Aspects of nitrogen dioxide toxicity in environmental urban concentrations in human nasal epithelium

Koehler, Christian; Ginzkey, Christian; Friehs, Gudrun; Hackenberg, Stephan; Froelich, Katrin; Scherzed, Agmal; Burghartz, Marc; Kessler, M.; Kleinsasser, N.

doi:10.1016/j.taap.2010.03.003

Cited by 47 publications

(27 citation statements)

References 34 publications

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“…Isolation and cultivation of the cells were carried out as previously described [5]. In brief, after isolation the cells of each donor were cultured on 27 porous membrane inserts (Corning® Transwell polycarbonate membrane inserts, 0.4 µm; 12 mm diameter; Corning Inc., New York, N.Y., USA), covered with 150 µl of collagen I (66 ng/ml; Sigma-Aldrich, St. Louis, Mo., USA).…”

Section: Methodsmentioning

confidence: 99%

“…For NO 2 exposure, a gas mixture construction was developed on the basis of mass-flow controllers (Bronkhorst High-Tech B.V., Ruurlo, The Netherlands), thus achieving a defined and controlled concentration of NO 2 by diluting NO 2 with synthetic air [5]. The mixture of 0.1 ppm NO 2 was made by mixing 10 ml/min of 10 ppm NO 2 with 990 ml/min of synthetic air (Linde Gas, Pullach, Germany) to achieve 1,000 ml/min of 0.1 ppm NO 2 .…”

Section: Methodsmentioning

confidence: 99%

“…Genotoxic effects of low NO 2 concentrations on nasal epithelial cells were previously analyzed by our group [5,6,7]. To summarize that work, the genotoxicity of NO 2 could be demonstrated at a concentration as low as 0.01 ppm NO 2 indicated by DNA fragmentation in the Comet assay and micronucleus formation.…”

Section: Introductionmentioning

confidence: 98%

“…Tunnicliffe et al [13] described a ‘no-effect concentration' of 0.1 ppm NO 2 , and Berglund et al [14] suggested a possible threshold at this concentration. On the basis of these experimental studies, the WHO recommended a year-limit value of 0.02 ppm NO 2 and a 1-hour-limit value of 0.1 ppm NO 2 [5,16]. …”

Section: Introductionmentioning

confidence: 99%

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The Proinflammatory Potential of Nitrogen Dioxide and Its Influence on the House Dust Mite Allergen Der p 1

Koehler

Paulus

Ginzkey

et al. 2016

Int Arch Allergy Immunol

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Asthma and allergies are both major global health problems with an increasing prevalence, and environmental data implicate an influence of air pollutants on their development. The present study focuses on the influence of nitrogen dioxide (NO₂) and the major allergen of the house dust mite Der p 1 on human nasal epithelial cells of nonallergic patients in vitro. Nasal epithelial mucosa samples of 11 donors were harvested during nasal air passage surgery and cultured as an air-liquid interface. Exposure to 0.1, 1 and 10 ppm NO₂ or synthetic air as a control was performed for 1 h. Subsequently, the cells were exposed to Der p 1 for 24 h. The release of interleukin (IL)-6 and IL-8 was measured by ELISA, and the production of IL-6 mRNA and IL-8 mRNA was measured by RT-PCR. NO₂ exposure resulted in a concentration-dependent release of IL-6, but not IL-8 release. The coexposure of 0.1 ppm NO₂ and Der p 1, or 1 ppm NO₂ and Der p 1 significantly increased both IL-6 and IL-8 release. Exposure to NO₂, Der p 1, or their combination, did not significantly influence the production of IL-6 or IL-8 mRNA. In conclusion, NO₂ increases the release of inflammatory cytokines in human nasal epithelial cells, especially in coexposure with Der p 1, as a mechanism of allergotoxicology.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Proinflammatory Potential of Nitrogen Dioxide and Its Influence on the House Dust Mite Allergen Der p 1

Koehler

Paulus

Ginzkey

et al. 2016

Int Arch Allergy Immunol

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show abstract

“…The resultant nasal wash fluid was taken to the Medical Biology Laboratory on the same day. Absence or presence of genotoxic damage to cells (15,16), obtained from nasal wash fluid from both groups, was determined using CA (17-21) by a researcher who was not involved in sample procurement.…”

Section: Patientsmentioning

confidence: 99%

E ect of mometasone furoate nasal spray on the DNA of nasal mucosal cells

2018

Turk J Med Sci

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Integrative analysis of mRNA and microRNA expression of a human alveolar epithelial cell(A549) exposed to water and organic‐soluble extract from particulate matter (PM)_2.5

Jeong

Song

Cho

et al. 2016

Environmental Toxicology

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MicroRNA (miRNA) is now attracting attention as a powerful negative regulator of messenger RNA(mRNA) levels, and is implicated in the modulation of important mRNA networks involved in toxicity. In this study, we assessed the effects of particulate matter 2.5 (PM ), one of the most significant air pollutants, on miRNA and target gene expression. We exposed human alveolar epithelial cell (A549) to two types of PM [water (W-PM ) and organic (O-PM ) soluble extracts] and performed miRNA microarray analysis. A total of 37 miRNAs and 62 miRNAs were altered 1.3-fold in W-PM and O-PM , respectively. Integrated analyses of miRNA and mRNA expression profiles identified negative correlations between miRNA and mRNA in both W-PM and O-PM exposure groups. Gene ontology and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses showed that the 35 W-PM target genes are involved in responses to nutrients, positive regulation of biosynthetic processes, positive regulation of nucleobase, nucleoside, and nucleotide, and nucleic acid metabolic processes; while the 69 O-PM target genes are involved in DNA replication, cell cycle processes, the M phase, and the cell cycle check point. We suggest that these target genes may play important roles in PM -induced respiratory toxicity by miRNA regulation. These results demonstrate an integrated miRNA-mRNA approach for identifying molecular events induced by environmental pollutants in an in vitro human model. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 302-310, 2017.

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Aspects of nitrogen dioxide toxicity in environmental urban concentrations in human nasal epithelium

Cited by 47 publications

References 34 publications

The Proinflammatory Potential of Nitrogen Dioxide and Its Influence on the House Dust Mite Allergen Der p 1

The Proinflammatory Potential of Nitrogen Dioxide and Its Influence on the House Dust Mite Allergen Der p 1

E ect of mometasone furoate nasal spray on the DNA of nasal mucosal cells

Integrative analysis of mRNA and microRNA expression of a human alveolar epithelial cell(A549) exposed to water and organic‐soluble extract from particulate matter (PM)_2.5

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Aspects of nitrogen dioxide toxicity in environmental urban concentrations in human nasal epithelium

Cited by 47 publications

References 34 publications

The Proinflammatory Potential of Nitrogen Dioxide and Its Influence on the House Dust Mite Allergen Der p 1

The Proinflammatory Potential of Nitrogen Dioxide and Its Influence on the House Dust Mite Allergen Der p 1

E ect of mometasone furoate nasal spray on the DNA of nasal mucosal cells

Integrative analysis of mRNA and microRNA expression of a human alveolar epithelial cell(A549) exposed to water and organic‐soluble extract from particulate matter (PM)2.5

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Product

Resources

About

Integrative analysis of mRNA and microRNA expression of a human alveolar epithelial cell(A549) exposed to water and organic‐soluble extract from particulate matter (PM)_2.5