Aspects of energetic substrate metabolism of in vitro and in vivo bovine embryos

Souza, Danielle Kaiser de; Salles, Loise Pedrosa; Silva, A.A.M. Rosa e

doi:10.1590/1414-431x20143744

Cited by 18 publications

(14 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whilst several differences between the two groups were observed, when both pregnancy and day effects were considered, only differences in threonine, glutamate, and valine flux in ULF were observed, thus offering scope for targeted experimentation into the role of these amino acids in day 7-19 embryo development. Moreover, the total amino acid content of ULF increased as the blastocyst progressed to an elongated filamentous conceptus, most likely to accommodate for the greater metabolic demands of the developing offspring (Souza et al, 2015). Interestingly, this data loosely corroborates earlier findings that total ULF amino acid concentrations are lower in sub-fertile animals (Meier et al, 2014) and in cattle carrying developmentally compromised (cloned) embryos (Groebner et al, 2011).…”

Section: Secretome Metabolomicssupporting

confidence: 86%

Understanding the uterine environment in early pregnancy in cattle: How have the omics en-hanced our knowledge?

Simintiras¹,

Forde²

2017

Anim Reprod

View full text Add to dashboard Cite

Early pregnancy loss in cattle can be attributed to a myriad of sources. One key factor that can influence early pregnancy success or loss is the influence and interactions between the maternal environment and the developing embryo/conceptus. Recent advances in high-throughput 'omics' technologies coupled with improved bioinformatics capabilities represent a promising avenue for enhancing our understanding of fundamental developmental events -which would have direct agricultural, veterinary, and economic benefits. Thusly this review revolves around recent applications of advanced transcriptomic, proteomic, and metabolomic analyses within a bovine uterine secretomic and interactomic context, with an overriding aim to highlight the advantages of these emerging fields whilst identifying areas for improvement, consideration, and further research and development.

show abstract

Section: Secretome Metabolomicssupporting

confidence: 86%

Understanding the uterine environment in early pregnancy in cattle: How have the omics en-hanced our knowledge?

Simintiras¹,

Forde²

2017

Anim Reprod

View full text Add to dashboard Cite

show abstract

“…Tead4 is involved in preventing excess accumulation of ROS in murine embryos [8]. In bovine embryos, the similar patterns of glucose and O 2 consumption have been reported [25,26,27]. In general, 5% O 2 is adopted as a standard culture condition for bovine embryos to protect them from oxidative stress [28].…”

Section: Discussionmentioning

confidence: 92%

Effects of downregulating TEAD4 transcripts by RNA interference on early development of bovine embryos

Sakurai

Takahashi

Emura

et al. 2017

Journal of Reproduction and Development

View full text Add to dashboard Cite

Transcription factor TEA domain family transcription factor 4 (Tead4) is one of the key factors involved in the differentiation of the trophectoderm (TE) in murine embryos. However, knowledge on the roles of TEAD4 in preimplantation development during bovine embryos is currently limited. This study examined the transcript and protein expression patterns of TEAD4 and attempted to elucidate the functions of TEAD4 during bovine preimplantation development using RNA interference. TEAD4 mRNA was found to be upregulated between the 16-cell and morula stages, and nuclear localization of the TEAD4 protein was detected at the morula stage, as well as in subsequent developmental stages. TEAD4 downregulation did not affect embryonic development until the blastocyst stage, and TEAD4-downregulated embryos were capable of forming the TE under both 5% and 21% O2 conditions. Results of gene expression analysis showed that TEAD4 downregulation did not affect the expression levels of POU class 5 transcription factor 1 (OCT-4), NANOG, caudal-type homeobox 2 (CDX2), GATA binding protein 3 (GATA3), and interferon-tau (IFNT). In conclusion, TEAD4 might be dispensable for development until the blastocyst stage and TE differentiation in bovine embryos.

show abstract

“…Up to the 16-cell stage, glucose and pyruvate consumption remain low, but when the compaction phases in the morula and blastocyst stages begin, the consumption of these substrates increases along with oxygen consumption (SOUZA et al, 2015).…”

Section: Embryonic Energy Metabolismmentioning

confidence: 99%

“…In IVP embryos, the presence of bovine serum albumin (BSA) in culture media results in differences in cleavage rates, as shown by BSA replacement of polyvinyl alcohol. Additionally, the amino acid concentration is altered in the presence of BSA, as BSA can cause endocytosis in ET cells, generating lysine, leucine and glutamate, which are directly related to appropriate embryonic development (SOUZA et al, 2015).…”

Section: Embryonic Energy Metabolismmentioning

confidence: 99%

“…This fact can explain the large accumulation of lipid droplets in bovine IVP embryos because of the use of FCS in the culture media in vitro due to the abnormal supply of lipids contained in the serum. Thus, lipid droplets act as modulators of protein availability and lipid signaling in the nucleus (SOUZA et al, 2015).…”

Section: Importance Of Lipids In Embryonic Developmentmentioning

confidence: 99%

See 1 more Smart Citation

Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant?

Dias

Pivato

Dode

2017

SCA

View full text Add to dashboard Cite

For the development of in vitro produced (IVP) as well as in vivo produced bovine embryos, it is extremely important that their energy metabolism works properly because the embryo must be able to metabolize energy substrates that are necessary for producing energy. Lipids play an important role in early embryonic development, acting as source of energy for oocytes and embryos. However, it is known that oocytes and embryos, mainly IVP, accumulate large amounts of lipids in the cytoplasm. Although they are extremely important in embryonic development, lipids have been associated with the reduced survival of bovine embryos following cryopreservation. There is evidence that at least four different categories of lipids affect embryo survival after cryopreservation, including triglycerides (TAG), free fatty acids, cholesterol and phospholipids. Thus, many studies are being conducted to improve the resistance of IVP embryos to the cryopreservation process by reducing the concentration or removing the source of serum from the medium or by reducing oocyte/embryo lipids using mechanical or chemical means. Regarding the use of delipidating agents that reduce the uptake and synthesis of fatty acids (FA) by cells, substances such as phenazine ethosulfate (PES), forskolin, L-carnitine and isomers of conjugated linoleic acid (CLA) have been utilized. This review aims to address important issues related to embryonic energy metabolism, the importance of lipid metabolism and its relation to the cryopreservation of IVP bovine embryos by summarizing the latest research in this field. Key words: Bovine embryos. Cryopreservation. Energy metabolism. Lipids. ResumoPara o desenvolvimento de embriões bovinos produzidos in vitro (PIV) e in vivo, é extremamente importante que o metabolismo energético funcione de maneira adequada, pois o embrião precisa ser capaz de metabolizar os substratos energéticos necessários para a produção de energia. Os lipídios desempenham papel importante no desenvolvimento embrionário inicial, atuando como fonte de energia para oócitos e embriões. Entretanto, sabe-se que oócitos e embriões bovinos, principalmente os PIV, acumulam grande quantidade de lipídios no citoplasma. Apesar de serem extremamente importantes no desenvolvimento embrionário, os lipídios têm sido associados com a reduzida sobrevivência após a criopreservação de embriões bovinos. Existem evidências de que pelo menos quatro classes de lipídios afetam a sobrevivência embrionária pós-criopreservação, sendo os triglicerídeos (TAG), ácidos graxos livres, colesterol e os fosfolipídios. Sendo assim, vários estudos estão sendo realizados com o intuito de melhorar a resistência dos embriões PIV ao processo de criopreservação, realizando a redução ou retirada do soro do meio ou a redução mecânica ou química de lipídios. Com relação ao uso de agentes delipidantes que diminuam a captação e síntese de ácidos graxos (AG) pelas células, substâncias como fenazina etossulfato (PES), forskolin, L-carnitina e isômeros do ácido linoleico conjugado (CLA) t...

show abstract

Aspects of energetic substrate metabolism of in vitro and in vivo bovine embryos

Cited by 18 publications

References 37 publications

Understanding the uterine environment in early pregnancy in cattle: How have the omics en-hanced our knowledge?

Understanding the uterine environment in early pregnancy in cattle: How have the omics en-hanced our knowledge?

Effects of downregulating TEAD4 transcripts by RNA interference on early development of bovine embryos

Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant?

Contact Info

Product

Resources

About