permits derivation of the expression of the output polarization vector Eout, which reads Emit ~TEir (tx cos a cos 0 -Ty sin a sin 0 Tx sin a cos 0 + tv cos a sin 0 (7) Using eq 7, it can be shown that Eout corresponds to a linear state of polarization, and that for Tx-ry = At « r" its azimuth j8 is given by THEORY Pseudorotation. The changes in the polarization properties of a light beam passing through a U-shaped flow cell filled with an inhomogenous medium is described using a simple model.…”

Chemical species

“…Polarimetric detectors continued to be exploited for the analysis of chiral compounds. Present detectors demonstrate relative standard deviations as low as 0.25% under static conditions, but under dynamic chromatographic conditions refractive index related effects can interfere with quantitative determinations (Dappen et al, 1993). An LC method for determination of the absolute configuration of alpha-chiral amines has been demonstrated using the chiral derivatizing agent N-succinimidyl exmethoxyphenylacetate.…”

Optical rotation detection for reversed phase HPLC: Investigation of solvent effects

“…This data suggests an improvement of up to 30% in sensitivity has been obtained by increasing the water content of the mobile phase. The occurrence of pseudorotation effects 8 will also have an impact on the observed sensitivity of a given mobile phase system and these effects have not been considered in the data analysis performed. …”

“…of a sample against an external calibration. [6][7][8] This approach removes the need to develop specific chiral separations on expensive columns.…”