“…Similarly, in the case of the highly ordered, regularly reproducible structure of FLS and SLS the inducing agents, acid glycoprotein and ATP, were not specific. The list includes thrombin, mushroom tyrosinase, clostridium collagenase (inactivated), chondroitin sulfate, sulfated dextran, and gum arabic (16,43). Curiously enough, the first three agents were even more effective and produced more perfect fibrils after they were boiled and treated with 20 per cent trichloracetic acid, the precipitate removed, the acid dialyzed out, solution lyophilized, and the dry powder used.…”
Section: Inducing Agentsmentioning
confidence: 99%
“…In several instances, however, collagen which can be identified by x-ray diffraction and amino acid composition appears to be composed of unstrlated fibrils; the best example being that of the earthworm cuticle (44). In addition, collagen fibrils having axial periods of 220 A have been observed in embryonic tissues and in tissue culture (41,43). Electron microscopic analyses of several different structural forms reconstituted from solution (to be described), led to the characterization of the unit building block or molecule of collagen called tropocollagen (17).…”
Section: The Collagen Fibril and Its Building Block Tropocollagenmentioning
“…Similarly, in the case of the highly ordered, regularly reproducible structure of FLS and SLS the inducing agents, acid glycoprotein and ATP, were not specific. The list includes thrombin, mushroom tyrosinase, clostridium collagenase (inactivated), chondroitin sulfate, sulfated dextran, and gum arabic (16,43). Curiously enough, the first three agents were even more effective and produced more perfect fibrils after they were boiled and treated with 20 per cent trichloracetic acid, the precipitate removed, the acid dialyzed out, solution lyophilized, and the dry powder used.…”
Section: Inducing Agentsmentioning
confidence: 99%
“…In several instances, however, collagen which can be identified by x-ray diffraction and amino acid composition appears to be composed of unstrlated fibrils; the best example being that of the earthworm cuticle (44). In addition, collagen fibrils having axial periods of 220 A have been observed in embryonic tissues and in tissue culture (41,43). Electron microscopic analyses of several different structural forms reconstituted from solution (to be described), led to the characterization of the unit building block or molecule of collagen called tropocollagen (17).…”
Section: The Collagen Fibril and Its Building Block Tropocollagenmentioning
“…S. F. Jackson m eint, daß die ersten F ilam ente passiv durch die Zellbewegungen (W assermann: durch die Innendruckzunahm e des Zytoplasm a) aus der Zelle her ausgedrückt w ürden und in der «groundsubstance», d .i. den Muco-polysacchariden der am orphen Zw ischensubstanz, ihre Reifung er füh ren ; deren Mengen zeigen einen auffallenden Parallelism us m it dem A usm aß der Fibrillendifferenzierung (Randall et al [ 1952], Ver gleich N abelstrang m it Sehnengewebe). Uber den Einfluß der Zellen au f die A usrichtung der Fibrillen innerhalb des Gewebes wird noch zu sprechen sein.…”
Section: B) Die a Uffassuii" Der Neueren Zeitunclassified
“…im H yalin knorpel. Randall et al [1952] beschreiben im erw achsenen G elenk knorpel von H u h n , E lefant, Mensch ohne A ltersangabe und im N asenknorpel des 4][> M onate alten K albes neben Norm alfibrillen und Fasern ein dichtes N etzw erk feinerer (300 500 Ä dicker) F i brillen, welches die S palträum e zwischen ihnen vollständig ausfüllt. An den Fibrillen dieses N etzwerkes konnte eine G liederung nicht nachgewiesen w erden; n u r ganz vereinzelt w ar etw as zu sehen, was als eine P eriodizität von 180-200 A gedeutet w erden konnte.…”
Section: I I I I I 1 I I I I I I I I I I I I I I I I I I T«unclassified
“…Beste von an h aftendem C hondroitinsulfat störten ab er hier die Sieht. D eutlichere B ilder erzielte M artin [1952,1954], einer der M itarbei ter von Randall et al [1952], in einer R eihenuntersuchung am T ibiaknorpel des H ühnerem bryo von 5 1., 19 B ebrütungslagen im Vergleich zur E piphyse des reifen H uhnes. Das N etzw erk m äßig feiner ungegliederter Fibrillen besteht w ährend der ganzen E n t w icklungszeit und auch im reifen K norpel; es wird nach und nach mit gegliederten Fibrillen durchsetzt.…”
Section: I I I I I 1 I I I I I I I I I I I I I I I I I I T«unclassified
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